Intestinal Translocation of Clinical Isolates of Vancomycin-Resistant Enterococcus faecalis and ESBL-Producing Escherichia coli in a Rat Model of Bacterial Colonization and Liver Ischemia/Reperfusion Injury

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art_HEIJDEN_Intestinal_Translocation_of_Clinical_Isolates_of_VancomycinResistant_Enterococcus_2014.PDF (201.74 KB)
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16
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article
Data de publicação
2014
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PUBLIC LIBRARY SCIENCE
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Scopus
Web of Science
PubMed
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HEIJDEN, Karin M. van der
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Citação
PLOS ONE, v.9, n.9, article ID e108453, 7p, 2014
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Resumo
The objectives of this study were to develop a rat model of gastrointestinal colonization with vancomycin-resistant Enterococcus faecalis (VRE) and extended-spectrum beta-lactamase (ESBL)-producing E. coli and to evaluate intestinal translocation to blood and tissues after total and partial hepatic ischemia. Methods - We developed a model of rat colonization with VRE and ESBL-E coli. Then we studied four groups of colonized rats: Group I (with hepatic pedicle occlusion causing complete liver ischemia and intestinal stasis); Group II (with partial liver ischemia without intestinal stasis); Group III (surgical manipulation without hepatic ischemia or intestinal stasis); Group IV (anesthetized without surgical manipulation). After sacrifice, portal and systemic blood, large intestine, small intestine, spleen, liver, lungs, and cervical and mesenteric lymph nodes were cultured. Endotoxin concentrations in portal and systemic blood were determined. Results - The best inocula were: VRE: 2.4x10(10) cfu and ESBL-E. coli: 1.12x10(10) cfu. The best results occurred 24 hours after inoculation and antibiotic doses of 750 mu mg/mL of water for vancomycin and 2.1 mg/mL for ceftriaxone. There was a significantly higher proportion of positive cultures for ESBL-E. coli in the lungs in Groups I, II and III when compared with Group IV (67%; 60%; 75% and 13%, respectively; p: 0.04). VRE growth was more frequent in mesenteric lymph nodes for Groups I (67%) and III (38%) than for Groups II (13%) and IV (none) (p:0.002). LPS was significantly higher in systemic blood of Group I (9.761x13.804 EU/mL-p:0.01). No differences for endotoxin occurred in portal blood. Conclusion - e developed a model of rats colonized with resistant bacteria useful to study intestinal translocation. Translocation occurred in surgical procedures with and without hepatic ischemia-reperfusion and probably occurred via the bloodstream. Translocation was probably lymphatic in the ischemia-reperfusion groups. Systemic blood endotoxin levels were higher in the group with complete hepatic ischemia.
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https://observatorio.fm.usp.br/handle/OPI/8988
Referências
  1. Abdala E, 2007, LIVER TRANSPLANT, V13, P488, DOI 10.1002/lt.21085
  2. Alencar SSS, 2001, ACTA CIR BRAS S1, V17, P35
  3. Baumgart DC, 2002, CURR OPIN CLIN NUTR, V5, P685, DOI 10.1097/01.mco.0000038813.16540.3b
  4. Bellot P, 2013, LIVER INT, V33, P31, DOI 10.1111/liv.12021
  5. Brandl K, 2008, NATURE, V455, P804, DOI 10.1038/nature07250
  6. Camilleri M, 2012, AM J PHYSIOL-GASTR L, V303, pG775, DOI 10.1152/ajpgi.00155.2012
  7. CLSI, 2012, M100S22 CLSI
  8. Deitch EA, 2012, SURG-J R COLL SURG E, V10, P350, DOI 10.1016/j.surge.2012.03.003
  9. de Rougemont O, 2009, LIVER TRANSPLANT, V15, P1172, DOI [10.1002/lt.21876, 10.1002/It.21876]
  10. Goyal Ajesh, 2013, Trop Gastroenterol, V34, P136
  11. Grootjans Joep, 2010, World J Gastrointest Surg, V2, P61, DOI 10.4240/wjgs.v2.i3.61
  12. Guarner C, 2006, HEPATOLOGY, V44, P633, DOI 10.1002/hep.21286
  13. Iris NE, 2013, AM J INFECT CONTROL, V41, P261, DOI 10.1016/j.ajic.2012.04.331
  14. Condette CJ, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0102217
  15. Karatzas T, 2014, J SURG RES
  16. Kaufmann ME, 1998, METH MOLEC MED, V15, P33, DOI 10.1385/0-89603-498-4:33
  17. Koh IHJ, 2002, TRANSPLANT P, V34, P1003, DOI 10.1016/S0041-1345(02)02757-4
  18. Liong MT, 2008, NUTR REV, V66, P192, DOI 10.1111/j.1753-4887.2008.00024.x
  19. MacFie J, 1999, GUT, V45, P223
  20. McKinnell JA, 2012, INFECT CONT HOSP EP, V33, P718, DOI 10.1086/666331
  21. Miyazaki S, 2001, J MED MICROBIOL, V50, P695
  22. Murray PR, 2003, MANUAL CLIN MICROBIO
  23. O'Boyle CJ, 1998, GUT, V42, P29
  24. Oplustil CP, 2004, PROCEDIMENTOS BASICO, P544
  25. Papadopoulos Dimitrios, 2013, Arch Trauma Res, V2, P63, DOI 10.5812/atr.12501
  26. Ren ZG, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0075950
  27. Ridley AM, 1998, METH MOLEC MED, V15, P103, DOI 10.1385/0-89603-498-4:103
  28. Swank GM, 1996, WORLD J SURG, V20, P411
  29. TOLEDOPEREYRA LH, 1975, AM J SURG, V129, P513, DOI 10.1016/0002-9610(75)90308-6
  30. Waynforth HB, 1992, EXPT SURG TECHNIQUES, P384
  31. Whitman MS, 1996, ANTIMICROB AGENTS CH, V40, P1526
  32. Zacharioudakis IM, 2014, AM J KIDNEY DIS
  33. Zhai Y, 2013, NAT REV GASTRO HEPAT, V10, P79, DOI 10.1038/nrgastro.2012.225

Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/37
Artigos e Materiais de Revistas Científicas - LIM/54