GILBERTO DE CASTRO JUNIOR
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
17 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 17
- The wolf in sheep's clothing: Microtomographic aspects of clinically incipient radiation-related caries(2016) MORAIS-FARIA, Karina; NEVES-SILVA, Rodrigo; LOPES, Marcio-Ajudarte; RIBEIRO, Ana-Carolina-Prado; CASTRO JR., Gilberto de; CONCEICAO-VASCONCELOS, Karina-Gondim-Moutinho da; BRANDAO, Thais-Bianca; SANTOS-SILVA, Alan-RogerBackground: Radiation-related caries (RRC) can cause rapid progression, with a high potential for dental destruction affecting mainly cervical and incisal areas. Unlike the injuries that occur in the conventional caries, incipient RRC present in unusual surfaces have difficult diagnosis and classification stages of cavitation. Material and Methods: Evaluate the radiographic patterns of demineralization of RRC by using micro-CT. Ten teeth with incipient RRC and 10 teeth with incipient conventional caries (control group) matched by anatomic teeth group and caries affected surfaces were evaluated by X-ray microtomography (micro-CT) Skyscan 1174V2 (50Kv, 1.3 megapixel, Kontich, Belgium). Teeth were placed in a standard position for micro-CT (coronal, transaxial and sagittal sections) during images acquisition. Lesions were classified according to the depth of invasion and relationship with enamel, dentin and pulp. Results: RRC samples presented deeper lesions with higher involvement of enamel and dentin. Control group presented focal and superficial lesions with lower involvement of enamel and dentin. Conclusions: Incipient RRC present aggressive microtomographic patterns of demineralization when compared to conventional caries, as indicated by deep lesions, regardless of its clinically incipient aspects.
- Systemic therapies for salivary gland carcinomas: an overview of published clinical trials(2024) SILVA, Luan Cesar; PEREZ-DE-OLIVEIRA, Maria Eduarda; PEDROSO, Caique Mariano; LEITE, Amanda Almeida; SANTOS-SILVA, Alan Roger; LOPES, Marcio Ajudarte; CASTRO JUNIOR, Gilberto de; MARTINS, Manoela Domingues; WAGNER, Vivian Petersen; KOWALSKI, Luiz Paulo; SQUARIZE, Cristiane Helena; CASTILHO, Rogerio Moraes; VARGAS, Pablo AgustinBackground: There is no consensus about effective systemic therapy for salivary gland carcinomas (sgcs). Our aim was summarized the clinical trials assessing the systemic therapies (ST) on sgcs. Material and Methods: Electronic searches were carried out through MEDLINE/pubmed, EMBASE, Scopus, Web of Science, and the Cochrane Library databases, and gray literature. Results: Seventeen different drugs were evaluated, and the most frequent histological subtype was adenoid cystic carcinoma (n=195, 45.5%). Stable disease, observed in 11 ST, achieved the highest rate in adenoid cystic carcinoma treated with sunitinib. The highest complete (11.1%) and partial response (30.5%) rates were seen in androgen receptor -positive tumors treated with leuprorelin acetate. Conclusions: Despite all the advances in this field, there is yet no effective evidence -based regimen of ST, with all the clinical trials identified showing low rates of complete and partial responses. Further, translational studies are urgently required to characterize molecular targets and effective ST.
