Directional Topography Influences Adipose Mesenchymal Stromal Cell Plasticity: Prospects for Tissue Engineering and Fibrosis
Carregando...
Citações na Scopus
27
Tipo de produção
article
Data de publicação
2019
Título da Revista
ISSN da Revista
Título do Volume
Editora
HINDAWI LTD
Autores
ZHOU, Qihui
LIGUORI, Tacia Tavares Aquinas
KUHN, Philipp Till
RIJN, Patrick van
HARMSEN, Martin C.
Citação
STEM CELLS INTERNATIONAL, article ID 5387850, 14p, 2019
Resumo
Introduction. Progenitor cells cultured on biomaterials with optimal physical-topographical properties respond with alignment and differentiation. Stromal cells from connective tissue can adversely differentiate to profibrotic myofibroblasts or favorably to smooth muscle cells (SMC). We hypothesized that myogenic differentiation of adipose tissue-derived stromal cells (ASC) depends on gradient directional topographic features. Methods. Polydimethylsiloxane (PDMS) samples with nanometer and micrometer directional topography gradients (wavelength49-3, 425nm) were fabricated. ASC were cultured on patterned PDMS and stimulated with TGF-1 to induce myogenic differentiation. Cellular alignment and adhesion were assessed by immunofluorescence microscopy after 24h. After seven days, myogenic differentiation was examined by immunofluorescence microscopy, gene expression, and immunoblotting. Results. Cell alignment occurred on topographies larger than w=1758nm/a=630nm. The number and total area of focal adhesions per cell were reduced on topographies from w=562nm/a=96nm to w=3919nm/a=1430nm. Focal adhesion alignment was increased on topographies larger than w=731nm/a=146nm. Less myogenic differentiation of ASC occurred on topographies smaller than w=784nm/a=209nm. Conclusion. ASC adherence, alignment, and differentiation are directed by topographical cues. Our evidence highlights a minimal topographic environment required to facilitate the development of aligned and differentiated cell layers from ASC. These data suggest that nanotopography may be a novel tool for inhibiting fibrosis.
Palavras-chave
Referências
- Abagnale G, 2015, BIOMATERIALS, V61, P316, DOI 10.1016/j.biomaterials.2015.05.030
- Aguilar E, 2014, STEM CELLS DEV, V23, P2908, DOI 10.1089/scd.2014.0231
- Aji K, 2016, STEM CELLS INT, DOI 10.1155/2016/1267480
- Liguori TTA, 2018, SCI REP-UK, V8, DOI 10.1038/s41598-018-34747-3
- Au P, 2008, BLOOD, V111, P4551, DOI 10.1182/blood-2007-10-118273
- Bajek A, 2016, ARCH IMMUNOL THER EX, V64, P443, DOI 10.1007/s00005-016-0394-x
- Berginski M. E., 2013, F1000RESEARCH, V2, DOI [10.3410/f1000research.2-68.v1, DOI 10.3410/F1000RESEARCH.2-68.V1]
- Biernacka A, 2011, GROWTH FACTORS, V29, P196, DOI 10.3109/08977194.2011.595714
- Boroujeni SM, 2016, J BIOMED MATER RES A, V104, P1610, DOI 10.1002/jbm.a.35686
- Brohem CA, 2013, INT J COSMETIC SCI, V35, P448, DOI 10.1111/ics.12064
- Charest JL, 2006, BIOMATERIALS, V27, P2487, DOI 10.1016/j.biomaterials.2005.11.022
- Dan P, 2015, J CELL SCI, V128, P2415, DOI 10.1242/jcs.167783
- Dang JM, 2007, ADV MATER, V19, P2775, DOI 10.1002/adma.200602159
- Denu RA, 2016, ACTA HAEMATOL-BASEL, V136, P85, DOI 10.1159/000445096
