Protein disulfide isomerase plasma levels in healthy humans reveal proteomic signatures involved in contrasting endothelial phenotypes

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art_OLIVEIRA_Protein_disulfide_isomerase_plasma_levels_in_healthy_humans_2019.PDF (11.36 MB)
Citações na Scopus
21
Tipo de produção
article
Data de publicação
2019
Editora
ELSEVIER SCIENCE BV
DOI
Indexadores
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Autores
GARCIA-ROSA, Sheila
SACHETTO, Ana Teresa Azevedo
MORETTI, Ana Iochabel Soares
SILVA, Nathalia Tenguan
MARTINS-DE-SOUZA, Daniel
SANTORO, Marcelo Larami
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
REDOX BIOLOGY, v.22, article ID UNSP 101142, 14p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Redox-related plasma proteins are candidate reporters of protein signatures associated with endothelial structure/function. Thiol-proteins from protein disulfide isomerase (PDI) family are unexplored in this context. Here, we investigate the occurrence and physiological significance of a circulating pool of PDI in healthy humans. We validated an assay for detecting PDI in plasma of healthy individuals. Our results indicate high inter-individual (median = 330 pg/mL) but low intra-individual variability over time and repeated measurements. Remarkably, plasma PDI levels could discriminate between distinct plasma proteome signatures, with PDI-rich ( > median) plasma differentially expressing proteins related to cell differentiation, protein processing, housekeeping functions and others, while PDI-poor plasma differentially displayed proteins associated with coagulation, inflammatory responses and immunoactivation. Platelet function was similar among individuals with PDI-rich vs. PDI-poor plasma. Remarkably, such protein signatures closely correlated with endothelial function and phenotype, since cultured endothelial cells incubated with PDI-poor or PDI-rich plasma recapitulated gene expression and secretome patterns in line with their corresponding plasma signatures. Furthermore, such signatures translated into functional responses, with PDI-poor plasma promoting impairment of endothelial adhesion to fibronectin and a disturbed pattern of wound-associated migration and recovery area. Patients with cardiovascular events had lower PDI levels vs. healthy individuals. This is the first study describing PDI levels as reporters of specific plasma proteome signatures directly promoting contrasting endothelial phenotypes and functional responses.
Palavras-chave
Protein disulfide isomerase, Plasma proteome, Endothelial cells, Plasma protein signatures, Thiol proteins
URI
https://observatorio.fm.usp.br/handle/OPI/31947
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Coleções
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - FM/MCP
Artigos e Materiais de Revistas Científicas - LIM/13
Artigos e Materiais de Revistas Científicas - LIM/64