Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/5754
Title: | Effects of subcutaneous carbon dioxide on calcitonin gene related peptide and substance P secretion in rat skin |
Authors: | RAYMUNDO, Erica Calcagno; HOCHMAN, Bernardo; NISHIOKA, Michele Akemi; FREITAS, José Octávio Gonçalves de; MAXIMINO, Jéssica Ruivo; CHADI, Gerson; FERREIRA, Lydia Masako |
Citation: | ACTA CIRURGICA BRASILEIRA, v.29, n.4, p.224-230, 2014 |
Abstract: | PURPOSE: To investigate the subcutaneous injection of carbon dioxide (CO2) on neuropeptides Calcitonin Gene-Related Peptide (CGRP) and Substance P (SP) secretion in rat skin. METHODS: Fifty-six Wistar-EPM rats were distributed in two groups: one for CGRP analysis, the other for SP analysis. Each group was subdivided into four subgroups: control (Cont), control with needle (ContNd), CO2 injection (CO2Inj) and atmospheric air injection (AirInj) - with seven animals each. Sample analyses of partial skin were conducted by Western Blotting (WB). RESULTS: In SP group, there was a decrease in the amount of neuropeptides in subgroups CO2Inj and AirInj. Similarly, in CGRP group, there was a decrease in the amount of pro-CGRP neuropeptides (15 kDa) in subgroups CO2Inj and AirInj; Nevertheless, there was no decrease in the amount of CGRP (5 kDa) in any subgroups. CONCLUSION: Subcutaneous injection of CO2 and atmospheric air decreased the amount of Substance P and pro-Calcitonin Gene-Related Peptide (15 kDa) neuropeptides in rat skin. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MNE Artigos e Materiais de Revistas Científicas - LIM/45 Artigos e Materiais de Revistas Científicas - ODS/13 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_MAXIMINO_Effects_of_subcutaneous_carbon_dioxide_on_calcitonin_gene_2014.PDF | publishedVersion (English) | 801.29 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.