Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/48444
Title: | Graphene-based hybrid electrical-electrochemical point-of-care device for serologic COVID-19 diagnosis |
Authors: | MATTIOLI, Isabela A.; CASTRO, Karla R.; MACEDO, Lucyano J. A.; SEDENHO, Graziela C.; OLIVEIRA, Mona N.; TODESCHINI, Iris; VITALE, Phelipe M.; FERREIRA, Suzete Cleusa; MANULI, Erika R.; PEREIRA, Geovana M.; SABINO, Ester C.; CRESPILHO, Frank N. |
Citation: | BIOSENSORS & BIOELECTRONICS, v.199, article ID 113866, 10p, 2022 |
Abstract: | The outbreak of COVID-19 pandemics highlighted the need of sensitive, selective, and easy-to-handle biosensing devices. In the contemporary scenario, point-of-care devices for mass testing and infection mapping within a population have proven themselves as of primordial importance. Here, we introduce a graphene-based Electrical-Electrochemical Vertical Device (EEVD) point-of-care biosensor, strategically engineered for serologic COVID-19 diagnosis. EEVD uses serologic IgG quantifications on SARS-CoV-2 Receptor Binding Domain (RBD) bioconjugate immobilized onto device surface. EEVD combines graphene basal plane with high charge carrier mobility, high conductivity, low intrinsic resistance, and interfacial sensitivity to capacitance alterations. EEVD application was carried out in real human serum samples. Since EEVD is a miniaturized device, it requires just 40 mu L of sample for a point-of-care COVID-19 infections detection. When compared to serologic assays such ELISA and other immunochromatographic methods, EEVD presents some advantages such as time of analyses (15 min), sample preparation, and a LOD of 1.0 pg mL(-1). We glimpse that EEVD meets the principles of robustness and accuracy, desirable analytic parameters for assays destined to pandemics control strategies. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - COVID-19 Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - LIM/31 Artigos e Materiais de Revistas Científicas - LIM/46 Artigos e Materiais de Revistas Científicas - ODS/03 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_MATTIOLI_Graphenebased_hybrid_electricalelectrochemical_pointofcare_device_for_serologic_COVID19_2022.PDF Restricted Access | publishedVersion (English) | 4.43 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.