- Dobaczewski M, 2011, J MOL CELL CARDIOL, V51, P600, DOI 10.1016/j.yjmcc.2010.10.033
- Gautrot JE, 2014, NANO LETT, V14, P3945, DOI 10.1021/nl501248y
- Goffin JM, 2006, J CELL BIOL, V172, P259, DOI 10.1083/jcb.200506179
- Gong ZD, 2011, METHODS MOL BIOL, V698, P279, DOI 10.1007/978-1-60761-999-4_21
- Gong Z, 2009, TISSUE ENG PT A, V15, P319, DOI 10.1089/ten.tea.2008.0161
- Gu WD, 2018, J BIOL CHEM, V293, P8089, DOI 10.1074/jbc.RA118.001739
- Hajmousa G, 2016, STEM CELLS DEV, V25, P1444, DOI 10.1089/scd.2016.0025
- Haniffa MA, 2009, HAEMATOL-HEMATOL J, V94, P258, DOI 10.3324/haematol.13699
- HARRIS L, 2002, THE JOURNAL OF SURGI, V168, P306, DOI 10.1016/J.JSS.2009.08.001
- Hematti P, 2012, CYTOTHERAPY, V14, P516, DOI 10.3109/14653249.2012.677822
- Huang GS, 2015, BIOMATERIALS, V65, P154, DOI 10.1016/j.biomaterials.2015.07.003
- Kulangara K, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0114698
- Kulangara K, 2012, BIOMATERIALS, V33, P4998, DOI 10.1016/j.biomaterials.2012.03.053
- Leask A, 2007, CARDIOVASC RES, V74, P207, DOI 10.1016/j.cardiores.2006.07.012
- Li JG, 2016, COLLOID SURFACE B, V145, P410, DOI 10.1016/j.colsurfb.2016.05.024
- Li Z, 2013, BIOMATERIALS, V34, P7616, DOI 10.1016/j.biomaterials.2013.06.059
- Lin SG, 2017, ACTA BIOMATER, V59, P200, DOI 10.1016/j.actbio.2017.07.012
- Majd H, 2015, BIOMATERIALS, V54, P136, DOI 10.1016/j.biomaterials.2015.03.027
- Mathieu PS, 2012, TISSUE ENG PART B-RE, V18, P436, DOI [10.1089/ten.TEB.2012.0014, 10.1089/ten.teb.2012.0014]
- McCloy RA, 2014, CELL CYCLE, V13, P1400, DOI 10.4161/cc.28401
- McNamara LE, 2010, J TISSUE ENG, V1, DOI 10.4061/2010/120623
- Mushahary D, 2018, CYTOM PART A, V93A, P19, DOI 10.1002/cyto.a.23242
- Newman P, 2016, SCI REP-UK, V6, DOI 10.1038/srep37909
- Oedayrajsingh-Varma MJ, 2006, CYTOTHERAPY, V8, P166, DOI 10.1080/14653240600621125
- Parandakh A, 2018, IN VITRO CELL DEV-AN, V54, P677, DOI 10.1007/s11626-018-0289-8
- PARK I, 2002, JOURNAL OF BIOMATERI, V23, P1579, DOI 10.1163/092050611X587538
- PARK J, 2002, BIOTECHNOLOGY AND BI, V88, P359, DOI 10.1002/BIT.20250
- Parvizi M, 2016, BIOTECHNOL J, V11, P932, DOI 10.1002/biot.201500519
- Piersma B, 2015, FRONT MED, V2, DOI 10.3389/fmed.2015.00059
- Pohlers D, 2009, BBA-MOL BASIS DIS, V1792, P746, DOI 10.1016/j.bbadis.2009.06.004
- Riha GM, 2005, TISSUE ENG, V11, P1535, DOI 10.1089/ten.2005.11.1535
- Steward AJ, 2015, J ANAT, V227, P717, DOI 10.1111/joa.12243
- Teo BKK, 2013, ACS NANO, V7, P4785, DOI 10.1021/nn304966z
- Wang PY, 2012, J MATER SCI-MATER M, V23, P3015, DOI 10.1007/s10856-012-4748-6
- WANG Z, 2002, METHODS, V19, P538, DOI 10.1089/TEN.TEC.2012.0472
- Yao R, 2015, J BIOMECH ENG-T ASME, V137, DOI 10.1115/1.4029255
- Yim EKF, 2007, EXP CELL RES, V313, P1820, DOI 10.1016/j.yexcr.2007.02.031
- Yin Z, 2010, BIOMATERIALS, V31, P2163, DOI 10.1016/j.biomaterials.2009.11.083
- Zhang XQ, 2017, BIOMATERIALS, V145, P9, DOI 10.1016/j.biomaterials.2017.08.028
- Zhou QH, 2017, ACS APPL MATER INTER, V9, P31433, DOI 10.1021/acsami.7b08237
- Zhou QH, 2016, ADV MATER INTERFACES, V3, DOI 10.1002/admi.201600275
- Zhou QH, 2015, SCI REP-UK, V5, DOI 10.1038/srep16240