FERNANDA YAMAMOTO RICARDO DA SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação, Hospital das Clínicas, Faculdade de Medicina
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject FEMALE RATS PRESENT HIGHER LUNG INFLAMMATION AFTER BRAIN DEATH FOLLOWED BY EX VIVO PERFUSION(2021) RICARDO-DA-SILVA, Fernanda Yamamoto; ARMSTRONG- JR., Roberto; OTTENS, Petra; ZANDEN, Judith van; VIDAL-DOS-SANTOS, Marina; MOREIRA, Luiz Felipe Pinho; ERASMUS, Michiel; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana CristinaconferenceObject ESTRADIOL TREATMENT MODULATES ESTRADIOL RECEPTORS EXPRESSION AND REDUCES RENAL INJURY AFTER BRAIN DEATH IN FEMALE RATS(2021) CORREIA, Cristiano; ARMSTRONG- JR., Roberto; RICARDO-DA-SILVA, Fernanda Yamamoto; VIDAL-DOS-SANTOS, Marina; ANUNCIACAO, Lucas Ferreira Da; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana Cristina- The influence of female sex hormones on lung inflammation after brain death - an experimental study(2020) ABIB, Ana Luisa de Oliveira Bonnano; CORREIA, Cristiano de Jesus; ARMSTRONG- JR., Roberto; RICARDO-DA-SILVA, Fernanda Yamamoto; FERREIRA, Sueli Gomes; VIDAL-DOS-SANTOS, Marina; MOREIRA, Luiz Felipe Pinho; RIFFO-VASQUEZ, Yanira; BREITHAUPT-FALOPPA, Ana CristinaOrgan donor's age negatively influences graft survival of organs, increasing risk of complications. Aging occurs in both men and women; however, the menopause marks a decrease in sex hormones and a sudden increase in the process of vascular aging. We investigated sex hormones' influence on the lung inflammatory process induced by BD in female rats. Wistar rats were grouped as: female rats from high estradiol to heat period (non-OVx) and ovariectomized (OVx) female rats. Ovariectomy was carried out 10 days before BD. BD was induced using intracranial balloon rapid inflation. Serum hormones and inflammatory mediators were quantified, leukocytes and platelets counted and lung samples were collected for RT-PCR, immunohistochemical, and histological analysis. Female sex hormones and corticosterone were reduced 6 h after BD in non-OVx group. The infiltration of leukocytes in female non-OVx lungs was higher compared to OVx. G-CSF, VEGF, and CINC-1 were found increased in non-OVx group serum in comparison to OVx. Lung mediators were increased in non-OVx rats compared to controls. The acute reduction of sex hormones induced by BD appears to have a worse effect on lung inflammation than a reduction that has happened over a prolonged period of time, allowing a physiological adaptation prior to BD.
conferenceObject Kidney Release of Inflammatory Mediators Is Modulated by 17BETA-Estradiol Associated With Methylprednisolone After Brain Death in Female Rats(2022) SANTOS, Marina Vidal dos; ANUNCIACAO, Lucas Ferreira da; ARMSTRONG JR., Roberto; SILVA, Fernanda Yamamoto Ricardo da; RAMOS, Mayara Munhoz de Assis; CORREIA, Cristiano de Jesus; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri; FALOPPA, Ana Cristina Breithaupt- Treatment with 17 beta-estradiol protects donor heart against brain death effects in female rat(2020) ARMSTRONG-JR, Roberto; RICARDO-DA-SILVA, Fernanda Yamamoto; CORREIA, Cristiano Jesus; VIDAL-DOS-SANTOS, Marina; ANUNCIACAO, Lucas Ferreira da; SILVA, Raphael Santos Coutinho e; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Hendrik Gerrit Derk; BREITHAUPT-FALOPPA, Ana CristinaThe viability of donor organs is reduced by hemodynamic and immunologic alterations caused by brain death (BD). Female rats show higher heart inflammation associated with the reduction in female sex hormones after BD. This study investigated the effect of 17 beta-estradiol (E2) on BD-induced cardiac damage in female rats. Groups of female Wistar rats were assigned: Sham-operation (Sham), brain death (BD), treatment with E2 (50 mu g/ml, 2 ml/h) 3 h after BD (E2-T3), or immediately after BD confirmation (E2-T0). White blood cell (WBC) count was analyzed; cytokines and troponin-I were quantified. Heart histopathological changes and expression of endothelial nitric oxide synthase, endothelin-1, intercellular adhesion molecule-1, BCL-2, and caspase-3 were evaluated. Cardiac function was continuously assessed for 6 h by left ventricular pressure-volume loop analysis. E2 decreased the BD-induced median serum concentration of troponin-I (BD:864.2 vs. E2-T0:401.4;P = 0.009), increased BCL-2 (BD:0.086 vs. E2-T0:0.158; P = 0.0278) and eNOS median expression in the cardiac tissue (BD:0.001 vs. E2-T0:0.03 and E2-T3:0.0175; P < 0.0001), and decreased caspase-3 (BD:0.025 vs. E2-T0:0.006 and E2-T3:0.019; P = 0.006), WBC counts, leukocyte infiltration, and hemorrhage. 17 beta-estradiol treatment was effective in reducing cardiac tissue damage in brain-dead female rats owing to its ability to reduce leukocyte infiltration and prevent cardiomyocyte apoptosis.
