EDSON DIAS MOREIRA
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- Baroreflex Sensitivity Impairment Is Associated With Cardiac Diastolic Dysfunction in Rats(2011) MOSTARDA, Cristiano; MORAES-SILVA, Ivana Cinthya; MOREIRA, Edson Dias; MEDEIROS, Alessandra; PIRATELLO, Aline Cristina; CONSOLIM-COLOMBO, Fernanda Marciano; CALDINI, Elia Garcia; BRUM, Patricia Chakur; KRIEGER, Eduardo Moacyr; IRIGOYEN, Maria ClaudiaBackground: Studies have shown that the autonomic dysfunction accompanied by impaired baroreflex sensitivity was associated with higher mortality. However, the influence of decreased baroreflex sensitivity on cardiac function, especially in diastolic function, is not well understood. This study evaluated the morpho-functional changes associated with baroreflex impairment induced by chronic sinoaortic denervation (SAD). Methods and Results: Animals were divided into sinoaortic denervation (SAD) and control (C) groups. Baroreflex sensitivity was evaluated by tachycardic and bradycardic responses, induced by vasoactive drugs. Cardiac function was studied by echocardiography and by left ventricle (LV) catheterization. LV collagen content and the expression of regulatory proteins involved in intracellular Ca(2+) homeostasis were quantified. Results showed higher LV mass in SAD versus C animals. Furthermore, an increase in deceleration time of E-wave in the SAD versus the C group (2.14 +/- 0.07 ms vs 1.78 +/- 0.03 ms) was observed. LV end-diastolic pressure was increased and the minimum dP/dt was decreased in the SAD versus the C group (12 +/- 1.5 mm Hg vs 5.3 +/- 0.2 mm Hg and 7,422 +/- 201 vs 4,999 +/- 345 mm Hg/s, respectively). SERCA/NCX ratio was lower in SAD than in control rats. The same was verified in SERCA/PLB ratio. Conclusions: The results suggest that baroreflex dysfunction is associated with cardiac diastolic dysfunction independently of the presence of other risk factors. (J Cardiac Fail 2011;17:519-525)
conferenceObject Chronic Absence of Baroreceptors Induces Left Ventricular Dysfunction and Reduced Coronary Blood Flow in Spontaneously Hypertensive Rats(2013) SOUZA, Pamella R.; MOREIRA, Edson D.; MOSTARDA, Cristiano; JORGE, Luciana; MONTEIRO-DE-MORAES, Wilson Max; GUIMARAES, Fabiana; OLIVEIRA, Edilamar Menezes; FLUES, Karin; SILVA, Maikon Barbosa; IRIGOYEN, Maria ClaudiaHemodynamic and cardiac morphological variables were measured in normotensive Wistar (N), intacts hypertensive (H) and SHR submitted to sinoaortic denervation (DH) to evaluate the chronic baroreceptor dysfunction on left ventricular (LV) functions and coronary blood flow. Blood (BP) and end-diastolic pressure (PD2) were obtained by direct quantification. The coronary blood flow was measured by the infusion of colored microspheres. The ratio of left ventricular weight to body weight, natriuretic atrial peptide (ANP), alpha-skeletal actin (α-skelectal), alpha and beta myosin heavy chain (α-MHC, β-MHC) genes mRNA expressions were evaluated by real time PCR to assess ventricular hypertrophy. The results were reported as means ± SEM. DH rats did not change BP values compared to H. PD2 of DH (9.91±1.78) was higher than H group (4.30±0.53) while an inverse result regarding coronary blood flow was observed (0.94±0.15 vs 1.80±0.25, respectively). The LV adaptations in DH were higher than in H (2.98 ±0.07 vs 2.15±0.05), as well as the expression of ANP (3.35±0.19 vs 2.39 ± 0.74), β-MHC (3.36±0.18 vs 2.39 ± 0.37). The α-MHC genes expression also were decreased (0.62±0.10 vs 0.79±0.10). Therefore, the chronic absence of baroreflex control by sinoaortic denervation induces left ventricular dysfunction and reduced coronary blood flow in SHR.article 9 Citação(ões) na Scopus Baroreflex deficiency induces additional impairment of vagal tone, diastolic function and calcium handling proteins after myocardial infarction(2014) MOSTARDA, Cristiano; RODRIGUES, Bruno; MEDEIROS, Alessandra; MOREIRA, Edson D.; MORAES-SILVA, Ivana C.; BRUM, Patricia C.; ANGELIS, Katia De; IRIGOYEN, Maria-ClaudiaBaroreflex dysfunction has been considered an important mortality predictor after myocardial infarction (MI). However, the impact of baroreflex deficiency prior to MI on tonic autonomic control and cardiac function, and on the profile of proteins associated with intracellular calcium handling has not yet been studied. The aim of the present study was to analyze how the impairment of baroreflex induced by sinoaortic denervation (SAD) prior to MI in rats affects the tonic autonomic control, ventricular function and cardiomyocyte calcium handling proteins. After 15 days of following or SAD surgery, rats underwent MI. Echocardiographic, hemodynamic, autonomic and molecular evaluations were performed 90 days after MI. Baroreflex impairment led to additional damage on: left ventricular remodeling, diastolic function, vagal tonus and intrinsic heart rate after MI. The loss of vagal component of the arterial baroreflex and vagal tonus were correlated with changes in the cardiac proteins involved in intracellular calcium homeostasis. Furthermore, additional increase in sodium calcium exchanger expression levels was associated with impaired diastolic function in experimental animals. Our findings strongly suggest that previous arterial baroreflex deficiency may induce additional impairment of vagal tonus, which was associated with calcium handling proteins abnormalities, probably triggering ventricular diastolic dysfunction after MI in rats.