LEANDRO EZIQUIEL DE SOUZA

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7
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Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 10 de 26
  • conferenceObject
    Impact of sympathectomy upon myocardium
    (2019) PESSOA, F. Fernanda; JORDAO, M. R.; FONSECA, K. C. B.; ZANONI, F.; SALEMI, V. M. C.; RIBEIRO, O. N.; SOUZA, L. E.; FERNANDES, F.; IRIGOYEN, M. C.; MOREIRA, L. F. P.; MADY, C.; RAMIRES, F. J. A.
  • conferenceObject
    IMPACT OF ANGIOTENSIN CONVERTING ENZYME (ACE) GENE LEVELS ON BAROREFLEX IMPAIRMENT AND SYMPATHETIC HYPERACTIVITY AFTER A HIGH FRUCTOSE DIET
    (2017) MORAES-SILVA, Ivana C.; SOUZA, Leandro E. de; CASARINI, Dulce E.; IRIGOYEN, Maria-Claudia
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    Baroreflex and cardiac dysfunctions evaluated by transesophageal echocardiography, baroreflex sensitivity, autonomic control and invasive measurements in rats submitted to sinoaortic denervation
    (2012) SIRVENTE, R. A.; IRIGOYEN, M. C.; SOUZA, L.; MOSTARDA, C.; FUENTE, R. La; CANDIDO, G.; SOUZA, P.; MEDEIROS, A.; MADY, C.; SALEMI, V. M. C.
    Purpose: Sympathetic hyperactivity commonly seems to be related to cardiac dysfunction and baro and chemoreflexes impairment in hypertension. However, myocardial function has not been evaluated regarding the association of hypertension and baroreflex dysfunction using transesophageal echocardiography. Methods: Exercise test (ET), baroreflex sensitivity, cardiovascular autonomic control, transthoracic and transesophageal echocardiography using intracardiac echocardiographic catheter (AcuNav, Siemens, Mountain View, CA, USA), and invasively biventricular end-diastolic pressures (EDP) were evaluated in rats 10 weeks after sinoaortic denervation (SAD). The rats (n=32) were divided in 4 groups: 16 Wistar (W) with (n=8) or without SAD (n=8) and 16 spontaneously hypertensive rats (SHR) with (n=8) or without SAD (n=8). Results: Blood pressure (BP) and heart rate (HR) did not show any change between the groups SAD and without SAD, although, SHR showed higher BP levels in comparison to W. BP variability was increased in SHR groups compared to W. After SAD, BP variability increased in all groups compared to W (W: 15 mmHg2; *DSA: 49 mmHg2; *SHR: 60 mmHg2; *SHR-SAD:137 mmHg2, *p<0.05 vs. W). Exercise tests results showed that SHR had better functional capacity compared to SAD and SHRSAD (W: 1.16m/s; DSA: 0.9m/s; *SHR: 1.46; SHR-DSA: 1.02, *p<0.05 vs. SAD and SHRSAD). Left ventricular concentric hypertrophy, segmental systolic dysfunction and global diastolic LV dysfunction, segmental and global systolic dysfunction, and global diastolic RV dysfunction, indirect signals of pulmonary arterial hypertension were shown by echocardiography, mostly evident in SHRSAD. The RV-EDP increased in all groups compared to W(W:3±0.39mmHg, *SAD:4.7±0.52mmHg, *SHR: 6.6±1.1mmHg, *SHRSAD:7.8±0.87mmHg, *p<0.05 vs. W), and LV-EDP increased in SHR and SHRSAD groups compared to W, and in SHRSAD compared to SAD (W: 5,83±0,19 mmHg,SAD: 8.98±1.2 mmHg, *SHR: 12.51±4.73 mmHg, *#SHRSAD: 14.57±2.52mmHg, *p<0.05vs.W,#p<0.05 vs. DSA). There was a relation between invasive or noninvasive measurements of RV showing good accuracy of echocardiographic measurements. Conclusions: Our results suggest that baroreflex dysfunction impaired biventricular function. Moreover, the findings of RV dysfunction indicate that SAD may lead to increased pulmonary artery pressure, supporting a role for baroreflex dysfunction in the pathogenesis of the hypertensive cardiac disease.