conferenceObject Sex Differences in Acute Kidney Injury Following BD - An Isolated Rat Organ Perfusion Model(2022) ARMSTRONG JUNIOR, Roberto; RICARDO-DA-SILVA, Fernanda F. Yamamoto; VIDAL-DOS-SANTOS, Marina M.; OTTENS, Petra; CORREIA, Cristiano C. Jesus; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri H.; BREITHAUPTO-FALOPPA, Ana Cristina A. C.conferenceObject 17beta-Estradiol and Methylprednisolone Associated Treatment Modulates Early and Late Cytokine Release in Lungs From Female Rats Submitted to Brain Death(2022) SANTOS, Marina Vidal dos; ANUNCIACAO, Lucas Ferreira da; ARMSTRONG JR., Roberto; SILVA, Fernanda Yamamoto Ricardo da; RAMOS, Mayara Munhoz de Assis; CORREIA, Cristiano de Jesus; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri; FALOPPA, Ana Cristina BreithauptconferenceObject EVALUATION OF SEX DIFFERENCES IN ACUTE KIDNEY INJURY AFTER BD USING AN ISOLATED PERFUSED RAT KIDNEY MODEL(2021) ARMSTRONG- JR., Roberto; RICARDO-DA-SILVA, Fernanda Yamamoto; VIDAL-DOS-SANTOS, Marina; OTTENS, Petra; CORREIA, Cristiano; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana Cristina- Sex differences in the coagulation process and microvascular perfusion induced by brain death in rats(2020) CORREIA, Cristiano de Jesus; SILVA, Fernanda Yamamoto Ricardo da; ARMSTRONG, Roberto Junior; SANTOS, Marina Vidal dos; ANUNCIACAO, Lucas Ferreira da; SOBRAL, Marcelo Luiz Peixoto; SILVA, Raphael dos Santos Coutinho e; LEUVENINK, Hendrik Gerrit Derk; BREITHAUPT-FALOPPA, Ana Cristina; MOREIRA, Luiz Felipe PinhoBrain death (BD) leads to a systemic inflammation associated with the activation of coagulation, which could be related to decreased microcirculatory perfusion. Evidence shows that females exhibit higher platelet aggregability than males. Thus, we investigated sex differences in platelets, coagulation and microcirculatory compromise after BD. BD was induced in male and female (proestrus) Wistar rats. After 3 h, we evaluated: (i) intravital microscopy to evaluate mesenteric perfusion and leucocyte infiltration; (ii) platelet aggregation assay; (iii) rotational thromboelastometry; and (iv) SerumNOx-. Female rats maintained the mesenteric perfusion, whereas male reduced percentage of perfused vessels. Male BD presented higher platelet aggregation than the controls. In contrast, female BD had lower platelet aggregation than the control. Thromboelastometry indicated a reduction in clot firmness with increased clotting time in the female group compared with the male group. SerumNOx-level in female BD was higher than that in the male BD and female control. There is sex dimorphism in platelet function and clotting process, which are altered in different ways by BD. Thus, it is possible to connect the reduction in microcirculatory perfusion in males to intravascular microthrombi formation and the maintenance of perfusion in females to a higher inflammatory response and NO synthesis.
conferenceObject ESTRADIOL TREATMENT REDUCES LUNG INFLAMMATION INDUCED BY BRAIN DEATH IN FEMALE RATS(2017) SILVA, Fernanda Yamamoto Ricardo Da; AMSTRONG JR., Roberto; SANTOS, Marina Vidal Dos; FERREIRA, Sueli Gomes; SANNOMIYA, Paulina; MOREIRA, Luiz Felipe; BREITHAUPT-FALOPPA, Ana Cristina