- Cardiac and pulmonary arterial remodeling after sinoaortic denervation in normotensive rats(2012) FLUES, K.; MORAES-SILVA, I. C.; MOSTARDA, C.; SOUZA, P. R. M.; DINIZ, G. P.; MOREIRA, E. D.; PIRATELLO, A. C.; CHAVES, M. L. Barreto; ANGELIS, K. De; SALEMI, Vera Maria Cury; IRIGOYEN, M. C.; CALDINI, E. G.Blood pressure variability (BPV) and baroreflex dysfunction may contribute to end-organ damage process. We investigated the effects of baroreceptor deficit (10 weeks after sinoaortic denervation - SAD) on hemodynamic alterations, cardiac and pulmonary remodeling. Cardiac function and morphology of male Wistar intact rats (C) and SAD rats (SAD) (n = 8/group) were assessed by echocardiography and collagen quantification. BP was directly recorded. Ventricular hypertrophy was quantified by the ratio of left ventricular weight (LVW) and right ventricular weight (RVW) to body weight (BW). BPV was quantified in the time and frequency domains. The atrial natriuretic peptide (ANP), alpha-skeletal actin (alpha-skelectal), collagen type I and type III genes mRNA expression were evaluated by RT-PCR. SAD did not change BP, but increased BPV (11 +/- 0.49 vs. 5 +/- 0.3 mm Hg). As expected, baroreflex was reduced in SAD. Pulmonary artery acceleration time was reduced in SAD. In addition, SAD impaired diastolic function in both LV (6.8 +/- 0.26 vs. 5.02 +/- 0.21 mm Hg) and RV (5.1 +/- 0.21 vs. 4.2 +/- 0.12 mm Hg). SAD increased LVW/BW in 9% and RVW/BW in 20%, and augmented total collagen (3.8-fold in LV, 2.7-fold in RV, and 3.35-fold in pulmonary artery). Also, SAD increased type I (similar to 6-fold) and III (similar to 5-fold) collagen gene expression. Denervation increased ANP expression in LV (75%), in RV (74%) and increased a-skelectal expression in LV (300%) and in RV (546%). Baroreflex function impairment by SAD, despite not changing BP, induced important adjustments in cardiac structure and pulmonary hypertension. These changes may indicate that isolated baroreflex dysfunction can modulate target tissue damage.
- Preventive role of exercise training in autonomic, hemodynamic, and metabolic parameters in rats under high risk of metabolic syndrome development(2013) MORAES-SILVA, Ivana Cinthya; MOSTARDA, Cristiano; MOREIRA, Edson Dias; SILVA, Kleiton Augusto Santos; SANTOS, Fernando dos; ANGELIS, Katia de; FARAH, Vera de Moura Azevedo; IRIGOYEN, Maria ClaudiaPreventive role of exercise training in autonomic, hemodynamic, and metabolic parameters in rats under high risk of metabolic syndrome development. J Appl Physiol 114: 786-791, 2013. First published January 17, 2013; doi:10.1152/japplphysiol.00586.2012.-High fructose consumption contributes to metabolic syndrome incidence, whereas exercise training promotes several beneficial adaptations. In this study, we demonstrated the preventive role of exercise training in the metabolic syndrome derangements in a rat model. Wistar rats receiving fructose overload in drinking water (100 g/l) were concomitantly trained on a treadmill (FT) or kept sedentary (F) for 10 wk. Control rats treated with normal water were also submitted to exercise training (CT) or sedentarism (C). Metabolic evaluations consisted of the Lee index and glycemia and insulin tolerance test (kITT). Blood pressure (BP) was directly measured, whereas heart rate (HR) and BP variabilities were evaluated in time and frequency domains. Renal sympathetic nerve activity was also recorded. F rats presented significant alterations compared with all the other groups in insulin resistance (in mg.dl(-1).min(-1): F: 3.4 +/- 0.2; C: 4.7 +/- 0.2; CT: 5.0 +/- 0.5 FT: 4.6 +/- 0.4), mean BP (in mmHG: F: 117 +/- 2; C: 100 +/- 2; CT: 98 +/- 2; FT: 105 +/- 2), and Lee index (in g/mm: F = 0.31 +/- 0.001; C = 0.29 +/- 0.001; CT = 0.27 +/- 0.002; FT = 0.28 +/- 0.002), confirming the metabolic syndrome diagnosis. Exercise training blunted all these derangements. Additionally, FS group presented autonomic dysfunction in relation to the others, as seen by an similar to 50% decrease in baroreflex sensitivity and 24% in HR variability, and increases in sympathovagal balance (140%) and in renal sympathetic nerve activity (45%). These impairments were not observed in FT group, as well as in C and CT. Correlation analysis showed that both Lee index and kITT were associated with vagal impairment caused by fructose. Therefore, exercise training plays a preventive role in both autonomic and hemodynamic alterations related to the excessive fructose consumption.
