CONSUELO JUNQUEIRA RODRIGUES
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Ortopediae Traumatologia, Faculdade de Medicina - Docente
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina - Líder
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- Study of 1550nm Erbium Glass Laser Fractional non-ablative treatment of photoaging: comparative clinical effects, histopathology, electron microscopy and immunohistochemistry(2023) SICA, Regia Celli Patriota de; RODRIGUES, Consuelo J.; MARIA, Durvanei Augusto; CUCE, Luis CarlosWe, the Editors and Publisher of the Journal of Cosmetic and Laser Therapy have retracted the following article:Regia Celli Patriota de Sica, Consuelo J. Rodrigues, Durvanei Augusto Maria & Luis Carlos Cuce (2016) Study of 1550nm Erbium Glass Laser Fractional non-ablative treatment of photoaging: Comparative clinical effects, histopathology, electron microscopy and immunohistochemistry, Journal of Cosmetic and Laser Therapy, DOI: 10.1080/14764172.2016.1191647Since publication of the accepted author version, authors have not responded to requests to submit corrections and approve proofs, preventing the final publication of the Version of Record (VoR). Authors have also not provided completed copyright forms.We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as 'Retracted'.
article 19 Citação(ões) na Scopus Intense pulsed light in photoaging: a clinical, histopathological and immunohistochemical evaluation(2011) PATRIOTA, Regia Celli Ribeiro; RODRIGUES, Consuelo Junqueira; CUCE, Luiz CarlosBACKGROUND: Intense pulsed light has been used in the treatment of photoaging without a full understanding of its mechanism of action. OBJECTIVE: To study the effect of intense pulsed light on photoaging and on the skin immune response by means of a clinical and histopathological study, evaluating Langerhans cells (CD1), expression of intercellular adhesion molecule, of CD4 and CD8 lymphocytes and quantification of collagen and elastic fibers. METHODS: In 2006 a total of 26 patients, aged 40 to 65 years, with phototypes II to III (Fitzpatrick scale), were treated for photoaging using intense pulsed light in five sessions with a monthly interval. All the patients were subjected to histological and immunohistochemical evaluation 6 months after treatment. RESULTS: At the end of the treatment clinical improvement was observed in 76.92% of cases. This improvement was associated to a significant increase of collagen (51.33%) and elastic (44.13%) fibers. Intense pulsed light treatment led to a reduction of CD4 lymphocytes and did not alter the amount of CD8 lymphocytes. It also led to a significant increase of small, nonectatic blood vessels, positive intercellular adhesion molecule. CONCLUSION: Facial treatment with intense pulsed light promoted major clinical improvement that was confirmed by histological examination of the skin. This technique is a good treatment option for skin photoaging because it is non-ablative, safe and effective.- Are femoroacetabular impingement tomographic angles associated with the histological assessment of labral tears? A cadaveric study(2018) EJNISMAN, Leandro; DOMB, Benjamin G.; SOUZA, Felipe; JUNQUEIRA, Consuelo; VICENTE, Jose Ricardo Negreiros; CROCI, Alberto TesconiPurpose This study sought to investigate the association between tomographic femoroacetabular impingement (FAI) angles and histologically evaluated labral tears. The authors hypothesized that cadavers presenting with cam and pincer morphologies would present a higher prevalence of acetabular labral tears. Methods Twenty fresh cadavers were submitted to computed tomography. Standard FAI angles were measured, including the alpha angle, femoral version, acetabular version, Tonnis angle and center-edge angle. A cam lesion was defined as an alpha angle greater than 50 degrees. A pincer lesion was defined as a center-edge angle greater than 40 degrees, a Tonnis angle less than 0 degrees or acetabular version less than 0 degrees. After dissection, three fragments of each acetabulum, corresponding to the antero-superior, superior and postero-superior acetabular rim, were obtained. These fragments were submitted to routine histological preparation. Each slide was evaluated for possible labral tears. Tears were classified according to their Seldes type. Results The mean age of the cadavers was 50.2 years (SD: 7.4; 13 males). Sixteen (80%) of the cadavers had a cam lesion, and eight cadavers (40%) had a pincer lesion. Histologically, 16 (80%) of the cadavers had a labral tear in at least one region. According to the Seldes classification, 60.7% and 28.6% of these labral tears were type 1 and type 2, respectively. A mixed type of labral tear (10.7%), which represented a new form of Seldes tear, was described. Cadavers with a labral tear had significantly higher alpha angles than other cadavers (53.29 degrees vs 49.33 degrees, p = 0.01). Pincer lesions were not associated with labral tears. We found no association between pincer or cam lesions and Seldes classification. Conclusion Cadavers presenting with higher alpha angles had a higher incidence of labral tears. No association was found between FAI and Seldes classification. Clinical relevance This study demonstrated a high prevalence of FAI abnormalities associated with histological alterations in a cadaveric sample. Joint damage may be present in the early stages of FAI.