  • conferenceObject
    Effects of aerobic exercise training on obese female mice: metabolic and autonomic evaluation
    (2013) SARTORI, Michelle; SOUZA, Leandro Eziquiel de; SOUZA, Pamella Ramona de; SANTOS, Fernando; ANGELIS, Katia De; IRIGOYEN, Maria Claudia
    The aim of this study was to evaluate the metabolic and autonomic parameters in trained youth obese mice. Female ob/ob mice (4 weeks old) were randomized into sedentary (OS, n=11) or trained (OT, n=8) (treadmill,5 days/w, 60 min/d, during 8 wks) groups. Blood glucose was measured by reagent strips. Blood pressure signals were recorded using a data acquisition system. The OT group had higher exercise capacity compared to the OS group. OT group had reduced body weight, white adipose tissue and blood glucose (45±2g, 6.1±1g and 133±3g mg/dL) compared to OS group (50±2g, 6.1±1g and 169±8 mg/dL). Exercise training improved glucose tolerance in trained group (OS: 27961±6501 vs. OT: 18258±6283 mg/dL/min). Mean arterial pressure and heart rate were similar between groups. The trained group showed increase in heart rate variability compared with sedentary group (OT: 50±12 vs. OS: 33±8ms2). Blood pressure variability and low frequency band were higher in OS group (23 ± 3mmHg2 and 7 ± 1 mmHg2) compared to OT (15 ± 2 mmHg2 and 5 ± 1 mmHg2). Moreover, the spontaneous baroreflex sensitivity, represented by alpha index, was lower in sedentary group (1.2 ± 0.3 ms/mmHg) compared to trained group (1.9 ± 0.2 ms/mmHg). In conclusion, our results suggest that physical training during lifespan can be effective inattenuating the increase in weight and blood glucose and improving blood pressure variability in obese mice.
  • article 23 Citação(ões) na Scopus
    Cardiac dysfunction in Pkd1-deficient mice with phenotype rescue by galectin-3 knockout
    (2016) BALBO, Bruno E.; AMARAL, Andressa G.; FONSECA, Jonathan M.; CASTRO, Isac de; SALEMI, Vera M.; SOUZA, Leandro E.; SANTOS, Fernando dos; IRIGOYEN, Maria C.; QIAN, Feng; CHAMMAS, Roger; ONUCHIC, Luiz F.
    Alterations in myocardial wall texture stand out among ADPKD cardiovascular manifestations in hypertensive and normotensive patients. To elucidate their pathogenesis, we analyzed the cardiac phenotype in Pkd1(cond/cond) Nestin(cre) (CYG+) cystic mice exposed to increased blood pressure, at 5 to 6 and 20 to 24 weeks of age, and Pkd1(+/-) (HTG+) noncystic mice at 5-6 and 10-13 weeks. Echocardiographic analyses revealed decreased myocardial deformation and systolic function in CYG+ and HTG+ mice, as well as diastolic dysfunction in older CYG+ mice, compared to their Pkd1(cond/cond) and Pkd1(+/+) controls. Hearts from CYG+ and HTG+ mice presented reduced polycystin-1 expression, increased apoptosis, and mild fibrosis. Since galectin-3 has been associated with heart dysfunction, we studied it as a potential modifier of the ADPKD cardiac phenotype. Double-mutant Pkd1(cond/cond):Nestin(cre);Lgals(3-/-) (CYG-) and Pkd1(+/-);Lgals(3-/-) (HTG-) mice displayed improved cardiac deformability and systolic parameters compared to single -mutants, not differing from the controls. CYG- and HTG- showed decreased apoptosis and fibrosis. Analysis of a severe cystic model (Pkd1(v/v); VVG+) showed that Pkd1(v/v);Lgals(3-/-) (VVG-) mice have longer survival, decreased cardiac apoptosis and improved heart function compared to VVG+. CYG- and VVG- animals showed no difference in renal cystic burden compared to CYG+ and VVG+ mice. Thus, myocardial dysfunction occurs in different Pkdl-deficient models and suppression of galectin-3 expression rescues this phenotype.
  • article 7 Citação(ões) na Scopus
    Smoking accelerates renal cystic disease and worsens cardiac phenotype in Pkd1-deficient mice
    (2021) SOUSA, Marciana V.; AMARAL, Andressa G.; FREITAS, Jessica A.; MURATA, Gilson M.; WATANABE, Elieser H.; BALBO, Bruno E.; TAVARES, Marcelo D.; HORTEGAL, Renato A.; ROCON, Camila; SOUZA, Leandro E.; IRIGOYEN, Maria C.; SALEMI, Vera M.; ONUCHIC, Luiz F.
