GILKA JORGE FIGARO GATTAS

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Departamento de MedicinaLegal, Ética Médica e Medicina Social e do Trabalho, Faculdade de Medicina - Docente
LIM/40 - Laboratório de Imunohematologia e Hematologia Forense, Hospital das Clínicas, Faculdade de Medicina - Líder

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Agora exibindo 1 - 10 de 17
  • article 12 Citação(ões) na Scopus
    Arginine intake is associated with oxidative stress in a general population
    (2017) CARVALHO, Aline Martins de; OLIVEIRA, Antonio Anax Falcao de; LOUREIRO, Ana Paula de Melo; GATTAS, Gilka Jorge Figaro; FISBERG, Regina Mara; MARCHIONI, Dirce Maria
    Objective: The aim of this study was to assess the association between protein and arginine from meat intake and oxidative stress in a general population. Methods: Data came from the Health Survey for Sao Paulo (ISA-Capital), a cross-sectional population based study in Brazil (N = 549 adults). Food intake was estimated by a 24-h dietary recall. Oxidative stress was estimated by malondialdehyde (MDA) concentration in plasma. Analyses were performed using general linear regression models adjusted for some genetic, lifestyle, and biochemical confounders. Results: MDA levels were associated with meat intake (P for linear trend = 0.031), protein from meat (P for linear trend = 0.006), and arginine from meat (P for linear trend = 0.044) after adjustments for confounders: age, sex, body mass index, smoking, physical activity, intake of fruit and vegetables, energy and heterocyclic amines, C-reactive protein levels, and polymorphisms in GSTM1 (glutathione S-transferase Mu 1) and GSTT1 (glutathione S-transferase theta 1) genes. Results were not significant for total protein and protein from vegetable intake (P > 0.05). Conclusions: High protein and arginine from meat intake were associated with oxidative stress independently of genetic, lifestyle, and biochemical confounders in a population-based study. Our results suggested a novel link between high protein/arginine intake and oxidative stress, which is a major cause of age -related diseases.
  • article 32 Citação(ões) na Scopus
    European ancestry and polymorphisms in DNA repair genes modify the risk of melanoma: A case-control study in a high UV index region in Brazil
    (2011) GONCALVES, Fernanda T.; FRANCISCO, Guilherme; SOUZA, Sonia P. de; LUIZ, Olinda C.; FESTA-NETO, Cyro; SANCHES, Jose A.; CHAMMAS, Roger; GATTAS, Gilka J. F.; ELUF-NETO, Jose
    Background: UV radiation is the major environmental factor related to development of cutaneous melanoma. Besides sun exposure and the influence of latitude, some host characteristics such as skin phototype and hair and eye color are also risk factors for melanoma. Polymorphisms in DNA repair genes could be good candidates for susceptibility genes, mainly in geographical regions exposed to high solar radiation. Objective: Evaluate the role of host characteristic.; and DNA repair polymorphism in melanoma risk in Brazil. Methods: We carried out a hospital-based case-control study in Brazil to evaluate the contribution of host factors and polymorphisms in DNA repair to melanoma risk. A total of 412 patients (202 with melanoma and 210 controls) were analyzed regarding host characteristics for melanoma risk as well as for 11 polymorphisms in DNA repair genes. Results: We found an association of host characteristics with melanoma development, such as eye and hair color, fair skin, history of pigmented lesions removed, sunburns in childhood and adolescence, and also European ancestry. Regarding DNA repair gene polymorphisms, we found protection for the XPG 1104 His/His genotype (OR 0.32; 95% CI 0.13-0.75), and increased risk for three polymorphisms in the XPC gene (PAT+; IV-6A and 939Gln), which represent a haplotype for XPC. Melanoma risk was higher in individuals carrying the complete XPC haplotype than each individual polymorphism (OR 3.64; 95% CI 1.77-7.48). Conclusions: Our data indicate that the host factors European ancestry and XPC polymorphisms contributed to melanoma risk in a region exposed to high sun radiation.