- Patient's perceptions of oral and oropharyngeal cancer diagnosis disclosure: communication aspects based on SPIKES protocol(2023) ALVES, Carolina G. B.; RIBEIRO, Ana Carolina Prado; BRANDAO, Thais Bianca; TONAKI, Juliana O.; PEDROSO, Caique Mariano; RIVERA, Cesar; EPSTEIN, Joel B.; MIGLIORATI, Cesar Augusto; KOWALSKI, Luiz Paulo; MAK, Milena Perez; CASTRO, Gilberto; LOPES, Marcio Ajudarte; SANTOS-SILVA, Alan RogerObjective. Breaking bad medical news is a complex task of clinical practice. The manner in which this is done has a significant impact on patients. This study aimed to assess patient's perceptions regarding oral and oropharyngeal cancer diagnosis disclosure according to the ""SPIKES"" protocol. Study Design. This cross-sectional study used a questionnaire with 21 SPIKES-based items. The questionnaire was administered to 100 patients with recently diagnosed oral and oropharyngeal squamous cell carcinoma who evaluated each item according to their preference and experience. Results. Nineteen items showed a significant difference between patient's preference and recalled experience. Eighteen of these items showed lower experience scores primarily related to the amount of information desired by patients, presence of a compan-ion, time to express feelings, and summary of information. Most patients preferred receiving as much information as possible about the diagnosis. However, only 35% reported that they had obtained sufficient information. Patients who were aware of can-cer diagnostic suspicion had better communication experiences. Conclusions. Protocols may be useful to guide health professionals to support patient-centered strategies to disclose oral cancer diagnoses. (Oral Surg Oral Med Oral Pathol Oral Radiol 2023;135:518-529)
- Impact of radiation on tooth loss in patients with head and neck cancer: a retrospective dosimetric-based study(2021) GOMES-SILVA, Wagner; MORAIS-FARIA, Karina; RIVERA, Cesar; NAJAS, Gabriel Faria; MARTA, Gustavo Nader; VASCONCELOS, Karina Gondim Moutinho da Conceicao; CARVALHO, Heloisa de Andrade; CASTRO JR., Gilberto de; BRANDAO, Thais Bianca; EPSTEIN, Joel B.; SANTOS-SILVA, Alan RogerObjective. To characterize the dental adverse events after head and neck radiation therapy (HNRT) and to investigate the impact of regional radiation dose upon tooth loss outcomes. Study Design. A retrospective dosimetric-based analysis was conducted to assess dental events affecting post-HNRT extracted teeth and the impact of 3 different radiation doses ( 30 Gy, 30-60 Gy, and .001). The odds ratio for post-HNRT tooth extraction risk was approximately 3-fold higher for teeth exposed to 60 Gy (confidence interval, 1.56-5.35; P < .001), followed by an increased risk of delayed healing and osteoradionecrosis (ORN) in sites receiving doses above 50 Gy. Conclusions. Radiation caries was the major cause of dental extractions after HNRT, and the dosimetric analysis suggested that a high dose of radiation may negatively impact the dentition of survivors of head and neck cancer, increasing the risk of tooth loss and ORN. (Oral Surg Oral Med Oral Pathol Oral Radiol 2021;132:409-417)
- Cephaeline is an inductor of histone H3 acetylation and inhibitor of mucoepidermoid carcinoma cancer stem cells(2022) SILVA, Luan Cesar; BORGATO, Gabriell Bonifacio; WAGNER, Vivian Petersen; MARTINS, Manoela Domingues; ROCHA, Guilherme Zweig; LOPES, Marcio Ajudarte; SANTOS-SILVA, Alan Roger; CASTRO JUNIOR, Gilberto de; KOWALSKI, Luiz Paulo; NOR, Jacques E.; SQUARIZE, Cristiane H.; CASTILHO, Rogerio Moraes; VARGAS, Pablo AgustinAim To evaluate the potential use of Cephaeline as a therapeutic strategy to manage mucoepidermoid carcinomas (MEC) of the salivary glands. Material and Methods UM-HMC-1, UM-HMC-2, and UM-HMC-3A MEC cell lines were used to establish the effects of Cephaeline over tumor viability determined by MTT assay. In vitro wound healing scratch assays were performed to address cellular migration while immunofluorescence staining for histone H3 lysine 9 (H3k9ac) was used to identify the acetylation status of tumor cells upon Cephaeline administration. The presence of cancer stem cells was evaluated by the identification of ALDH enzymatic activity by flow cytometry and through functional assays using in vitro tumorsphere formation. Results A single administration of Cephaeline resulted in reduced viability of MEC cells along with the halt on tumor growth and cellular migration potential. Administration of Cephaeline resulted in chromatin histone acetylation as judged by the increased levels of H3K9ac and disruption of tumorspheres formation. Interestingly, ALDH levels were increased in UM-HMC-1 and UM-HMC-3A cell lines, while UM-HMC-2 showed a reduced enzymatic activity. Conclusion Cephaeline has shown anti-cancer properties in all MEC cell lines tested by regulating tumor cells' viability, migration, proliferation, and disrupting the ability of cancer cells to generate tumorspheres.
