CAMILA RODRIGUES MORENO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
conferenceObject SERUM ACTIVE COLLAGENASE FROM PATHOGENIC ARCHAEAL COLLAGENASE MAY CAUSE HEART FAILURE IN CHAGASIC PATIENTS(2019) HIGUCHI, Maria De Lourdes; KAWAKAMI, Joyce; IKEGAMI, Renata; REIS, Marcia; MORENO, Camila R.; PEREIRA, Jaqueline; IANNI, Barbara; BUCK, Paula; SANTOS, Marilia; BOCCHI, Edimar- Distinct Microbial Communities in Dilated Cardiomyopathy Explanted Hearts Are Associated With Different Myocardial Rejection Outcomes(2021) PEREIRA, Jaqueline de Jesus; IKEGAMI, Renata Nishiyama; KAWAKAMI, Joyce Tiyeko; GARAVELO, Sherrira Menezes; REIS, Marcia Martins; PALOMINO, Suely Aparecida Pinheiro; MANGINI, Sandrigo; MORENO, Camila Rodrigues; BARROS, Samar Freschi de; SOUZA, Aline Rodrigues; HIGUCHI, Maria de LourdesBackgroundIdiopathic dilated cardiomyopathy (IDCM) myocardial inflammation may be associated with external triggering factors such as infectious agents. Here, we searched if moderate/severe heart transplantation rejection is related to the presence of myocardial inflammation in IDCM explanted hearts, associated with microbial communities. MethodReceptor myocardial samples from 18 explanted hearts were separated into groups according to post-transplant outcome: persistent moderate rejection (PMR; n = 6), moderate rejection (MR; n = 7) that regressed after pulse therapy, and no rejection (NR; n = 5)/light intensity rejection. Inflammation was quantified through immunohistochemistry (IHC), and infectious agents were evaluated by IHC, molecular biology, in situ hybridization technique, and transmission electron microscopy (TEM). ResultsNR presented lower numbers of macrophages, as well as B cells (p = 0.0001), and higher HLA class II expression (p <= 0.0001). PMR and MR showed higher levels of Mycoplasma pneumoniae (p = 0.003) and hepatitis B core (p = 0.0009) antigens. NR presented higher levels of parvovirus B19 (PVB19) and human herpes virus 6 (HHV6) and a positive correlation between Borrelia burgdorferi (Bb) and enterovirus genes. Molecular biology demonstrated the presence of M. pneumoniae, Bb, HHV6, and PVB19 genes in all studied groups. TEM revealed structures compatible with the cited microorganisms. ConclusionsThis initial study investigating on infectious agents and inflammation in the IDCM explanted hearts showed that the association between M. pneumoniae and hepatitis B core was associated with a worse outcome after HT, represented by MR and PMR, suggesting that different IDCM microbial communities may be contributing to post-transplant myocardial rejection.