    Smoking has been associated with renal disease progression in ADPKD but the underlying deleterious mechanisms and whether it specifically worsens the cardiac phenotype remain unknown. To investigate these matters, Pkd1-deficient cystic mice and noncystic littermates were exposed to smoking from conception to 18 weeks of age and, along with nonexposed controls, were analyzed at 13-18 weeks. Renal cystic index and cyst-lining cell proliferation were higher in cystic mice exposed to smoking than nonexposed cystic animals. Smoking increased serum urea nitrogen in cystic and noncystic mice and independently enhanced tubular cell proliferation and apoptosis. Smoking also increased renal fibrosis, however this effect was much higher in cystic than in noncystic animals. Pkd1 deficiency and smoking showed independent and additive effects on reducing renal levels of glutathione. Systolic function and several cardiac structural parameters were also negatively affected by smoking and the Pkd1-deficient status, following independent and additive patterns. Smoking did not increase, however, cardiac apoptosis or fibrosis in cystic and noncystic mice. Notably, smoking promoted a much higher reduction in body weight in Pkd1-deficient than in noncystic animals. Our findings show that smoking aggravated the renal and cardiac phenotypes of Pkd1-deficient cystic mice, suggesting that similar effects may occur in human ADPKD.
  • article 7 Citação(ões) na Scopus
    Cardiovascular autonomic dysfunction in non-obese diabetic mice
    (2013) MORAES, Oscar A.; COLUCCI, Juliana A.; SOUZA, Leandro E.; SCAPINI, Katia B.; MORAES-SILVA, Ivana C.; MOSTARDA, Cristiano; ANGELIS, Katia De; CASARINI, Dulce E.; IRIGOYEN, Maria Claudia
    It is known that diabetes is associated with autonomic dysfunction; however, data about autonomic function in non-obese diabetic mice (NOD) remain scarce. We evaluated the autonomic profile of NOD mice. Female mice, 24-28 week old, were divided in two groups: NOD (n = 6) and control (n = 6, Swiss mice). NOD mice with glycemia >= 300 mg/dl were used. Heart rate variability (HRV) and arterial pressure variability (APV) in time and frequency domains, symbolic analysis of heart rate (HR) and baroreflex sensitivity were evaluated. HR and arterial pressure (AP) were similar between the groups; however, HRV (total variance of RR interval: NOD = 21.07 +/- 3.75 vs. C = 42.02 +/- 6.54 ms(2)) and the vagal modulation index RMSSD were lower in NOD group (4.01 +/- 032 vs. 8.28 +/- 0.97 ms). Moreover, the absolute and normalized low-frequency (LF) components were also enhanced in NOD (normalized = 61.0 +/- 4.0%) as compared to control mice (normalized = 20.0 +/- 4.0%). Both the absolute and normalized high-frequency (HF) components were lower in NOD (normalized = 39.0 +/- 4.0%) when compared to the control group (normalized = 80.0 +/- 4.0). In the symbolic analysis the 0V pattern, an indication of sympathetic activity, was higher in NOD and 2LV pattern, an indication of parasympathetic activity, was lower in the NOD than in the control group. Both bradycardic and tachycardic responses were decreased in NOD (3.01 +/- 0.72 vs. 4.54 +/- 0.36 bpm/mm Hg and 2.49 +/- 031 vs. C = 3.43 +/- 033 bpm/mm Hg) when compared to the control group. Correlation analysis showed negative correlations between vagal indexes (RMSSD, %HF and 2LV) and glycemic levels. In conclusion, NOD mice develop severe diabetes correlated with autonomic dysfunction.
  • conferenceObject
    PROINFLAMMATORY ROLE OF ANGIOTENSIN II IN THE AORTA OF NORMOTENSIVE MICE
    (2018) LIMA, R. S.; SILVA, J. C. S.; LIMA, C. T.; SOUZA, L. E.; BALADI, M. G.; IRIGOYEN, M. C.; LACCHINI, S.
  • article 0 Citação(ões) na Scopus
    Interval and continuous aerobic exercise training similarly increase cardiac function and autonomic modulation in infarcted mice (vol 13, pg 257, 2017)
    (2017) ABAD, Cesar Cavinato Cal; NASCIMENTO, Ademir Manuel do; SOUZA, Leandro Eziquiel de; FIGUEROA, Diego; RAMONA, Pamella; SARTORI, Michele; SCAPINI, Katia B.; ALBUQUERQUE, Oscar; MORAES-SILVA, Ivana Cinthya; COELHO-JUNIOR, Helio Jose; RODRIGUES, Bruno; MOSTARDA, Cristiano Teixeira; ANGELIS, Katia De; IRIGOYEN, Maria Claudia
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    The metabolic mechanisms involved in the heart protection from myocardial infarction induced by diabetes
    (2012) MALFITANO, Christiane; CARBONARO, M.; SOUZA JUNIOR, A. L. de; ALBA-LOUREIRO, T. C.; SOUZA, L. E.; FIGUEROA, D.; SILVA, K.; CURI, R.; IRIGOYEN, M. C.