NATALIA GARCIA
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
21 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 21
- Involvement of miRNA expression may contribute for FoxO3a oncogenic role in uterine leiomyosarcoma(2018) GARCIA, Natalia; RICCI, Anamaria Ritti; ALMEIDA, Bruna Cristine de; ALMEIDA, Thais Gomes; CUNHA, Isabela Werneck; BARACAT, Edmund Chada; CARVALHO, Katia Candido
conferenceObject Melatonin action on luteal - granulosa cells in women with marital infertility undergoing in vitro fertilization(2016) MAGANHIN, Carla; CARVALHO, Katia; TURCO, Edson Lo; SERAFINI, Paulo; GARCIA, Natalia; CIPOLLA-NETTO, Jose; SIMOES, Manuel; BARACAT, Edmund; SOARES-JUNIOR, Jose Maria- Differential BMP4 and GREM1 protein expression in uterine leiomyosarcoma and leiomyoma(2013) GARCIA, Natalia; SOUZA, Faila Catarina; CUNHA, Isabela Werneck; SOARES, Fernando Augusto; MACIEL, Gustavo Arantes Rosa; BARACAT, Edmund Chada; CARVALHO, Katia Candido
- Targeting Hedgehog Pathway and DNA Methyltransferases in Uterine Leiomyosarcoma Cells(2021) GARCIA, Natalia; AL-HENDY, Ayman; BARACAT, Edmund C.; CARVALHO, Katia Candido; YANG, QiweiUterine leiomyosarcoma (LMS) is an aggressive tumor that presents a poor prognosis, high rates of recurrence, and metastasis. Because of its rarity, there is no information available concerning LMS molecular mechanisms of origin and development. Here, we assessed the expression profile of Hedgehog (HH) signaling pathway markers and the effects of their pharmacological inhibition on uterine smooth muscle (UTSM), leiomyoma, and LMS cells. Additionally, we also evaluated the effects of DNMTs inhibition on LMS cell behavior. Cell proliferation, migration and apoptosis rates were evaluated by MTT, Scratch, and Annexin V assays, respectively. RNA expression and protein levels were assessed by qRT-PCR and Western blot. We found that SMO and GLIs (1, 2, and 3) expression was upregulated in LMS cells, with increased nuclear levels of GLI proteins. Treatment with LDE225 (SMOi) and Gant61 (GLIi) resulted in a significant reduction in Glis protein levels in LMS (p < 0.05). Additionally, the expression of DNMT (1, 3a, and 3b), as well as GLI1 nuclear expression, was significantly decreased after treatment with HH inhibitor in LMS cells. Our results showed that blocking of SMO, GLI, and DNMTs is able to inhibit LMS proliferation, migration, and invasion. Importantly, the combination of those treatments exhibited a potentiated effect on LMS malignant features due to HH pathway deactivation.
- SHH pathway in uterine mesenchymal tumors(2014) GARCIA, Natalia; SOUZA, Faila C.; BOZZINI, Nilo; BAIOCCHI, Glauco; CUNHA, Isabela W.; SOARES, Fernando A.; BARACAT, Edmund C.; CARVALHO, Katia C.
- Differences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levels(2015) MARCONDES, Rodrigo R.; CARVALHO, Katia C.; DUARTE, Daniele C.; GARCIA, Natalia; AMARAL, Vinicius C.; SIMOES, Manuel J.; TURCO, Edson G. Lo; SOARES JR., Jose M.; BARACAT, Edmund C.; MACIEL, Gustavo A. R.Exposure to an excess of androgen or estrogen can induce changes in reproductive function in adult animals that resemble polycystic ovary syndrome in humans. However, considerable differences exist among several types of animal models. Little is known about the molecular features of steroidogenesis and folliculogenesis in the ovaries of rats exposed to different sex steroids as neonates. Here, we evaluated the impact of androgen and estrogen exposure on the ovaries of adult female rats during their neonatal period in the gene expression of Lhr and Cyp17a1, two key players of steroidogenesis. We also assessed hormone levels, folliculogenesis and the theca-interstitial cell population. The study was performed on the second postnatal day in thirty female Wistar rats that were sorted into the following three intervention groups: testosterone, estradiol and vehicle (control group). The animals were euthanized 90 days after birth. The main outcomes were hormone serum levels, ovary histomorphometry and gene expression of Lhr and Cyp17a1 as analyzed via quantitative real-time PCR. We found that exposure to excess testosterone in early life increased the LH and testosterone serum levels, the LH/FSH ratio, ovarian theca-interstitial area and gene expression of Lhr and Cyp17a1 in adult rats. Estrogen induced an increase in the ovarian theca-interstitial area, the secondary follicle population and gene expression of Lhr and Cyp17a1. All animals exposed to the sex steroids presented with closed vaginas. Our data suggest that testosterone resulted in more pronounced reproductive changes than did estrogen exposure. Our results might provide some insight into the role of different hormones on reproductive development and on the heterogeneity of clinical manifestations of conditions such as polycystic ovary syndrome.
conferenceObject GLI transcription factors involvement in leiomyoma genesis(2016) GARCIA, Natalia; ANJOS, Laura; BOZZINI, Nilo; MACIEL, Gustavo; BARACAT, Edmund; CARVALHO, KatiabookPart Sarcoma uterino(2014) CARVALHO, Kátia Candido; GARCIA, Natália; JúNIOR, José Maria Soares; MACIEL, Gustavo Arantes Rosa; BARACAT, Edmund ChadaconferenceObject Hypermethylation in BMP4 and GREM1 may be associated with uterine leiomyosarcoma development.(2018) GARCIA, Natalia; OLIVEIRA, Bianca Calsolari; FERREIRA, Kelly Pedrozo; BARACAT, Edmund Chada; CARVALHO, Katia CandidoconferenceObject Metastatic vulvar squamous cell carcinoma shows rs4986938 polymorphism in estrogen receptor beta.(2018) FERREIRA, Kelly Pedrozo; NARCISO, Henderson Junior; GARCIA, Natalia; BARACAT, Edmund Chada; CARVALHO, Katia Candido
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