Quantitative evaluation of collagen and elastic fibers after intense pulsed light treatment of mouse skin

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art_FAUCZ_Quantitative_evaluation_of_collagen_and_elastic_fibers_after_2018.PDF (1.53 MB)
Citações na Scopus
16
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
FAUCZ, Luciana L.
WILL, Sonia E.
HESSE, Henrique
MORAES, Angelina C.
MARIA, Durvanei A.
Citação
LASERS IN SURGERY AND MEDICINE, v.50, n.6, p.644-650, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background and ObjectiveThe aging of human skin includes intrinsic aging and photo-aging, which are characterized by a decrease in collagen and the deposition of abnormal elastic fibers. Intense pulsed light (IPL) sources are widely used in medicine to treat various cosmetic problems, including photo-damaged skin. Few studies have examined the microscopic changes produced by IPL. The objective of this study was to quantitatively evaluate the effects of IPL on collagen and elastic fibers in mice. Materials and MethodsForty female BALB/c mice were divided into four subgroups. Group 1 was the control group (n=10), and groups 2, 3, and 4 were treatment groups (n=10 in each group). Group 2 received one treatment, group 3 received two treatments, and group 4 received three treatments every 2 weeks. Skin tissue was obtained from irradiated areas 24 hours after the last treatment in each mouse. Collagen fibers were identified using the picrosirius red method. Elastic fibers were marked by Weigert-oxone stain. All samples were analyzed and quantified by a light microscope using analyzer system images. ResultsGroup 4, which received three IPL treatments, showed significant quantitative increases in both collagen fibers (P<0.05) and elastic fibers (P<0.01). Collagen fibers demonstrated a better parallel distribution in relation to the epidermis. ConclusionIPL treatment significantly increased the number of collagen and elastic fibers within the dermis and improved the parallel distribution of collagen fibers in relation to the epidermis. These results were evident after three IPL treatments. Lasers Surg. Med. 50:644-650, 2018. (c) 2018 Wiley Periodicals, Inc.
Palavras-chave
intense pulsed light, collagen fibers, elastic fibers, skin, mice
URI
https://observatorio.fm.usp.br/handle/OPI/28187
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MOT