SHIRLEY VASCONCELOS KOMNINAKIS
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- Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil(2019) CILLI, Audrey; LUCHS, Adriana; LEAL, Elcio; GILL, Danielle; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; LOBATO, Marcia Cristina Alves Brito Sayao; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys daHuman sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols.
- Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil(2019) RIBEIRO, Geovani de Oliveira; MONTEIRO, Fred Julio Costa; REGO, Marlisson Octavio da S.; RIBEIRO, Edcelha Soares D'Athaide; CASTRO, Daniela Funayama de; CASEIRO, Marcos Montani; MARINHO, Robson dos Santos Souza; KOMNINAKIS, Shirley Vasconcelos; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys da; LEAL, ElcioAdvancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
- Detection and Characterization of Enterovirus B73 from a Child in Brazil(2019) RIBEIRO, Geovani de Oliveira; LUCHS, Adriana; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; WITKIN, Steven S.; VILLANOVA, Fabiola; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; COSTA, Antonio Charlys da; LEAL, ElcioEnterovirus B73 is a new member of the Enterovirus B species. First detected in the USA, it has been subsequently identified in China, India, Oman, and the Netherlands. In this study, we characterize the first B73 strain (named TO-127) to be detected in South America. TO-127 was obtained from a child with acute gastroenteritis living in a rural area in Northern Brazil. The subject was not infected with any known enteric pathogens such as norovirus, rotavirus, helminths, or enteric bacteria. Analysis of the nearly full-length TO-127 genome (6993 nt) indicated a 74-75% nucleotide similarity with EV-B73 strains from other countries. Evolutionary analysis suggests that B73 is endemic and widespread.
bookPart HIV e subtipos: etiologia(2021) SABINO, Ester Cerdeira; BARRETO, Claudia Cortese; SANABANI, Sabri Saeed; KOMNINAKIS, Shirley Vasconcelos