- Study of 1550-nm Erbium glass laser fractional non-ablative treatment of photoaging: Comparative clinical effects, histopathology, electron microscopy, and immunohistochemistry(2016) SICA, Regia Celli Patriota de; RODRIGUES, Consuelo J.; MARIA, Durvanei Augusto; CUCE, Luis CarlosBackground: Non-ablative fractional lasers have been effectively used in skin rejuvenation. Objectives: This study evaluates the efficacy of 1550-nm Erbium glass laser for facial rejuvenation through the correlation of clinical evaluation and histopathology, immunohistochemistry, and electron microscopy analysis. Methods: Fifteen subjects (average age: 56.4 years, skin types: I-III) with mild-to-moderate photodamage were submitted to biopsies and 3 facial treatments. Data from the photo assessments and the clinical improvement were analyzed 4 months after the treatments. The biopsy skins were fixed in neutral buffered formalin before being embedded in paraffin, and stained with hematoxylin and eosin. The histomorphometric quantification of collagen and elastic fibers; intercellular adhesion molecule 1 expression by immunohistochemistry; and analysis of cell cycle phases, the electrical potential of the mitochondrial, and interleukin (IL)-1, CD34, transforming growth factor (TGF)-beta, and caspase-3 expression by flow cytometry were analyzed. Results: After 4 months of treatment, collagen fibers had increased by 6.68%, and intercellular adhesion molecule 1 (ICAM-1) had increased by 4.47% in vessel area. Significantly enhanced IL-1 and TGF-beta receptor expressions were identified after treatment. Proliferative responses and non-apoptosis-dependent caspase-3 activity were both observed in the cell after dermal treatment. Conclusion: The histopathology, immunohistochemistry, and electron microscopy showed an improvement compatible to the clinical effectiveness after 4 months.
- Comparison between the effects of platelet-rich plasma and bone marrow concentrate on defect consolidation in the rabbit tibia(2011) BATISTA, Marco Antonio; LEIVAS, Tomaz Puga; RODRIGUES, Consuelo Junqueira; ARENAS, Gessica Cantadori Funes; BELITARDO, Donizeti Rodrigues; GUARNIERO, RobertoOBJECTIVE: To perform a comparative analysis of the effects of platelet-rich plasma and centrifuged bone marrow aspirate on the induction of bone healing in rabbits. METHOD: Twenty adult, male New Zealand rabbits were randomly separated into two equal groups, and surgery was performed to create a bone defect (a cortical orifice 3.3 mm in diameter) in the proximal metaphysis of each rabbit's right tibia. In the first group, platelet-rich plasma was implanted in combination with beta-tricalcium phosphate (platelet-rich plasma group), and in the second group, centrifuged bone marrow in combination with beta-tricalcium phosphate (centrifuged bone marrow group) was implanted. After a period of four weeks, the animals were euthanized, and the tibias were evaluated using digital radiography, computed tomography, and histomorphometry. RESULTS: Seven samples from each group were evaluated. The radiographic evaluation confirmed the absence of fractures in the postoperative limb and identified whether bone consolidation had occurred. The tomographic evaluation revealed a greater amount of consolidation and the formation of a greater cortical bone thickness in the platelet-rich plasma group. The histomorphometry revealed a greater bone density in the platelet-rich plasma group compared with the centrifuged bone marrow group. CONCLUSION: After four weeks, the platelet-rich plasma promoted a greater amount of bone consolidation than the bone marrow aspirate concentrate.