conferenceObject Early Biomarkers to Predict a Worse Outcome After Acute Myocardial Infarction: Circulating Infectious Extracellular Vesicles From Archaea and M. Pneumoniae Are Present and Correlated to Cytokines IL-6 and IL-10(2022) MORENO, Camila R.; SOEIRO, Alexandre M.; SATO, Maria N.; PEREIRA, Jaqueline J.; IKEGAMI, Renata N.; KAWAKAMI, Joyce T.; REIS, Marcia M.; OLIVEIRA, Luanda M.; HIGUCHI, Maria Lourdes- Morphomolecular Characterization of Serum Nanovesicles From Microbiomes Differentiates Stable and Infarcted Atherosclerotic Patients(2021) MORENO, Camila Rodrigues; RAMIRES, Jose Antonio Franchini; LOTUFO, Paulo Andrade; SOEIRO, Alexandre Matos; OLIVEIRA, Luanda Mara da Silva; IKEGAMI, Renata Nishiyama; KAWAKAMI, Joyce Tiyeko; PEREIRA, Jaqueline de Jesus; REIS, Marcia Martins; HIGUCHI, Maria de LourdesMicrobial communities are considered decisive for maintaining a healthy situation or for determining diseases. Acute myocardial infarction (AMI) is an important complication of atherosclerosis caused by the rupture of atheroma plaques containing proinflammatory cytokines, reactive oxygen species, oxidized low-density lipoproteins (oxLDL), damaged proteins, lipids, and DNA, a microenvironment compatible with a pathogenic microbial community. Previously, we found that archaeal DNA-positive infectious microvesicles (iMVs) were detected in vulnerable plaques and in the sera of Chagas disease patients with heart failure. Now, we characterize and quantify the levels of serum microbiome extracellular vesicles through their size and content using morphomolecular techniques to differentiate clinical outcomes in coronary artery disease (CAD). We detected increased numbers of large iMVs (0.8-1.34 nm) with highly negative surface charge that were positive for archaeal DNA, Mycoplasma pneumoniae antigens and MMP9 in the sera of severe AMI patients, strongly favoring our hypothesis that pathogenic archaea may play a role in the worst outcomes of atherosclerosis. The highest numbers of EVs <100 nm (exosomes) and MVs from 100 to 200 nm in the stable atherosclerotic and control healthy groups compared with the AMI groups were indicative that these EVs are protective, entrapping and degrading infectious antigens and active MMP9 and protect against the development of plaque rupture. Conclusion: A microbiome with pathogenic archaea is associated with high numbers of serum iMVs in AMI with the worst prognosis. This pioneering work demonstrates that the morphomolecular characterization and quantification of iEVs in serum may constitute a promising serum prognostic biomarker in CAD.
conferenceObject Active MMP9 Associated to Low MMP9 Removal by Exosomes in the Serum Characterized Patients With Fatal Acute Myocardial Infarction(2018) HIGUCHI, Maria Lourdes; MORENO, Camila R.; IKEGAMI, Renata N.; YOSHIKAWA, Fabio S.; PEREIRA, Jaqueline J.; PALOMINO, Suely A.; BENSENOR, Isabela J.; LOTUFO, Paulo A.; SATO, Maria N.- Study of laser fluorescence spectroscopy in livers of rats with hypothermic ischemia(2023) TSUGE, Allison Takeo; PEREIRA, Jaqueline de Jesus; VOLLET-FILHO, Jose Dirceu; KUBRUSLY, Marcia Saldanha; GALVAO, Flavio Henrique Ferreira; RIBEIRO, Orlando Nascimento; MORENO, Camila Rodrigues; IKEGAMI, Renata Nishiyama; CHAIB, Eleazar; SILVA, Orlando de Castro ePurpose: After partial hepatectomy (PH), the remaining liver (RL) undergoes regenerative response proportional to the host. Limited literature exists on hepatic viability after tissue injury during hypothermic preservation. Spectroscopy measures cellular fluorescence and is explored for tissue characterization and parameter investigation. This study aimed to assess fluorescence analysis (spectroscopy) in evaluating liver viability and its relationship with hepatic tissue regeneration 24 hours after PH. Additionally, we analyzed liver regeneration in RL after 70% partial hepatectomy under hypothermic conditions with laser irradiation. Methods: Fifty-six Wistar rats were divided into four groups: total non-perfused liver (control), total perfused liver, partial hepatectomy ""in situ"", and partial hepatectomy ""ex situ"". Tissue analysis was performed at 0 and 24 hours using spectroscopy with laser devices emitting at 532 (green) and 405 nm (violet). Results: Spectroscopy identified tissue viability based on consistent results with Ki67 staining. The fluorescence spectra and Ki67 analysis displayed similar patterns, linking proliferative activity and absorption intensity. Conclusion: Fluorescence spectroscopy proves to be promising for real-time analysis of cellular activity and viability. Metabolic activity was observed in groups of live animals and hypothermically preserved samples, indicating cellular function even under blood deprivation and hypothermic conditions.