ANA MARIA RIBEIRO DE CASTRO DUARTE
Projetos de Pesquisa
Unidades Organizacionais
LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Residual malaria of Atlantic Forest systems and the influence of anopheline fauna(2021) FERREIRA, Lucas Mendes; REZENDE, Helder Ricas; BUERY, Julyana Cerqueira; SILVA, Leonardo Santana da; FIGUEIREDO, Thaysa Carolina Cantanhede; FUX, Blima; DUARTE, Ana Maria Ribeiro de Castro; CERUTTI JUNIOR, CrispimIn Brazil, the Amazon region comprises 99.5% of the reported malaria cases. However, another hotspot of the disease is the Atlantic Forest regions, with the sporadic occurrence of autochthonous human cases. In such context, this study sought to investigate the role of anopheline mosquitoes (Diptera: Culicidae) in the residual malaria transmission in Atlantic Forest areas. Two rural areas in the Espirito Santo state were the surveyed sites. Mosquitoes were captured using Shannon trap and CDC light traps and identified into species based on morphological characters. Ecological indexes (Shannon-Wiener diversity, Simpson's dominance, Pielou equability, and Sorensen similarity) were the tools used in the anopheline fauna characterization and comparison along with the two explored areas. The assessment of the sampling adequacy in the studied areas was possible through the generation of a species accumulation curve. A correlation test verified the influence of climatic variables on the anopheline species abundance. A total of 1471 female anopheline mosquitoes were collected from May 2019 to April 2020, representing 13 species. The species richness was higher in Valsugana Velha (hypo-endemic) than in Alto Caparao (non-endemic). There was a significant variation in the species abundance between Valsugana Velha (n = 1438) and Alto Caparao (n = 33). The most abundant species was Anopheles (Kerteszia) cruzii complex Dyar and Knab, 1908 representing 87% of the total anophelines collected. These results suggest that the Plasmodium spp. circulation in Brazilian Atlantic Forest areas occurs mainly due to the high frequency of Anopheles (K.) cruzii complex, considered the principal vector of simian and human malaria in the region.
- Atlantic Forest Malaria: A Review of More than 20 Years of Epidemiological Investigation(2021) BUERY, Julyana Cerqueira; ALENCAR, Filomena Euridice Carvalho de; DUARTE, Ana Maria Ribeiro de Castro; LOSS, Ana Carolina; VICENTE, Creuza Rachel; FERREIRA, Lucas Mendes; FUX, Blima; MEDEIROS, Marcia Melo; CRAVO, Pedro; AREZ, Ana Paula; CERUTTI JUNIOR, CrispimIn the south and southeast regions of Brazil, cases of malaria occur outside the endemic Amazon region near the Atlantic Forest in some coastal states, where Plasmodium vivax is the recognized parasite. Characteristics of cases and vectors, especially Anopheles (Kerteszia) cruzii, raise the hypothesis of a zoonosis with simians as reservoirs. The present review aims to report on investigations of the disease over a 23-year period. Two main sources have provided epidemiological data: the behavior of Anopheles vectors and the genetic and immunological aspects of Plasmodium spp. obtained from humans, Alouatta simians, and Anopheles spp. mosquitoes. Anopheles (K.) cruzii is the most captured species in the forest canopy and is the recognized vector. The similarity between P. vivax and Plasmodium simium and that between Plasmodium malariae and Plasmodium brasilianum shared between simian and human hosts and the involvement of the same vector in the transmission to both hosts suggest interspecies transfer of the parasites. Finally, recent evidence points to the presence of Plasmodium falciparum in a silent cycle, detected only by molecular methods in asymptomatic individuals and An. (K.) cruzii. In the context of malaria elimination, it is paramount to assemble data about transmission in such non-endemic low-incidence areas.