  • article 12 Citação(ões) na Scopus
    Ethnicity and Cutaneous Melanoma in the City of Sao Paulo, Brazil: A Case-Control Study
    (2012) LUIZ, Olinda C.; GIANINI, Reinaldo Jose; GONCALVES, Fernanda T.; FRANCISCO, Guilherme; FESTA-NETO, Cyro; SANCHES, Jose Antonio; GATTAS, Gilka J. F.; CHAMMAS, Roger; ELUF-NETO, Jose
    Background: Over the last century the incidence of cutaneous melanoma has increased worldwide, a trend that has also been observed in Brazil. The identified risk factors for melanoma include the pattern of sun exposure, family history, and certain phenotypic features. In addition, the incidence of melanoma might be influenced by ethnicity. Like many countries, Brazil has high immigration rates and consequently a heterogenous population. However, Brazil is unique among such countries in that the ethnic heterogeneity of its population is primarily attributable to admixture. This study aimed to evaluate the contribution of European ethnicity to the risk of cutaneous melanoma in Brazil. Methodology/Principal Findings: We carried out a hospital-based case-control study in the metropolitan area of Sao Paulo, Brazil. We evaluated 424 hospitalized patients (202 melanoma patients and 222 control patients) regarding phenotypic features, sun exposure, and number of grandparents born in Europe. Through multivariate logistic regression analysis, we found the following variables to be independently associated with melanoma: grandparents born in Europe-Spain (OR = 3.01, 95% CI: 1.03-8.77), Italy (OR = 3.47, 95% CI: 1.41-8.57), a Germanic/Slavic country (OR = 3.06, 95% CI: 1.05-8.93), or >= 2 European countries (OR = 2.82, 95% CI: 1.06-7.47); eye color-light brown (OR = 1.99, 95% CI: 1.14-3.84) and green/blue (OR = 4.62; 95% CI 2.22-9.58); pigmented lesion removal (OR = 3.78; 95% CI: 2.21-6.49); no lifetime sunscreen use (OR = 3.08; 95% CI: 1.03-9.22); and lifetime severe sunburn (OR = 1.81; 95% CI: 1.03-3.19). Conclusions: Our results indicate that European ancestry is a risk factor for cutaneous melanoma. Such risk appears to be related not only to skin type, eye color, and tanning capacity but also to others specific characteristics of European populations introduced in the New World by European immigrants.
  • conferenceObject
    Effect of heterocyclic aminesfrom meat intake on oxidative stress according to GSTT1 polymorphism
    (2015) CARVALHO, Aline; CARIOCA, Antonio Augusto; STELUTI, Josiane; LOUREIRO, Ana Paula; GATTAS, Gilka; FISBERG, Regina; QI, Lu; MARCHIONI, Dirce
  • article 4 Citação(ões) na Scopus
    Exploring the Role of Alcohol Metabolizing Genotypes in a 12-Week Clinical Trial of Naltrexone for Alcohol Use Disorder
    (2021) CASTALDELLI-MAIA, Joao M.; MALBERGIER, Andre; OLIVEIRA, Adriana B. P. de; AMARAL, Ricardo A.; NEGRAO, Andre B.; GONCALVES, Priscila D.; VENTRIGLIO, Antonio; BERARDIS, Domenico de; ANTONIO, Juliana de; FIRIGATO, Isabela; GATTAS, Gilka J. F.; GONCALVES, Fernanda de Toledo
    Background: The efficacy of naltrexone in the treatment of alcohol use disorder (AUD) has been associated with a set of variables not directly related with the expression of opioid receptors. All the variables have been found to be highly associated with AUD itself or more severe clinical levels of AUD. Objectives: Given the high association between alcohol metabolizing enzymes (AME) and the outcome of AUD, the present study aims to investigate the role of AME genotype variants in the treatment of AUD with naltrexone. Methods: We carried out a 12-week longitudinal clinical trial based on the treatment of AUD patients with naltrexone (N = 101), stratified by different alcohol metabolization genotypes. Genotyping was performed after the inclusion of the patients in the study, based on the individual presence of single nucleotide polymorphisms (SNPs) in the ADH (alcohol dehydrogenase)1B (ADH1B*2 and ADH1B*3), ADH1C (ADHC*1) and ALDH (aldehyde dehydrogenase) 2 (ALDH2*2) genes. The outcome of alcohol use has been monitored employing the timeline follow-back during the treatment. Results: The ADH1C*1 (Ile350Val, rs698) and ALDH2*2 (Glu504Lys, rs671) polymorphisms were associated with a better response to naltrexone treatment, whereas the ADH1B*3 (Arg370Cys, rs2066702) allelic variant showed a negative outcome. Conclusions: The present study explores a genomic setting for the treatment of AUD with naltrexone. According to our findings, the association between ADH1C*1 and ALDH2*2 variants and better outcomes suggests a successful treatment, whereas the ADH1B*3 mutated allele might lead to an unsuccessful treatment. Further studies should be performed to investigate the relationship between alcohol metabolizing genotypes, the family history of alcohol use disorders and the effect of naltrexone on the outcomes. Genotyping may be a valuable tool for precision-medicine and individualized approach, especially in the context of alcohol use disorders. The small number of subjects was the main limitation of the present study.
