https://observatorio.fm.usp.br/handle/OPI/41418
Title: | Long-term tobacco exposure and immunosenescence: Paradoxical effects on T-cells telomere length and telomerase activity |
Authors: | FERNANDES, Juliana Ruiz; PINTO, Thalyta Nery Carvalho; PIEMONTE, Lucas Lopes; ARRUDA, Lia Barbara; SILVA, Cibele Cristine Berto Marques da; CARVALHO, Celso R. F.; PINTO, Regina Maria Carvalho; DUARTE, Alberto J. S.; BENARD, Gil |
Citation: | MECHANISMS OF AGEING AND DEVELOPMENT, v.197, article ID 111501, 9p, 2021 |
Abstract: | Immunosenescence are alterations on immune system that occurs throughout an individual life. The main characteristic of this process is replicative senescence, evaluated by telomere shortening. Several factors implicate on telomere shortening, such as smoking. In this study, we evaluated the influence of smoking and Chronic Obstructive Pulmonary Disease (COPD) on cytokines, telomere length and telomerase activity. Blood samples were collected from subjects aged over 60 years old: Healthy (never smokers), Smokers (smoking for over 30 years) and COPDs (ex-smokers for >= 15 years). A young group was included as control. PBMCs were cultured for assessment of telomerase activity using RT-PCR, and cytokines secretion flow cytometry. CD4+ and CD8+ purified lymphocytes were used to assess telomere length using FlowFISH. We observed that COPD patients have accelerated telomere shortening. Paradoxically, smokers without lung damage showed preserved telomere length, suggesting that tobacco smoking may affect regulatory mechanisms, such as telomerase. Telomerase activity showed diminished activity in COPDs, while Smokers showed increased activity compared to COPDs and Healthy groups. Extracellular environment reflected this unbalance, indicated by an anti-inflammatory profile in Smokers, while COPDs showed an inflammatory prone profile. Further studies focusing on telomeric maintenance may unveil mechanisms that are associated with cancer under long-term smoking. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - FM/MFT Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/09 Artigos e Materiais de Revistas Científicas - LIM/53 Artigos e Materiais de Revistas Científicas - LIM/54 Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - ODS/03 |
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