Cell Therapy for Fibrotic Interstitial Pulmonary Disease: Experimental Study
Carregando...
Citações na Scopus
11
Tipo de produção
article
Data de publicação
2011
Editora
WILEY-BLACKWELL
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Autores
CABRAL, Rosa M.
BRANCO, Erika
RIZZO, Marcia Dos S.
FERREIRA, Guilherme J.
GREGORES, Guilherme B.
SAMOTO, Vivian Y.
STOPIGLIA, Angelo J.
MAIORKA, Paulo C.
FIORETTO, Emerson T.
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
MICROSCOPY RESEARCH AND TECHNIQUE, v.74, n.10, p.957-962, 2011
Resumo
Parte superior do formulario Digite um texto ou endereco de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced-disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High-resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90-days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180-days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre-existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech. 74:957-962, 2011. (C) 2011 Wiley-Liss, Inc.
Palavras-chave
fibrotic interstitial pulmonary disease, cell therapy, histological changes in lung
Referências
- Rojas M, 2005, AM J RESP CELL MOL, V33, P145, DOI 10.1165/rcmb.2004-0330OC
- Ryu JH, 2007, MAYO CLIN PROC, V82, P976
- Moeller A, 2008, INT J BIOCHEM CELL B, V40, P362, DOI 10.1016/j.biocel.2007.08.011
- Khubchandani KR, 2001, FASEB J, V15, P59, DOI 10.1096/fj.00-0318rev
- Ortiz LA, 2003, P NATL ACAD SCI USA, V100, P8407, DOI 10.1073/pnas.1432929100
- Moodley YP, 2004, J PATHOL, V202, P486, DOI 10.1002/path.1531
- Kotton DN, 2001, DEVELOPMENT, V128, P5181
- Bellini A, 2007, LAB INVEST, V87, P858, DOI 10.1038/labinvest.3700654
- Gross TJ, 2001, NEW ENGL J MED, V345, P517, DOI 10.1056/NEJMra003200
- Lynch DA, 2005, AM J RESP CRIT CARE, V172, P488, DOI 10.1164/rccm.200412-1756OC
- Hashimoto N, 2004, J CLIN INVEST, V113, P243, DOI 10.1172/JCI200418847
- BALAZS G, 1994, RADIOLOGY, V191, P269
- BEREND N, 1985, AM REV RESPIR DIS, V132, P582
- Coletta Ester Nei Aparecida Martins, 2003, J. Pneumologia, V29, P371, DOI 10.1590/S0102-35862003000600009
- Cottin V, 2004, REV MAL RESPIR, V21, P299
- Dooner M, 2004, BLOOD CELL MOL DIS, V32, P47, DOI 10.1016/j.bcmd.2003.09.014
- Dunsmore SE, 2004, J CLIN INVEST, V113, P180, DOI 10.1172/JCI200420782
- Grande N., 1998, SCANNING MICROSCOPY, V12, P487
- Kasper M, 1996, HISTOCHEM CELL BIOL, V105, P221, DOI 10.1007/BF01462295
- KOTTON DN, 2008, CELL TISSUE RES, V331, P45
- Kotton DN, 2004, EXP HEMATOL, V32, P340, DOI 10.1016/j.exphem.2004.01.009
- Liem NT, 2008, JPN J INFECT DIS, V61, P157
- Loebinger MR, 2007, CHEST, V132, P279, DOI 10.1378/chest.06-2751
- MANOURY B, 2005, RESP RES, V6, P1465
- MCCULLOUGH B, 1978, J CLIN INVEST, V61, P79, DOI 10.1172/JCI108928
- Parra ER, 2005, LUNG, V183, P291, DOI 10.1007/s00408-004-2542-z
- Serra André Peres e, 2006, Clinics, V61, P497, DOI 10.1590/S1807-59322006000600002