FERNANDA DEGOBBI TENORIO QUIRINO DOS SANTOS LOPES
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- Smoking induces increased apoptosis in osteoblasts: changes in bone matrix organic components(2023) KOHLER, Julia Benini; SILVA, Alex Ferreira da; FARIAS, Walleson Alves; SAMPAIO, Barbara Fialho Carvalho; NEVES, Marco Aurelio Silveiro; LIMA, Leandro Gregorut; LOURENCO, Juliana Dias; MOREIRA, Alyne Riani; BARBOSA, Alexandre Povoa; TIBERIO, Iolanda de Fatima Lopes Calvo; TEODORO, Walcy Rosolia; LOPES, Fernanda Degobbi Tenorio Quirino dos SantosClinical studies demonstrate the impact of smoking on bone tissue fragility and higher incidence of fractures. However, it is not totally understood which physiological mechanisms could be involved in these events. Previously, we showed important changes in bone tissue components in experimental model of cigarette smoke (CS) exposure. CS exposure induces worsening in bone mineralization and a decrease in collagen type I deposition, leading to bone fragility. Considering that the majority of clinical studies described bone structural changes by radiographic images, in this study we performed analyses ""in situ"" using tissue samples from smokers, former smokers and non-smokers to better understand how the increase in inflammatory mediators induced by smoking exposure could interfere in bone cells activity leading bone structural changes. We observed increased levels of IL-1 beta, IL-6 and TNF-alpha in bone tissue homogenates with a concomitant increase in osteoblast apoptosis in smokers and former smokers compared with non-smokers. Histological changes in both smokers and former smokers were characterized by reduction in collagen type I. Only in smokers, it was observed decrease in trabecular area, suggesting increased bone resorption and increase in collagen type V. These results showed that osteoblasts apoptosis in association with increased bone resorption leads bone structural changes in smokers.
- Green Areas and PM2,5 exposure could be associated with Asthma and COPD?(2023) ALMEIDA, Francine Maria; MOREIRA, Tiana Carla Lopes; OLIVEIRA, Lucas Miranda; RIGHETTI, Renato Fraga; LOPES, Fernanda Degobbi Tenorio Quirino Santos; ALENCAR, Airlane P.; GOUVEIA, Nelson; MAUAD, Thais; LOTUFO, Paulo A.; BENSENOR, Isabela; SANTOS, Itamar Souza; TIBERIO, Iolanda Fatima Lopes Calvo
- Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma-Chronic Obstructive Pulmonary Disease Overlap (ACO)(2023) BARBOSA, Jessica Anastacia Silva; SILVA, Luana Laura Sales da; JOAO, Juliana Morelli Lopes Goncalves; CAMPOS, Elaine Cristina de; FUKUZAKI, Silvia; CAMARGO, Leandro do Nascimento; SANTOS, Tabata Maruyama dos; SANTOS, Henrique Tibucheski dos; BEZERRA, Suellen Karoline Moreira; SARAIVA-ROMANHOLO, Beatriz Mangueira; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; BONTURI, Camila Ramalho; OLIVA, Maria Luiza Vilela; LEICK, Edna Aparecida; RIGHETTI, Renato Fraga; TIBERIO, Iolanda de Fatima Lopes CalvoThe synthesized peptide derived from Enterolobium contortisiliquum (pep3-EcTI) has been associated with potent anti-inflammatory and antioxidant effects, and it may be a potential new treatment for asthma-COPD overlap-ACO). Purpose: To investigate the primary sequence effects of pep3-EcTI in an experimental ACO. BALB/c mice were divided into eight groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep3-EcTI (treated with inhibitor), ACO-DX (treated with dexamethasone), ACO-DX-pep3-EcTI (treated with dexamethasone and inhibitor), and SAL-pep3-EcTI (saline group treated with inhibitor). We evaluated the hyperresponsiveness to methacholine, exhaled nitric oxide, bronchoalveolar lavage fluid (BALF), mean linear intercept (Lm), inflammatory markers, tumor necrosis factor (TNF-alpha), interferon (IFN)), matrix metalloproteinases (MMPs), growth factor (TGF-beta), collagen fibers, the oxidative stress marker inducible nitric oxide synthase (iNOS), transcription factors, and the signaling pathway NF-kappa B in the airways (AW) and alveolar septa (AS). Statistical analysis was conducted using one-way ANOVA and t-tests, significant when p < 0.05. ACO caused alterations in the airways and alveolar septa. Compared with SAL, ACO-pep3-EcTI reversed the changes in the percentage of resistance of the respiratory system (%Rrs), the elastance of the respiratory system (%Ers), tissue resistance (%Gtis), tissue elastance (%Htis), airway resistance (%Raw), Lm, exhaled nitric oxide (ENO), lymphocytes, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, TNF-alpha, INF-gamma, MMP-12, transforming growth factor (TGF)-beta, collagen fibers, and iNOS. ACO-DX reversed the changes in %Rrs, %Ers, %Gtis, %Htis, %Raw, total cells, eosinophils, neutrophils, lymphocytes, macrophages, IL-1 beta, IL-6, IL-10, IL-13, IL-17, TNF-alpha, INF-gamma, MMP-12, TGF-beta, collagen fibers, and iNOS. ACO-DX-pep3-EcTI reversed the changes, as was also observed for the pep3-EcTI and the ACO-DX-pep3-EcTI. Significance: The pep3-EcTI was revealed to be a promising strategy for the treatment of ACO, asthma, and COPD.
- Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma-COPD Overlap (ACO) Model(2023) SILVA, Luana Laura Sales da; BARBOSA, Jessica Anastacia Silva; JOAO, Juliana Morelli Lopes Goncalves; FUKUZAKI, Silvia; CAMARGO, Leandro do Nascimento; SANTOS, Tabata Maruyama dos; CAMPOS, Elaine Cristina de; COSTA, Arthur Silva; SARAIVA-ROMANHOLO, Beatriz Mangueira; BEZERRA, Suellen Karoline Moreira; LOPES, Fernanda Tenorio Quirino dos Santos; BONTURI, Camila Ramalho; OLIVA, Maria Luiza Vilela; LEICK, Edna Aparecida; RIGHETTI, Renato Fraga; TIBERIO, Iolanda de Fatima Lopes Calvo(1) There are several patients with asthma-COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1 & beta;, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-& gamma;, TNF-& alpha;, MMP-9, MMP-12, TGF-& beta;, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-& kappa;B in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-& gamma;, TNF-& alpha;, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1 & beta;(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-& gamma;, MMP-12 (AS), TGF-& beta; (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model.
conferenceObject Plant Protease Inhibitors in Mice ACO Model: Comparison With Corticosteroid(2023) JOAO, J.; BARBOSA, J. A.; SILVA, L. L.; FUKUZAKI, S.; CAMARGO, L. D.; BEZERRA, S. K.; CAMPOS, E. C.; BONTURI, C.; LOPES, F. D.; OLIVA, M. V.; RIGHETTI, R. F.; TIBERIO, I. D.; LEICK, E. A.- The imbalance between subpopulations of regulatory T (TREG) Cells at different stages of chronic obstructive pulmonary disease (COPD)(2023) ALVES, Luan Henrique Vasconcelos; A, Juliana Tiyaki Ito; OLIVEIRA, Luana Mendonca De; TIBERIO, Iolanda Lopes Calvo; STELMACH, Rafael; SATO, Maria Notomi; ALMEIDA, Francine Maria; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos
conferenceObject Cholinergic System in the Exposure of Iron Particles in an Experimental Model of Chronic Allergic Inflammation(2023) TIBERIO, I. C.; SANTOS, T. M.; FUKUZAKI, S.; CAMPOS, E.; GALLI, T. T.; SILVA, L. L. S.; BARBOSA, J. A. S.; JOAO, J. M. L. G.; CAMARGO, L. D.; SARAIVA-ROMANHOLO, B. M.; CIRILLO, J. V. O.; BEZERRA, S. K. M.; PRADO, C. M.; MARTINS, M. A.; RIGHETTI, R. F.; LOPES, F. D.; LEICK, E. A.; SILVA, F. J. A.; REZENDE, B. G.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; LOTUFO, P. A.conferenceObject Preventive Effects of Inhibition of Thymic Stromal Lymphopoietin on Bronchial Hyperresponsiveness and Inflammatory Cellular Pattern in an Experimental Model of Chronic Allergic Pulmonary Inflammation Exacerbated by Lipopolysaccharide(2023) GRENCHESKI, E. A.; LOPES, B. Q. S.; CRUZ, E. L.; RIGHETTI, R. F.; SANTOS, T. M.; CAMARGO, L. D.; ALMEIDA, F.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; BEZERRA, S. K. M.; PIGATI, P. A.; LOPES, F. D.; LEICK, E. A.; TIBERIO, I. F. L. C.conferenceObject Particulate Matter Induces Lung Inflammation in Healthy Mice and Exacerbates Experimental Asthma Model Animals(2023) TIBERIO, I. C.; CAMPOS, E. C.; GALLI, T. T.; SANTOS, T. M.; FUKUZAKI, S.; CAMARGO, L. D.; HAMAGUCHI, S. S. S.; BEZERRA, S. K. M.; SILVA, F. J. A. da; REZENDE, B. G. de; LOPES, F. D. T. Q.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; LEICK, E. A.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; RIGHETTI, R. F.; LOTUFO, A.conferenceObject Effects of Exposure to Environmental Pollution From Iron Ore Pellets on Elastase-induced Lung Inflammation: Evaluation of the Effect of Reduced Acetylcholine Transporter Expression in Mice(2023) TIBERIO, I. C.; FUKUZAKI, S.; SANTOS, T. M.; CAMPOS, E. C.; SILVA, L. L. S.; BARBOSA, J. A. S.; JOAO, J. M. L. G.; CAMARGO, L. N.; BEZERRA, S. K. M.; CIRILLO, J. V. O.; PRADO, C. M.; SARAIVA-ROMANHOLO, B. M.; LEICK, E. A.; LOPES, F. D. T. Q.; RIGHETTI, R. F.; SILVA, F. J. A.; BENSENOR, I. J. M.; BOUROTTE, C. L. M.; MARTINS, M. A.; LOTUFO, P. A.