DENNIS MINORU FUJITA

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LIM/49 - Laboratório de Protozoologia, Hospital das Clínicas, Faculdade de Medicina

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  • article 0 Citação(ões) na Scopus
    Silent spread of DENV-3 in Brazil: autochthonous outbreak in São Paulo after 15 years
    (2024) FUJITA, Dennis M.; SALVADOR, Felipe S.; NALI, Luiz H. da Silva; ANDRADE JUNIOR, Heitor F. de
    The high incidence of dengue in Brazil and re-emergence of DENV-3 in SAo Paulo after a 15-year absence, coupled with the potential risk for DENV-4, may be the results from changing climate patterns favouring Aedes mosquito proliferation, allied by the resuming of domestic and international travels.
  • article 0 Citação(ões) na Scopus
    Epidemiological profile and genetic resistance of Neisseria gonorrhoeae infection in women in a poor region of Sao Paulo, Brazil
    (2024) TAVARES, Gabriela Martinez Moura; ALVERGA, Hannar Angelica de Melo; FELIX, Estela Moraes; DICHMAN, Giovana de Oliveira; PINHEIRO, Gabriel Monteiro; JULIANO, Yara; PARUCI, Priscila; FUJITA, Dennis Minoru; NEVES, Lucas Melo; SHIO, Marina Tiemi; NALI, Luiz Henrique da Silva
    Background and aim: Gonorrhea is a bacterial infection in the urogenital tract, transmitted by sexual or perinatal contact, caused by Neisseria gonorrhoeae, a gram-negative diplococcus. The present study evaluates the frequency of N. gonorrhoeae in women treated at Hospital Wladimir Arruda in poor area of Sa similar to o Paulo and also verifies the presence of genetic resistance against three antimicrobials of different classes: Tetracycline, Azithromycin and Ciprofloxacin. Methods: This is an observational and descriptive study with a quantitative approach. Samples were collected at Hospital Escola Wladimir Arruda. The volunteers are women from 16 to 65 years of age. Sociodemographic, gynecological, sexual and health data are collected through a questionnaire, their symptoms/clinical manifestation were requested by the medical records, and then the participant is referred for collection of samples of cervical vaginal smear. The samples were screened for N. gonorrhoeae (dcmH gene) and tested for resistance genes to Tetracycline, Azithromycin and Ciprofloxacin through PCR. Results: In the total of 127 samples analyzed by Real-Time PCR, 23 were positive and correspond to a general prevalence of a gonococcal infection in the studied population of 17% (CI:95%), and the participants were married (43.4%), had active sexual life (56.5%) and did not use any type of condom during sexual intercourse (52.1%). The resistance to the tetM ribosomal gene was found in 14 samples, prevalence of 60% (CI= 95%). Conclusions: We have described a concerning frequency of N. gonorrhoeae infection in females attended in an outcare patient. Also, most of the strains detected presented resistance to one or more antimicrobials.