High p16(INK4a) immunoexpression is not HPV dependent in oral leukoplakia

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art_TOMO_High_p16INK4a_immunoexpression_is_not_HPV_dependent_in_2020.PDF.pdf (1.02 MB)
Citações na Scopus
10
Tipo de produção
article
Data de publicação
2020
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PERGAMON-ELSEVIER SCIENCE LTD
Autores
TOMO, Saygo
BISS, Stephanye Pinto
CRIVELINI, Marcelo Macedo
OLIVEIRA, Sandra Helena Penha de
BIASOLI, Eder Ricardo
TJIOE, Kellen Cristine
BERNABE, Daniel Galera
MIYAHARA, Glauco Issamu
Citação
ARCHIVES OF ORAL BIOLOGY, v.115, article ID 104738, 7p, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16(INK4a) immunoexpression in the northwest region of the Sao Paulo state, Brazil. Patients and methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 degrees C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16(INK4a) expression was performed. Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression. Conclusion: HPV was not identified in the population studied. The high p16(INK4a) immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions.
Palavras-chave
Oral leukoplakia, Human papillomavirus, Polymerase chain reaction, p16(INK4A)
URI
https://observatorio.fm.usp.br/handle/OPI/36802
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MDR
Artigos e Materiais de Revistas Científicas - LIM/24
Artigos e Materiais de Revistas Científicas - ODS/03