SHIRLEY VASCONCELOS KOMNINAKIS
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- Re-emergence of mayaro virus and coinfection with chikungunya during an outbreak in the state of Tocantins/Brazil(2022) MARINHO, Robson dos Santos Souza; DURO, Rodrigo Lopes Sanz; CALDEIRA, Debora Bellini; GALINSKAS, Juliana; MOTA, Manlio Tasso Oliveira; HUNTER, James; TELES, Maria da Aparecida Rodrigues; MILAGRES, Flavio Augusto de Padua; DIAZ, Ricardo Sobhie; KAWAKUBO, Fernando Shinji; KOMNINAKIS, Shirley VasconcelosObjective To perform a molecular screening to detect infections by the mayaro virus and possible coinfections with Chikungunya during an outbreak in the state of Tocantins/Brazil in 2017. Results Of a total 102 samples analyzed in this study, 6 cases were identified with simultaneous infection between mayaro and chikungunya viruses (5.88%). In these 6 samples, the mean Cycle threshold (Ct) for CHIKV was 26.87 (SD +/- 10.54) and for MAYV was 29.58 (SD +/- 6.34). The mayaro sequences generated showed 95-100% identity to other Brazilian sequences of this virus and with other MAYV isolates obtained from human and arthropods in different regions of the world. The remaining samples were detected with CHIKV monoinfection (41 cases), DENV monoinfection (50 cases) and coinfection between CHIKV/DENV (5 cases). We did not detect MAYV monoinfections.
- Detection of human feces pecovirus in newly diagnosed HIV patients in Brazil(2022) DURO, Rodrigo Lopes Sanz; MARINHO, Robson dos Santos Souza; SANTANA, Valquiria Lima; MONTI, Elis Muriel Marques; ONIAS, Humberto; MATSUDA, Elaine Monteiro; DIAZ, Ricardo Sobhie; ISIDORO, Karina Rente; DELWART, Eric; LEAL, Elcio; KOMNINAKIS, Shirley VasconcelosCircular single stranded DNA viruses (CRESS DNA) encoding a homologous replication-associated protein (REP) have been identified in most of eukaryotic groups. It is not clear yet the role in human diseases or details of the life cycle of these viruses. Recently, much interest has been raised in the evolutionary history of CRESS DNA owing to the increasing number of new sequences obtained by Next-Generation Sequencing (NGS) in distinct host species. In this study we describe two full-length CRESS DNA genomes obtained of two newly diagnosed HIV patients from Sao Paulo State, Brazil. The initial BLASTx search indicated that both sequences (named SP-FFB/2020 and SP-MJMS/2020) are highly similar (98%) to a previous CRESS DNA sequence detected in human fecal sample from Peru in 2016 and designated as pecovirus (Peruvian stool-associated circo-like virus). This study reported for the first time the Human feces pecovirus in the feces of two newly diagnosed HIV patients in Brazil. Our comparative analysis showed that although pecoviruses in South America share an identical genome structure they diverge and form distinct clades. Thus, we suggest the circulation of different species of pecoviruses in Latin America. Nevertheless, further studies must be done to examine the pathogenicity of this virus.
- Evaluation of the New Multi-HTLV Serological Assay: Improvement for HTLV-2 Detection(2024) FOLGOSI, Victor Angelo; KONMINAKIS, Shirley Vasconcelos; SILVA, Felipe Dias da; LEITE JUNIOR, Pedro Domingos; HAZIOT, Michel Elyas Jung; OLIVEIRA, Augusto C. P.; SMID, Jerusa; ZREIN, Maan; SALVADOR, Florent; CASSEB, JorgeDespite the accuracy of confirmatory tests for the diagnosis of human T cell lymphotropic virus (HTLV), inconclusive or false-negative results still occur when diagnosing human T cell lymphotropic virus type 2 (HTLV-2)-positive patients. The goal of this study was to evaluate the sensitivity and accuracy of a confirmatory immunoassay, the Multi-HTLV assay. A total of 246 plasma samples were tested by real-time polymerase chain reaction (qPCR) and used to calculate the sensitivity and typing accuracy of the Multi-HTLV assay. Of the 246 plasma samples, 127 were positive for human T cell lymphotropic virus type 1 (HTLV-1), 112 were positive for HTLV-2, and 7 were positive for both HTLV-1 and HTLV-2. Thereafter, the nonparametric Mann-Whitney U test was used to calculate the concordance between the qPCR test and Multi-HTLV assay in 12 samples with discrepant and inconclusive qPCR results. The Multi-HTLV assay showed high performance in identifying HTLV-1 and HTLV-2 with sensitivities of 97% [95% confidence interval (CI): 0.92-0.98] and 94% (0.87-0.96), respectively. However, due to typing performance (98% for HTLV-1 and 94% for HTLV-2), it had 95% agreement with positive HTLV-1 qPCR results (95% CI: 90.07-97.81) and 86% (78.04-91.01) of HTLV-2 qPCR results were positive. Moreover, this test was able to recognize 80% of indeterminate samples and all HTLV-2 positive samples that showed false-negative qPCR results. Our findings, derived from a substantial number of HTLV-positive samples, underscore the inherent reliability and feasibility of the Multi-HTLV assay, regardless of the molecular testing facilities. Furthermore, the distinctive multiparametric nature of this assay, combined with its straightforward procedural execution, introduces novel perspectives for analyzing specific serological profiles in each patient, as well as the potential for immunological monitoring of disease progression.
- Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil(2019) CILLI, Audrey; LUCHS, Adriana; LEAL, Elcio; GILL, Danielle; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; LOBATO, Marcia Cristina Alves Brito Sayao; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys daHuman sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols.
- Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil(2019) RIBEIRO, Geovani de Oliveira; MONTEIRO, Fred Julio Costa; REGO, Marlisson Octavio da S.; RIBEIRO, Edcelha Soares D'Athaide; CASTRO, Daniela Funayama de; CASEIRO, Marcos Montani; MARINHO, Robson dos Santos Souza; KOMNINAKIS, Shirley Vasconcelos; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys da; LEAL, ElcioAdvancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
- Detection and Characterization of Enterovirus B73 from a Child in Brazil(2019) RIBEIRO, Geovani de Oliveira; LUCHS, Adriana; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; GILL, Danielle Elise; LOBATO, Marcia Cristina Alves Brito Sayao; BRUSTULIN, Rafael; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; WITKIN, Steven S.; VILLANOVA, Fabiola; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; COSTA, Antonio Charlys da; LEAL, ElcioEnterovirus B73 is a new member of the Enterovirus B species. First detected in the USA, it has been subsequently identified in China, India, Oman, and the Netherlands. In this study, we characterize the first B73 strain (named TO-127) to be detected in South America. TO-127 was obtained from a child with acute gastroenteritis living in a rural area in Northern Brazil. The subject was not infected with any known enteric pathogens such as norovirus, rotavirus, helminths, or enteric bacteria. Analysis of the nearly full-length TO-127 genome (6993 nt) indicated a 74-75% nucleotide similarity with EV-B73 strains from other countries. Evolutionary analysis suggests that B73 is endemic and widespread.
bookPart HIV e subtipos: etiologia(2021) SABINO, Ester Cerdeira; BARRETO, Claudia Cortese; SANABANI, Sabri Saeed; KOMNINAKIS, Shirley Vasconcelos