- Quantitative evaluation of collagen and elastic fibers after intense pulsed light treatment of mouse skin(2018) FAUCZ, Luciana L.; WILL, Sonia E.; RODRIGUES, Consuelo J.; HESSE, Henrique; MORAES, Angelina C.; MARIA, Durvanei A.Background and ObjectiveThe aging of human skin includes intrinsic aging and photo-aging, which are characterized by a decrease in collagen and the deposition of abnormal elastic fibers. Intense pulsed light (IPL) sources are widely used in medicine to treat various cosmetic problems, including photo-damaged skin. Few studies have examined the microscopic changes produced by IPL. The objective of this study was to quantitatively evaluate the effects of IPL on collagen and elastic fibers in mice. Materials and MethodsForty female BALB/c mice were divided into four subgroups. Group 1 was the control group (n=10), and groups 2, 3, and 4 were treatment groups (n=10 in each group). Group 2 received one treatment, group 3 received two treatments, and group 4 received three treatments every 2 weeks. Skin tissue was obtained from irradiated areas 24 hours after the last treatment in each mouse. Collagen fibers were identified using the picrosirius red method. Elastic fibers were marked by Weigert-oxone stain. All samples were analyzed and quantified by a light microscope using analyzer system images. ResultsGroup 4, which received three IPL treatments, showed significant quantitative increases in both collagen fibers (P<0.05) and elastic fibers (P<0.01). Collagen fibers demonstrated a better parallel distribution in relation to the epidermis. ConclusionIPL treatment significantly increased the number of collagen and elastic fibers within the dermis and improved the parallel distribution of collagen fibers in relation to the epidermis. These results were evident after three IPL treatments. Lasers Surg. Med. 50:644-650, 2018. (c) 2018 Wiley Periodicals, Inc.
- Parathyroid gland anatomical distribution and relation to anthropometric and demographic parameters: a cadaveric study(2011) HOJAIJ, Flavio; VANDERLEI, Felipe; PLOPPER, Caio; RODRIGUES, Consuelo Junqueira; JACOMO, Alfredo; CERNEA, Claudio; OLIVEIRA, Leonardo; MARCHI, Luis; BRANDAO, LenineParathyroid glands play an important role in controlling calcium levels, which influence muscular contraction and neurotransmission. The number of variants, localization and ectopic positions make these glands tricky during surgical exploration. Detailed anatomical knowledge of these glands is fundamental to avoid postsurgical hypoparathyroidism, such as failures during thyroidectomy and parathyroid procedures. The purposes of this work were to study and report practical knowledge for surgeons in order to localize the glands. Dissections were performed on 56 cadavers. Gland identity was confirmed by histological study. Also, mediastinal tissue and the carotid sheath were treated with Carnoy's solution to identify ectopic glands. The thyroid gland was divided and sliced to identify parathyroid glands in the parenchymal and subcapsular space. Four or more parathyroid glands were found in 89.3% of the studied specimens. Mean gland weight was 33.1 mg, and its mean measurements were 6.7 x 3.9 x 2.0 mm. In more than 90% of the cases there was a correlation with the inferior laryngeal nerve and the parathyroid glands: the upper glands were located in medial positions, and the lower ones were found to be located laterally. In 42.8% of cases at least one ectopic gland was observed. The main ectopic regions were the mediastinum and thymus (19.6%), thyroid subcapsular space (12.5%) and thyroid parenchyma (5.4%). Quantity, gland characteristics and location were not influenced by anthropometric and demographic parameters. Here we show the high incidence of parathyroid glands positioned at ""abnormal"" locations, and as a controversial topic in endocrine surgery, this matter must be continuously studied and reported in the literature.
conferenceObject ENGRAFTMENT OF HUMAN ADIPOSE DERIVED STEM CELLS DELIVERED IN A HYALURONIC ACID PREPARATION IN MICE(2012) DIETRICH, Isa; COCHET, Olivia; VILLAGEOIS, Phi; RODRIGUES, Consuelo JunqueiraPurpose: To evaluate the implant of human adipose-derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected subcutaneously in athymic mice. Methods: Control implants: HA plus culture media was injected in the subcutaneous of the left subscapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With 8 weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxylin–eosin staining and immunofluorescence using anti-human vimentin and anti-von Willebrand factor antibodies. Results–Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxylin–eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants-A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxylin-eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. Conclusion: The delivery of ADSC in preparations of hyaluronic acid assured cells engraftment at the site of injection.- Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice(2012) DIETRICH, Isa; COCHET, Olivia; VILLAGEOIS, Phi; RODRIGUES, Consuelo JunqueiraPURPOSE: To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice. METHODS: Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies. RESULTS: Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin-eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. CONCLUSION: The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.
bookPart Nômina Anatômica(2017) JúNIOR, Aldo Junqueira Rodrigues; RODRIGUES, Consuelo Junqueira; JáCOMO, Alfredo Luis; OLIVEIRA, Rafael Garcia de