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https://observatorio.fm.usp.br/handle/OPI/2176
Title: | Bone marrow mononuclear cell therapy in experimental allergic asthma: Intratracheal versus intravenous administration |
Authors: | ABREU, Soraia C.; ANTUNES, Mariana A.; MARON-GUTIERREZ, Tatiana; CRUZ, Fernanda F.; ORNELLAS, Debora S.; SILVA, Adriana L.; DIAZ, Bruno L.; AB'SABER, Alexandre M.; CAPELOZZI, Vera L.; XISTO, Debora G.; MORALES, Marcelo M.; ROCCO, Patricia R. M. |
Citation: | RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, v.185, n.3, p.615-624, 2013 |
Abstract: | We hypothesized that the route of administration would impact the beneficial effects of bone marrow-derived mononuclear cell (BMDMC) therapy on the remodelling process of asthma. C57BL/6 mice were randomly assigned to two main groups. In the OVA group, mice were sensitized and challenged with ovalbumin, while the control group received saline using the same protocol. Twenty-four hours before the first challenge, control and OVA animals were further randomized into three subgroups to receive saline (SAL), BMDMCs intravenously (2 x 10(6)), or BMDMCs intratracheally (2 x 10(6)). The following changes were induced by BMDMC therapy in OVA mice regardless of administration route: reduction in resistive and viscoelastic pressures, static elastance, eosinophil infiltration, collagen fibre content in airways and lung parenchyma; and reduction in the levels of interleukin (IL)-4, IL-13, transforming growth factor-beta and vascular endothelial growth factor. In conclusion, BMDMC modulated inflammatory and remodelling processes regardless of administration route in this experimental model of allergic asthma. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - ODS/03 |
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