- Plasmodium simium: Population Genomics Reveals the Origin of a Reverse Zoonosis(2021) OLIVEIRA, Thais C. de; RODRIGUES, Priscila T.; EARLY, Angela M.; DUARTE, Ana Maria R. C.; BUERY, Julyana C.; BUENO, Marina G.; CATAO-DIAS, Jose L.; CERUTTI, Crispim; RONA, Luisa D. P.; NEAFSEY, Daniel E.; FERREIRA, Marcelo U.Background. The population history of Plasmodium simium, which causes malaria in sylvatic Neotropical monkeys and humans along the Atlantic Coast of Brazil, remains disputed. Genetically diverse P vivax populations from various sources, including the lineages that founded the species P simium, are thought to have arrived in the Americas in separate migratory waves. Methods. We use population genomic approaches to investigate the origin and evolution of P simium. Results. We find a minimal genome-level differentiation between P simium and present-day New World P vivax isolates, consistent with their common geographic origin and subsequent divergence on this continent. The meagre genetic diversity in P simium samples from humans and monkeys implies a recent transfer from humans to non-human primates - a unique example of malaria as a reverse zoonosis of public health significance. Likely genomic signatures of P simium adaptation to new hosts include the deletion of > 40% of a key erythrocyte invasion ligand, PvRBP2a, which may have favored more efficient simian host cell infection. Conclusions. New World P vivax lineages that switched from humans to platyrrhine monkeys founded the P simium population that infects nonhuman primates and feeds sustained human malaria transmission in the outskirts of major cities.
- Complexity of malaria transmission dynamics in the Brazilian Atlantic Forest(2021) DUARTE, Ana Maria Ribeiro de Castro; FERNANDES, Licia Natal; SILVA, Fabiana Santos; SICCHI, Igor Lucoves; MUCCI, Luis Filipe; CURADO, Izilda; FERNANDES, Aristides; MEDEIROS-SOUSA, Antonio Ralph; CERETTI-JUNIOR, Walter; MARRELLI, Mauro Toledo; EVANGELISTA, Eduardo; TEIXEIRA, Renildo; SUMMA, Juliana Laurito; NARDI, Marcello Schiavo; GARNICA, Margoth Ramos; LOSS, Ana Carolina; BUERY, Julyana Cerqueira; CERUTT, Crispim; PACHECO, M. Andreina; ESCALANTE, Ananias A.; SALLUM, Maria Anice Mureb; LAPORTA, Gabriel ZorelloPlasmodium malariae and Plasmodium vivax are protozoan parasites that can cause malaria in humans. They are genetically indistinguishable from, respectively, Plasmodium brasilianum and Plasmodium simium, i.e. parasites infecting New World non-human primates in South America. In the tropical rainforests of the Brazilian Atlantic coast, it has long been hypothesized that P. brasilianum and P. simium in platyrrhine primates originated from P. malariae and P. vivax in humans. A recent hypothesis proposed the inclusion of Plasmodium falciparum into the transmission dynamics between humans and non-human primates in the Brazilian Atlantic tropical rainforest. Herein, we assess the occurrence of human malaria in simians and sylvatic anophelines using field-collected samples in the Capivari-Monos Environmental Protection Area from 2015 to 2017. We first tested simian blood and anopheline samples. Two simian (Aloutta) blood samples (18%, n = 11) showed Plasmodium cytb DNA sequences, one for P. vivax and another for P. malariae. From a total of 9,416 anopheline females, we found 17 pools positive for Plasmodium species with a 18S qPCR assay. Only three showed P. cytb DNA sequence, one for P. vivax and the others for rodent malaria species (similar to Plasmodium chabaudi and Plasmodium berghei). Based on these results, we tested 25 rodent liver samples for the presence of Plasmodium and obtained P. falciparum cytb DNA sequence in a rodent (Oligoryzomys sp.) liver. The findings of this study indicate complex malaria transmission dynamics composed by parallel spillover-spillback of human malaria parasites, i.e. P. malariae, P. vivax, and P. falciparum, in the Brazilian Atlantic forest.
- Ongoing host-shift speciation in Plasmodium simium(2021) OLIVEIRA, Thais C. de; RODRIGUES, Priscila T.; DUARTE, Ana Maria R. C.; RONA, Luisa D. P.; FERREIRA, Marcelo U.Plasmodium simium, a malaria parasite that infects platyrrhine monkeys and humans in the New World, is nearly identical to Plasmodium vivax. Recent genomic comparative analyses of these sister species have identified elevated divergence in a gene that may underlie P. simium adaptation to non-human primates during its gradual speciation process.