Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

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art_DURIGON_Aerobic_Exercise_Protects_from_Pseudomonas_aeruginosaInduced_Pneumonia_in_2018.PDF (1.59 MB)
Citações na Scopus
22
Tipo de produção
article
Data de publicação
2018
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
KARGER
Autores
DURIGON, Thomas Stravinskas
MACKENZIE, BreAnne
OLIVEIRA-JUNIOR, Manoel Carneiro
SANTOS-DIAS, Alana
ANGELIS, Katia De
MALFITANO, Christiano
PALMA, Renata Kelly da
GUERRA, Juliana Moreno
Citação
JOURNAL OF INNATE IMMUNITY, v.10, n.4, p.279-290, 2018
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 x 10(4) colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1 beta (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-alpha (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-kappa B expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Preincubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-alpha (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/ NF-.B, and redox signaling. (C) 2018 S. Karger AG, Basel
Palavras-chave
Exercise immunology, Pseudomonas, Cytokines, Elderly, Physical training
URI
https://observatorio.fm.usp.br/handle/OPI/29587
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - LIM/59
Artigos e Materiais de Revistas Científicas - ODS/03