- Oral leukoplakia and erythroplakia in young patients: a systematic review(2021) ROZA, Ana Luiza Oliveira Correa; KOWALSKI, Luiz Paulo; JR, William Nassib William; JR, Gilberto de Castro; CHAVES, Aline Lauda Freitas; ARAUJO, Anna Luiza Damaceno; RIBEIRO, Ana Carolina Prado; BRANDAO, Thais Bianca; LOPES, Marcio Ajudarte; VARGAS, Pablo Agustin; SANTOS-SILVA, Alan RogerObjective. The aim of this study was to estimate the frequency of oral leukoplakia and oral erythroplakia in young patients. Study Design. The systematic review was based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines and performed in the following electronic databases: PubMed, Scopus, and Embase. The studies included were cross-sectional, cohort, and diagnostic, which provided with clinical and microscopic data of patients younger than 40 years. The Critical Appraisal Checklist for Prevalence Studies from the Joanna Briggs Institute and the Quality Assessment Tool for Diagnostic Accuracy Studies were used to assess the risk of bias. Results. Five studies met eligibility criteria and were included. The total number of patients from the studies was 1246, of which 115 were young patients (9.2%) with oral leukoplakia as the only oral potentially malignant disorder reported. Oral epithelial dysplasia was identified in 40 cases (34.7%), of which 8 (6.9%) presented malignant transformation. Conclusions. The frequency of oral leukoplakia is low in young patients. Observational studies are necessary for understanding oral leukoplakia and other oral potentially malignant disorders in younger patients.
- Head and neck radiotherapy does not increase gelatinase (metalloproteinase-2 and-9) expression or activity in teeth irradiated in vivo(2017) GOMES-SILVA, Wagner; RIBEIRO, Ana Carolina Prado; CASTRO JUNIOR, Gilberto de; SALVAJOLI, Joao Victor; PALMIER, Natalia Rangel; LOPES, Marcio Ajudarte; ROCHA, Marcelo Marques; GOES, Mario Fernando de; BRANDAO, Thais Bianca; SANTOS-SILVA, Alan RogerObjective. Recent studies suggested that head and neck radiotherapy increases active forms of matrix metalloproteinases (MMPs) in the dentin-enamel junction (DEJ), leading to enamel delamination and radiation-related caries. This study aimed to assess the expression and activity of the gelatinases MMP-2 and MMP-9 in the DEJ and dentin-pulp complex tissues of teeth irradiated in vivo. Study Design. Thirty-six teeth were studied, including 19 irradiated and 17 non-irradiated controls. In situ zymography was used to investigate the gelatinolytic activity in the micromorphologic components of enamel, DEJ, dentin-pulp complex, and caries. Immunohistochemical analysis was conducted on the demineralized samples to assess MMP-2 and MMP-9 expression levels in the DEJ, dentin-pulp complex components, and caries. Results. No statistically significant differences were detected between groups in gelatinolytic activity or in MMP-2 expression levels (P > .05). Odontoblast MMP-9 expression was reduced in the irradiated group (P = .02). Conclusions. The study rejected the hypothesis that MMP-2 and MMP-9 would be overexpressed or more activated in the DEJ and dentin-pulp complex of irradiated teeth. Direct effects of radiation should not be regarded as an independent factor for explaining radiation-related caries onset and progression.
