Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury

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art_BARROS_Evaluation_of_the_effects_of_erythropoietin_and_interleukin6_2019.PDF (476.03 KB)
Citações na Scopus
2
Tipo de produção
article
Data de publicação
2019
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
HOSPITAL CLINICAS, UNIV SAO PAULO
Autores
BARROS, Alderico Girao Campos de
Citação
CLINICS, v.74, article ID e674, 8p, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
OBJECTIVE: To evaluate the effects of interleukin-6 (IL-6) and erythropoietin (EPO) in experimental acute spinal cord injury (SCI) in rats. METHODS: Using standardized equipment, namely, a New York University (NYU) Impactor, a SCI was produced in 50 Wistar rats using a 10-g weight drop from a 12.5-mm height. The rats were divided into the following 5 groups of 10 animals each: ""Group EPO"", treated with erythropoietin only; ""Group EPO + IL-6"", treated with both substances; ""Group IL-6"", receiving IL-6 administration only; ""Group Placebo"", receiving a placebo solution; and ""Group Sham"", submitted to an incomplete procedure (only laminectomy, without SCI). All drugs and the placebo solution were administered intraperitoneally for three weeks. The animals were followed up for 42 days. Functional motor recovery was monitored by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35 and 42. Motor-evoked potential tests were performed on the 42nd day. Histological analysis was performed after euthanasia. RESULTS: The group receiving EPO exhibited superior functional motor results on the BBB scale. IL-6 administration alone was not superior to the placebo treatment, and the IL-6 combination with EPO yielded worse results than did EPO alone. CONCLUSIONS: Using EPO after acute SCI in rats yielded benefits in functional recovery. The combination of EPO and IL-6 showed benefits, but with inferior results compared to those of isolated EPO; moreover, isolated use of IL-6 resulted in no benefit.
Palavras-chave
Interleukin-6, Erythropoietin, Spinal Cord Injuries, Central Nervous System/Injuries, Rats
URI
https://observatorio.fm.usp.br/handle/OPI/33537
Referências
  1. Arishima Y, 2006, SPINE, V31, P2432, DOI 10.1097/01.brs.0000239124.41410.7a
  2. Atkins S, 2007, J PERIPHER NERV SYST, V12, P269, DOI 10.1111/j.1529-8027.2007.00148.x
  3. Bahlmann FH, 2004, BLOOD, V103, P921, DOI 10.1182/blood-2003-04-1284
  4. Basso DM, 1996, EXP NEUROL, V139, P244, DOI 10.1006/exnr.1996.0098
  5. Bethea JR, 2000, PROG BRAIN RES, V128, P33
  6. Bethea JR, 2002, CURR OPIN NEUROL, V15, P355, DOI 10.1097/00019052-200206000-00021
  7. Botchkina GI, 1997, MOL MED, V3, P765, DOI 10.1007/BF03401714
  8. Bracken MB, 1997, JAMA-J AM MED ASSOC, V277, P1597, DOI 10.1001/jama.277.20.1597
  9. Brines M, 2005, NAT REV NEUROSCI, V6, P484, DOI 10.1038/nrn1687
  10. Burnett Mark G, 2004, Neurosurg Focus, V16, pE1, DOI 10.3171/foc.2004.16.5.2
  11. Cayli SR, 2004, EUR SPINE J, V13, P724, DOI 10.1007/s00586-003-0550-y
  12. Celik M, 2002, P NATL ACAD SCI USA, V99, P2258, DOI 10.1073/pnas.042693799
