JULIO YOSHIO TAKADA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
3 resultados
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- Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after Caloric Restriction and Resveratrol Administration(2018) ROGGERIO, Alessandra; STRUNZ, Celia M. Cassaro; PACANARO, Ana Paula; LEAL, Dalila Pinheiro; TAKADA, Julio Y.; AVAKIAN, Solange D.; MANSUR, Antonio de PaduaSirtuin-1 (Sirt-1) and an endogenous secretory receptor for an advanced glycation end product (esRAGE) are associated with vascular protection. The purpose of this study was to examine the effects of resveratrol (RSV) and caloric restriction (CR) on gene expression of Sirt-1 and esRAGE on serum levels of Sirt1 and esRAGE in healthy and slightly overweight subjects. The study included 48 healthy subjects randomized to 30 days of RSV (500 mg/day) or CR (1000 cal/day). Waist circumference (p = 0.011), TC (p = 0.007), HDL (p = 0.031), non-HDL (p = 0.025), ApoA1 (p = 0.011), and ApoB (p = 0.037) decreased in the CR group. However, TC (p = 0.030), non-HDL (p = 0.010), ApoB (p = 0.034), and HOMA-IR (p = 0.038) increased in the RSV group. RSV and CR increased serum levels of Sirt-1, respectively, from 1.06 +/- 0.71 ng/mL to 5.75 +/- 2.98 ng/mL (p < 0.0001) and from 1.65 +/- 1.81 ng/mL to 5.80 +/- 2.23 ng/mL (p < 0.0001). esRAGE serum levels were similar in RSV (p = NS) and CR (p = NS) groups. Significant positive correlation was observed between gene expression changes of Sirt-1 and esRAGE in RSV (r = 0.86; p < 0.0001) and in CR (r = 0.71; p < 0.0001) groups, but not for the changes in serum concentrations. CR promoted increases in the gene expression of esRAGE (post/pre). Future long-term studies are needed to evaluate the impact of these outcomes on vascular health.
- Influence of the treatment of periodontal disease in serum concentration of sirtuin 1 and mannose-binding lectin(2020) CARIBE, Perola Michelle Vasconcelos; VILLAR, Cristina Cunha; ROMITO, Giuseppe Alexandre; PACANARO, Ana Paula; STRUNZ, Celia Maria Cassaro; TAKADA, Julio Yoshio; CESAR, Luiz Antonio Machado; MANSUR, Antonio de PaduaBackground Increased levels of periodontal pathogens disrupt the homeostasis between the host and its microbiota and increase susceptibility to periodontal diseases. Periodontitis increases the serum concentration of mannose-binding lectin (MBL), which exacerbates local inflammatory processes. In animal studies, sirtuin 1 (SIRT1) was associated with protection against inflammation. This study analyzed the influence of non-surgical periodontal treatment on serum levels of MBL and SIRT1. Methods Forty patients with periodontitis and 38 periodontally healthy individuals (aged 45 to 79 years) were included. Periodontitis patients received scaling and root planing using machine driven and hand instruments. Clinical parameters, inflammatory biomarkers, MBL, and SIRT1 levels were measured at baseline and at post-treatment. Results For all patients, an inverse correlation was observed between serum concentrations of MBL and SIRT1 (r = -0.30; P = 0.006). Periodontal treatment reduced serum concentrations of MBL (1,099.35 +/- 916.59 to 861.42 +/- 724.82 ng/mL; P < 0.001) and C-reactive protein (6.05 +/- 8.99 to 2.49 +/- 2.89 mg/L; P = 0.009). By contrast, SIRT1 serum levels increased (1.06 +/- 1.03 to 1.66 +/- 1.64 ng/mL; P < 0.001) following periodontal treatment. Conclusions Periodontal treatment was associated with decreased serum concentrations of MBL and CRP and increased serum levels of SIRT1. Prospective studies are needed to assess the impact of these biomarkers on pathophysiology of periodontitis.
- Prospective, case-controlled study evaluating serum concentration of sirtuin-1 and mannose-binding lectin in patients with and without periodontal and coronary artery disease(2020) CARIBE, Perola Michelle Vasconcelos; VILTAR, Cristina Cunha; ROMITO, Guiseppe Alexandre; TAKADA, Julio Yoshio; PACANARO, Ana Paula; STRUNZ, Celia Maria Cassaro; CESAR, Luiz Antonio Machado; MANSUR, Antonio de PaduaBackground: Atherosclerosis and periodontal disease (PD) are inflammatory diseases that have been shown in studies to have a direct association. Mannose-binding lectin (MBL) is an immune system protein that binds to periodontal pathogens favoring phagocytosis. Conversely, increased serum sirtuin-1 (SIRT1) concentration reduces the inflammatory process. Methods: This was a prospective, case-controlled study that analyzed serum concentration of biomarkers in patients with or without coronary artery disease (CAD) and PD. A total of 78 patients were evaluated: 20 healthy individuals, 18 patients with CAD, 20 patients with PD, and 20 patients with both PD and CAD. Clinical and laboratory characteristics were analyzed before and after nonsurgical treatment of PD and also at two equivalent times in patients without PD. Serum MBL and SIRT1 concentration were analyzed by enzyme-linked immunosorbent assay. Results: A negative correlation was observed between changes in serum concentration of MBL and SIRT1 (r = -0.30; p = 0.006). Comparison between pre- and post-treatment of PD showed a reduction in MBL levels (886.27 +/- 906.72 versus 689.94 +/- 808.36; p = 0.002) and an increase in SIRT1 values (0.80 +/- 1.01 versus 1.49 +/- 1.55; p = 0.005) in patients with PD and without CAD. The same result was observed in patients with PD and CAD for MBL and SIRT1, respectively, of 1312.43 +/- 898.21 versus 1032.90 +/- 602.52 (p = 0.010) and 1.32 +/- 1.0 versus 1.82 +/- 1.75 (p = 0.044). Conclusion: PD treatment reduced MBL serum concentration and increased SIRT1 serum concentration in patients with and without CAD.