  • article 290 Citação(ões) na Scopus
    The HUman MicroNucleus project on eXfoLiated buccal cells (HUMNXL): The role of life-style, host factors, occupational exposures, health status, and assay protocol
    (2011) BONASSI, Stefano; COSKUN, Erdem; CEPPI, Marcello; LANDO, Cecilia; BOLOGNESI, Claudia; BURGAZ, Sema; HOLLAND, Nina; KIRSH-VOLDERS, Micheline; KNASMUELLER, Siegfried; ZEIGER, Errol; CARNESOLTAS, Deyanira; CAVALLO, Delia; SILVA, Juliana da; ANDRADE, Vanessa M. de; DEMIRCIGIL, Gonca Cakmak; ODIO, Anibal Dominguez; DONMEZ-ALTUNTAS, Hamiyet; GATTAS, Gilka; GIRI, Ashok; GIRI, Sarbani; GOMEZ-MEDA, Belinda; GOMEZ-ARROYO, Sandra; HADJIDEKOVA, Valeria; HAVERIC, Anja; KAMBOJ, Mala; KURTESHI, Kemajl; MARTINO-ROTH, Maria Grazia; MONTOYA, Regina Montero; NERSESYAN, Armen; PASTOR-BENITO, Susana; SALVADORI, Daisy Maria Favero; SHAPOSHNIKOVA, Alina; STOPPER, Helga; THOMAS, Philip; TORRES-BUGARIN, Olivia; YADAV, Abhay Singh; GONZALEZ, Guillermo Zuniga; FENECH, Michael
    The human buccal micronucleus cytome assay (BMCyt) is one of the most widely used techniques to measure genetic damage in human population studies. Reducing protocol variability, assessing the role of confounders, and estimating a range of reference values are research priorities that will be addressed by the HUMNXL, collaborative study. The HUMNXL, project evaluates the impact of host factors, occupation, life-style, disease status, and protocol features on the occurrence of MN in exfoliated buccal cells. In addition, the study will provide a range of reference values for all cytome endpoints. A database of 5424 subjects with buccal MN values obtained from 30 laboratories worldwide was compiled and analyzed to investigate the influence of several conditions affecting MN frequency. Random effects models were mostly used to investigate MN predictors. The estimated spontaneous MN frequency was 0.74 parts per thousand (95% CI 0.52-1.05). Only staining among technical features influenced MN frequency, with an abnormal increase for non-DNA-specific stains. No effect of gender was evident, while the trend for age was highly significant (p < 0.001). Most occupational exposures and a diagnosis of cancer significantly increased MN and other endpoints frequencies. MN frequency increased in heavy smoking (>= 40 cig/day. FR = 1.37:95% CI 1.03-.82) and decreased with daily fruit consumption (FR = 0.68; 95% CI 0.50-0.91). The results of the HUMNXL, project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.
  • article 3 Citação(ões) na Scopus
    Many hands make light work: CNV of GSTM1 effect on the oral carcinoma risk
    (2022) FIRIGATO, Isabela; LOPEZ, Rossana V. M.; CURIONI, Otavio A.; ANTONIO, Juliana De; GATTAS, Gilka Figaro; GONCALVES, Fernanda de Toledo
    Background: Genetic alterations of oral squamous cell carcinoma (OSCC) allow the understanding of the oral carcinogenesis and the identification of molecular biomarkers that aid the early diagnosis of the disease. The copy number variation (CNV) of GSTM1 and GSTT1 are promising targets because these two genes codify enzymes that perform the inactivation of tobacco carcinogens, which are the main risk factor of OSCC. However, the different levels of - detoxification mechanism in relation to each copy of the genes are unknown. Therefore, this study aimed to investigate the possible association of the CNV of GSTM1 and GSTT1 with the risk of development of OSCC. Methods: A total of 234 OSCC patients and 422 patients without any cancer diagnoses were recruited from Heli acute accent opolis Hospital from 2000 to 2011. The CNV was determined by TaqMan real-time PCR and the CopyCaller software. Odds ratio (OR) and 95% confidence interval (95% CI) values were calculated by Multiple Logistic Regression. Results: Most OSCC patients reported they continued smoking high amounts of cigarettes despite the tumor diagnosis. The CNV of GSTM1 varied from zero to two copies and the analysis revealed that two copies of GSTM1 decreased by 53% the OSCC risk (OR 0.47; 95% CI 0.24-0.92) and the risk of the tumor was modified according to the interaction of the CNV of GSTM1 and the cigarette smoking consumption, which for the amount of 40 packs-year of cigarettes the OSCC risk diminished progressively according to the increase of copies of GSTM1. Although the GSTT1 gene varied from zero to three copies, none of them were associated with the tumor risk. Conclusion: The findings suggest that the CNV of GSTM1 might be applied as a tool for the surveillance of patients and the early detection of OSCC.