  13. Cristante AF, 2013, SPINAL CORD, V51, P501, DOI 10.1038/sc.2013.18
  14. Cristante AF, 2012, SPINAL CORD, V50, P502, DOI 10.1038/sc.2012.16
  15. Cristante AF, 2009, SPINAL CORD, V47, P733, DOI 10.1038/sc.2009.24
  16. Cristante AF, 2009, SPINAL CORD, V47, P458, DOI 10.1038/sc.2008.155
  17. Cristante AF, 2012, CLINICS, V67, P1219, DOI 10.6061/clinics/2012(10)16
  18. Coutinho PRD, 2016, SPINAL CORD, V54, P439, DOI 10.1038/sc.2015.172
  19. dos Santos GB, 2011, ACTA ORTOP BRAS, V19, P87, DOI 10.1590/S1413-78522011000200005
  20. Ferreira Ricardo, 2005, Acta ortop. bras., V13, P112, DOI 10.1590/S1413-78522005000300002
  21. Fu ES, 2005, CURR OPIN ANESTHESIO, V18, P181, DOI 10.1097/01.aco.0000162838.56344.88
  22. Glick T., 2000, J SPINAL CORD MED S, V23, P61
  23. Gorio A, 2002, P NATL ACAD SCI USA, V99, P9450, DOI 10.1073/pnas.142287899
  24. Grasso G, 2005, NEUROSURGERY, V56, P821, DOI 10.1227/01.NEU.0000156493.00904.7E
  25. Grothe C, 2000, BRAIN RES, V885, P172, DOI 10.1016/S0006-8993(00)02911-5
  26. Hans VHJ, 1999, J CEREBR BLOOD F MET, V19, P184
  27. Hurlbert RJ, 2000, J NEUROSURG, V93, P1, DOI 10.3171/spi.2000.93.1.0001
  28. Inserra MM, 2000, ARCH OTOLARYNGOL, V126, P1112, DOI 10.1001/archotol.126.9.1112
  29. Iyer S, 2010, J NEUROSCI METH, V193, P210, DOI 10.1016/j.jneumeth.2010.08.017
  30. Kaptanoglu E, 2004, NEUROSURG REV, V27, P113, DOI 10.1007/s10143-003-0300-y
  31. Klusman I, 1997, BRAIN RES, V762, P173, DOI 10.1016/S0006-8993(97)00381-8
  32. Kossmann T, 1996, BRAIN RES, V713, P143, DOI 10.1016/0006-8993(95)01501-9
  33. Lee HK, 2009, J NEUROCHEM, V108, P776, DOI 10.1111/j.1471-4159.2008.05826.x
  34. Letaif OB, 2015, CLINICS, V70, P700, DOI 10.6061/clinics/2015(10)08
  35. Mann C, 2008, EXP NEUROL, V211, P34, DOI 10.1016/j.expneurol.2007.12.013
  36. Marcon RM, 2010, SPINAL CORD, V48, P808, DOI 10.1038/sc.2010.37
  37. Marcon RM, 2016, CLINICS, V71, P351, DOI 10.6061/clinics/2016(06)11
  38. Marcon RM, 2010, ACTA ORTOP BRAS, V18, P26, DOI 10.1590/S1413-78522010000100005
  39. Mofidi A, 2011, MINI-REV MED CHEM, V11, P763, DOI 10.2174/138955711796355267
  40. Qian T, 2005, SPINAL CORD, V43, P199, DOI 10.1038/sj.sc.3101681
  41. Schroeter M, 2005, NEUROMOL MED, V7, P183, DOI 10.1385/NMM:7:3:183
  42. Sekiguchi Y, 2003, SPINE, V28, P2577, DOI 10.1097/01.BRS.0000096674.12519.12
  43. Short D, 2001, CURR OPIN NEUROL, V14, P759, DOI 10.1097/00019052-200112000-00013
  44. Sipe KJ, 1996, MOL BRAIN RES, V38, P222, DOI 10.1016/0169-328X(95)00310-O
  45. Squadrito F, 1999, BRIT J PHARMACOL, V127, P482, DOI 10.1038/sj.bjp.0702521
  46. Tansey MG, 2008, FRONT BIOSCI-LANDMRK, V13, P709, DOI 10.2741/2713
  47. Villa P, 2003, J EXP MED, V198, P971, DOI 10.1084/jem.20021067
  48. Yang LQ, 2004, SPINE, V29, P966, DOI 10.1097/00007632-200405010-00004
  49. Zhang Minghuang, 1998, P517

Coleções
Artigos e Materiais de Revistas Científicas - FM/MOT
Artigos e Materiais de Revistas Científicas - HC/IOT
Artigos e Materiais de Revistas Científicas - LIM/24
Artigos e Materiais de Revistas Científicas - LIM/41