conferenceObject Effect of sinoaortic denervation on blood pressure and heart rate of hypertensive and control rats monitored by telemetry(2013) SANTOS, Fernando; MOREIRA, Edson Dias; MOSTARDA, Cristiano Teixeira; BARBOSA, Maikon; IRIGOYEN, Maria ClaudiaThe aim of this study was to evaluate the effects of sinoaortic denervation (SAD) on systolic blood pressure (SBP) and heart rate (HR), and possible changes in circadian cycle. Wistar (C) and spontaneously hypertensive (H) male rats were submitted to SAD and monitored for two weeks by radio transmitter implanted directly into the abdominal aorta. The acute period (48hs) after SAD procedure was characterized by SBP increase in C (39%) and in H (17%). Surprisingly, a decrease in SBP in H (12%) below baseline levels was observed nine hours after the surgery and was maintained during the next 48 hours. In contrast, SBP in C was still 14% above the baseline. C HR was unchanged, while in H it was 29% increased in the first 48 hours. After two weeks there were no significant differences in SBP and HR in C while in H a 14% reduction in SBP was observed in comparison to the baseline. In this group HR remained 3% higher. We noticed that in H group the behavior of SBP and HR did not change through circadian cycle phases. After SAD the behavior of SPB was still unchanged in H group while HR presented an increase of 6% during the active period. However, SBP behavior in the C was different during light/dark periods, both before and after SAD, with an increase in HR in active period before (8%) and after (11%) SAD. These data suggest that SAD is able to reduce SBP of H and to change the behavior of HR in hypertensive and control rats.conferenceObject Autonomic and metabolic changes induced by fructose overload: role of baroreflex(2014) SANTOS, Fernando; MOREIRA, Edson; SARTORI, Michelle; IRIGOYEN, Maria- Previous Exercise Training Has a Beneficial Effect on Renal and Cardiovascular Function in a Model of Diabetes(2012) SILVA, Kleiton Augusto dos Santos; LUIZ, Rafael da Silva; RAMPASO, Rodolfo Rosseto; ABREU, Nayda Parisio de; MOREIRA, Edson Dias; MOSTARDA, Cristiano Teixeira; ANGELIS, Katia De; TEIXEIRA, Vicente de Paulo Castro; IRIGOYEN, Maria Claudia; SCHOR, NestorExercise training (ET) is an important intervention for chronic diseases such as diabetes mellitus (DM). However, it is not known whether previous exercise training intervention alters the physiological and medical complications of these diseases. We investigated the effects of previous ET on the progression of renal disease and cardiovascular autonomic control in rats with streptozotocin (STZ)-induced DM. Male Wistar rats were divided into five groups. All groups were followed for 15 weeks. Trained control and trained diabetic rats underwent 10 weeks of exercise training, whereas previously trained diabetic rats underwent 14 weeks of exercise training. Renal function, proteinuria, renal sympathetic nerve activity (RSNA) and the echocardiographic parameters autonomic modulation and baroreflex sensitivity (BRS) were evaluated. In the previously trained group, the urinary albumin/creatinine ratio was reduced compared with the sedentary diabetic and trained diabetic groups (p < 0.05). Additionally, RSNA was normalized in the trained diabetic and previously trained diabetic animals (p < 0.05). The ejection fraction was increased in the previously trained diabetic animals compared with the diabetic and trained diabetic groups (p < 0.05), and the myocardial performance index was improved in the previously trained diabetic group compared with the diabetic and trained diabetic groups (p < 0.05). In addition, the previously trained rats had improved heart rate variability and BRS in the tachycardic response and bradycardic response in relation to the diabetic group (p < 0.05). This study demonstrates that previous ET improves the functional damage that affects DM. Additionally, our findings suggest that the development of renal and cardiac dysfunction can be minimized by 4 weeks of ET before the induction of DM by STZ.