- Emergency changes in international guidelines on treatment for head and neck cancer patients during the COVID-19 pandemic(2020) CHAVES, Aline Lauda Freitas; CASTRO, Ana Ferreira; MARTA, Gustavo Nader; JUNIOR, Gilberto Castro; FERRIS, Robert L.; GIGLIO, Raul Eduardo; GOLUSINSKI, Wojciech; GORPHE, Philippe; HOSAL, Sefik; LEEMANS, C. Rene; MAGNE, Nicolas; MEHANNA, Hisham; MESIA, Ricard; NETTO, Eduardo; PSYRRI, Amanda; SACCO, Assuntina G.; SHAH, Jatin; SIMON, Christian; VERMORKEN, Jan B.; KOWALSKI, Luiz Paulo
- Micromorphology of the Dental Pulp Is Highly Preserved in Cancer Patients Who Underwent Head and Neck Radiotherapy(2014) FARIA, Karma Morais; BRANDAO, Thais Bianca; RIBEIRO, Ana Carolina Prado; VASCONCELLOS, Adriele Ferreira Gouvea; CARVALHO, Icaro Thiago de; ARRUDA, Fernando Freire de; CASTRO JUNIOR, Gilberto; GROSS, Vanessa Cristina; ALMEIDA, Oslei Paes; LOPES, Marcio Ajudarte; SANTOS-SILVA, Alan RogerIntroduction: Teeth are often included in the radiation field during head and neck radiotherapy, and recent clinical evidence suggests that dental pulp is negatively affected by the direct effects of radiation, leading to impaired sensitivity of the dental pulp: Therefore, this study aimed to investigate the direct effects of radiation on the microvasculature, innervation, and extracellular matrix of the dental pulp of patients who have undergone head and neck radiotherapy. Methods: Twenty-three samples of dental pulp from patients who finished head and neck radiotherapy were analyzed. Samples were histologically processed and stained with hematoxylin-eosin for morphologic evaluation of the microvasculature, innervation, and extracellular matrix. Subsequently, immunohistochemical analysis of proteins related to vascularization (CD34 and smooth muscle actin), innervation (S-100, NCAM/CD56, and neurofilament), and extracellular matrix (vimentin) of the dental pulp was performed. Results: The morphologic study identified preservation of the microvasculature, nerve bundles, and components of the extracellular matrix in all studied samples. The immunohistochemical analysis confirmed the morphologic findings and showed a normal pattern of expression for the studied proteins in all samples. Conclusions: Direct effects of radiotherapy are not able to generate morphologic changes in the microvasculature, innervation, and extracellular matrix components of the dental pulp in head and neck cancer patients.
- Molecular basis of oral squamous cell carcinoma in young patients: Is it any different from older patients?(2018) COSTA, Sara Ferreira dos Santos; BRENNAN, Peter A.; GOMEZ, Ricardo Santiago; FREGNANI, Eduardo Rodrigues; SANTOS-SILVA, Alan Roger; MARTINS, Manoela Domingues; CASTRO-JUNIOR, Gilberto de; RAHIMI, Siavash; FONSECA, Felipe PaivaOral squamous cell carcinoma (OSCC) is the commonest subtype of oral cancer, mainly affecting older patients. It used to be a rare disease among individuals younger than 40years, but recently increased incidences in this age group are being reported worldwide. The pathogenesis of OSCC affecting young patients remains controversial, and the well-known etiological factors for oral cancer, tobacco, and alcohol use are believed to play a minor role in the carcinogenesis of the neoplasm, suggesting that the etiology and the molecular basis of OSCC may differ between younger and older patients. Although several molecular markers and chromosomal abnormalities have been demonstrated to differ between both groups, most of the studies have failed to find significant differences. Moreover, divergent results have also been obtained regarding the presence of high-risk human papillomavirus infection in OSCC of young patients. Given these contradictory results and the limited methodological approaches of the majority of the studies, the exact difference between both age groups remains to be fully established. In this review, we evaluate the available data to establish the current evidence that might support the hypothesis that the molecular basis of OSCC in young patients (especially those under 40years) differ from the older patients.