Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin
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5
Tipo de produção
article
Data de publicação
2014
Editora
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
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Autores
RAYMUNDO, Erica Calcagno
HOCHMAN, Bernardo
NISHIOKA, Michele Akemi
FREITAS, José Octávio Gonçalves de
FERREIRA, Lydia Masako
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Citação
ACTA CIRURGICA BRASILEIRA, v.29, n.4, p.224-230, 2014
Resumo
PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin.
Palavras-chave
Carbon Dioxide, Substance P, Calcitonin Gene-Related Peptide, Neurogenic Inflammation, Skin, Rats