ISA DIETRICH
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
2 resultados
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- Trophic effects of adipose derived stem cells on Langerhans islets viability - Review(2015) DIETRICH, Isa; CRESCENZI, Alessandra; CHAIB, Elezar; D'ALBUQUERQUE, Luiz Augusto CarneiroLangerhans islets transplantation has been proposed to provide an endogenous source of insulin in Type I diabetes. However, the hypoxic stress and the receptor's immune reaction suffered by the implants cause them to fail in sustaining the insulin production along the time. Experimental studies have shown that adipose derived stem cells (ADSCs) can secrete cytokines that activate free radical scavengers, antioxidants and chaperone heat/shock proteins leading to reduction of apoptosis in damaged tissues. Therefore, using the PubMed database, we reviewed the experimental studies that investigated the trophic effects of ADSCs on Langerhans islets viability, in vitro and in vivo, from 2009 to 2014. We excluded articles that investigated the effects of other types of mesenchymal stem cells on beta-cell survival as well articles that worked in the differentiation of ADSCs into insulin producing cells. The analysis of the experiments revealed that exposure of islets to ADSCs in vitro, even for a short period of time, can enhance islet cell viability and function. In vivo studies also corroborated the trophic effects of ADSCs leading to the improvement of islet function and reduction of the number of the islets required for controlling the receptor's glucose levels. This review can contribute to guide future experiments loolcing for a long term diabetes treatment employing ADSC trophic effects for the enhancement of transplanted Langerhans islet viability and functioning.
conferenceObject ENGRAFTMENT OF HUMAN ADIPOSE DERIVED STEM CELLS DELIVERED IN A HYALURONIC ACID PREPARATION IN MICE(2012) DIETRICH, Isa; COCHET, Olivia; VILLAGEOIS, Phi; RODRIGUES, Consuelo JunqueiraPurpose: To evaluate the implant of human adipose-derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected subcutaneously in athymic mice. Methods: Control implants: HA plus culture media was injected in the subcutaneous of the left subscapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With 8 weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxylin–eosin staining and immunofluorescence using anti-human vimentin and anti-von Willebrand factor antibodies. Results–Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxylin–eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants-A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxylin-eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. Conclusion: The delivery of ADSC in preparations of hyaluronic acid assured cells engraftment at the site of injection.