SHIRLEY VASCONCELOS KOMNINAKIS

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  • article 7 Citação(ões) na Scopus
    Re-emergence of mayaro virus and coinfection with chikungunya during an outbreak in the state of Tocantins/Brazil
    (2022) MARINHO, Robson dos Santos Souza; DURO, Rodrigo Lopes Sanz; CALDEIRA, Debora Bellini; GALINSKAS, Juliana; MOTA, Manlio Tasso Oliveira; HUNTER, James; TELES, Maria da Aparecida Rodrigues; MILAGRES, Flavio Augusto de Padua; DIAZ, Ricardo Sobhie; KAWAKUBO, Fernando Shinji; KOMNINAKIS, Shirley Vasconcelos
    Objective To perform a molecular screening to detect infections by the mayaro virus and possible coinfections with Chikungunya during an outbreak in the state of Tocantins/Brazil in 2017. Results Of a total 102 samples analyzed in this study, 6 cases were identified with simultaneous infection between mayaro and chikungunya viruses (5.88%). In these 6 samples, the mean Cycle threshold (Ct) for CHIKV was 26.87 (SD +/- 10.54) and for MAYV was 29.58 (SD +/- 6.34). The mayaro sequences generated showed 95-100% identity to other Brazilian sequences of this virus and with other MAYV isolates obtained from human and arthropods in different regions of the world. The remaining samples were detected with CHIKV monoinfection (41 cases), DENV monoinfection (50 cases) and coinfection between CHIKV/DENV (5 cases). We did not detect MAYV monoinfections.
  • article 9 Citação(ões) na Scopus
    Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil
    (2019) RIBEIRO, Geovani de Oliveira; MONTEIRO, Fred Julio Costa; REGO, Marlisson Octavio da S.; RIBEIRO, Edcelha Soares D'Athaide; CASTRO, Daniela Funayama de; CASEIRO, Marcos Montani; MARINHO, Robson dos Santos Souza; KOMNINAKIS, Shirley Vasconcelos; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys da; LEAL, Elcio
    Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.