  • article 2 Citação(ões) na Scopus
    Interação entre consumo alimentar e polimorfismos da GSTM1 e GSTT1 no risco para o câncer de cabeça e pescoço: estudo caso-controle em São Paulo, Brasi
    (2011) MARCHIONI, Dirce Maria Lobo; GATTAS, Gilka Jorge Figaro; CURIONI, Otavio A.; CARVALHO, Marcos Brasilino de
    A hospital-based case-control study was conducted to investigate the potential interaction between dietary factors and polymorphisms in phase II metabolic enzymes GSTM1 and GSTT1, associated with head and neck cancer risk. The study included 103 histologically confirmed incident cases and 101 controls. Food intake was estimated with a validated food frequency questionnaire. The gene polymorphisms were evaluated by PCR. Increased risk was observed in the highest tertile of beef consumption in the presence of the GSTM1 (OR = 10.79; 95% CI: 2.17-53.64) and GSTT1 null alleles (OR = 3.41; 95% CI: 0.43-27.21). Assessment of dietary intake considering the ratio between animal product and vegetable consumption showed OR = 2.35 (95% CI: 0.27-19.85) in the intermediate tertile and OR = 3.36 (95% CI: 0.412-7.03) in the highest tertile. The results suggest a possible interaction between meat intake and GSTM1/GSTT1 polymorphisms in modulating the risk of head and neck cancer, influenced by vegetable consumption.
  • article 18 Citação(ões) na Scopus
    Genetic Variants Involved in One-Carbon Metabolism: Polymorphism Frequencies and Differences in Homocysteine Concentrations in the Folic Acid Fortification Era
    (2017) STELUTI, Josiane; CARVALHO, Aline M.; CARIOCA, Antonio A. F.; MIRANDA, Andreia; GATTAS, Gilka J. F.; FISBERG, Regina M.; MARCHIONI, Dirce M.
    Folate and other B vitamins are essential co-factors of one-carbon metabolism, and genetic variants, such as polymorphisms, can alter the metabolism. Furthermore, the adoption of food fortification with folic acid showed a decrease of homocysteine concentration. The aim of this study was to investigate the frequencies of the polymorphisms of enzymes and carrier proteins involved in one-carbon metabolism, and to evaluate homocysteine concentrations in the presence of these genetic variants in a population exposed to mandatory food fortification with folic acid. Using data from a population-based cross-sectional study in Sao Paulo, Brazil, the study population comprised 750 participants above 12 years of age of both genders. A linear regression model was used to evaluate the homocysteine concentrations according to genetic variants and folate level. The results showed that the minor allelic frequencies were 0.33 for MTHFR (rs1801133), 0.24 for MTHFR (rs1801131), 0.19 for MTR (rs1805087), 0.42 for MTRR (rs1801394), 0.46 for RFC1 (rs1051266), and 0.47 for DHFR (19-bp deletion). The genetic variants of MTHFR 677C> T, MTRR 66A> G and RFC-1 80G> A were different according to race. The homocysteine concentrations increased in the CT and TT compared to CC genotypes of polymorphism MTHFR 677C> T in all populations, and differences between the homocysteine concentrations according to the genotypes of MTHFR 677C> T were observed regardless of folate level.
  • article 0 Citação(ões) na Scopus
    Age-related craniofacial differences based on spatio-temporal face image atlases
    (2019) XAVIER, Igor R. R.; GIRALDI, Gilson A.; GIBSON, Stuart James; GATTAS, Gilka J. F.; RUECKERT, Daniel; THOMAZ, Carlos E.
    A number of studies have been developed recently in order to explore associations between craniofacial differences and genetics. Most of these works have been based on spatial face image models, adjusted for the counter effects of age. This approach provides a limited understanding of normal and abnormal craniofacial development owing to the lack of age progression information. Here, the authors propose and implement an imaging framework that combines facial landmark positioning, non-rigid registration, novel age-dependent face modelling and common distance metrics to disclose the most facial differences that vary across the time due to the subjects' age. All the experiments carried out and corresponding results presented here are based on a database comprising ordinary two-dimensional (2D) frontal face images of Down Syndrome (DS) and control sample groups. A number of craniofacial metrics have been successfully identified that highlight statistically significant and clinically relevant differences between the controls and the faces associated with DS within the age range from 1 to 18 years old, producing realistic unbiased face models with similar level of detail at all age-intervals, despite the small sample size available.