MARIANGELA MACCHIONE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
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conferenceObject Effects Of Air Pollution On Estrogen And Dioxin/aryl Hydrocarbon Receptors Gene Expression In Nasal Epithelium Of Female/male Mice(2013) YOSHIZAKI, K.; BRITO, J.; FUZIWARA, C.; SANTOS, T.; KIMURA, E.; MAUAD, T.; SALDIVA, P.; MACCHIONE, M.conferenceObject Cigarette Smoke Associated To Diesel Exhaust Particles (dep) In Mice: Synergy Effects(2013) ARANTES, P. M. M.; TOLEDO, A. C.; ALMEIDA, F. M.; OLIVO, C. R.; MAGALHAES, R. M.; ANG, P. L.; FORNAZIEIRO, L. R.; MACCHIONE, M.; SALDIVA, P. H. N.; BISELLI, P. J. C.; LOPES, F. D. T. Q. S.; MARTINS, M. A.- pH in exhaled breath condensate and nasal lavage as a biomarker of air pollution-related inflammation in street traffic-controllers and office-workers(2013) LIMA, Thamires Marques de; KAZAMA, Cristiane Mayumi; KOCZULLA, Andreas Rembert; HIEMSTRA, Pieter S.; MACCHIONE, Mariangela; FERNANDES, Ana Luisa Godoy; SANTOS, Ubiratan de Paula; BUENO-GARCIA, Maria Lucia; ZANETTA, Dirce Maria; ANDRE, Carmen Diva Saldiva de; SALDIVA, Paulo Hilario Nascimento; NAKAGAWA, Naomi KondoOBJECTIVE: To utilize low-cost and simple methods to assess airway and lung inflammation biomarkers related to air pollution. METHODS: A total of 87 male, non-smoking, healthy subjects working as street traffic-controllers or office-workers were examined to determine carbon monoxide in exhaled breath and to measure the pH in nasal lavage fluid and exhaled breath condensate. Air pollution exposure was measured by particulate matter concentration, and data were obtained from fixed monitoring stations (8-h work intervals per day, during the 5 consecutive days prior to the study). RESULTS: Exhaled carbon monoxide was two-fold greater in traffic-controllers than in office-workers. The mean pH values were 8.12 in exhaled breath condensate and 7.99 in nasal lavage fluid in office-workers; these values were lower in traffic-controllers (7.80 and 7.30, respectively). Both groups presented similar cytokines concentrations in both substrates, however, IL-1 beta and IL-8 were elevated in nasal lavage fluid compared with exhaled breath condensate. The particulate matter concentration was greater at the workplace of traffic-controllers compared with that of office-workers. CONCLUSION: The pH values of nasal lavage fluid and exhaled breath condensate are important, robust, easy to measure and reproducible biomarkers that can be used to monitor occupational exposure to air pollution. Additionally, traffic-controllers are at an increased risk of airway and lung inflammation during their occupational activities compared with office-workers.
- Effects of Cigarette Smoking Intensity on the Mucociliary Clearance of Active Smokers(2013) XAVIER, Rafaella Fagundes; RAMOS, Dionei; ITO, Juliana Tiyaki; RODRIGUES, Fernanda Maria Machado; BERTOLINI, Giovana Navarro; MACCHIONE, Mariangela; TOLEDO, Alessandra Choqueta de; RAMOS, Ercy Mara CipuloBackground: Smoking impairs mucociliary clearance and increases respiratory infection frequency and severity in subjects with and without smoking-related chronic lung diseases. Objective: This study evaluated the effects of smoking intensity on mucociliary clearance in active smokers. Methods: Seventy-five active smokers were grouped into light (1-10 cigarettes/day; n = 14), moderate (11-20 cigarettes/day; n = 34) and heavy smokers (>= 21 cigarettes/day; n = 27) before starting a smoking cessation programme. Smoking behaviour, nicotine dependence, pulmonary function, carbon monoxide in exhaled air (exCO), carboxyhaemoglobin (COHb) and mucociliary clearance measured by the saccharin transit time (STT) test were all evaluated. An age-matched non-smoker group (n = 24) was assessed using the same tests. Results: Moderate (49 +/- 7 years) and heavy smokers (46 +/- 8 years) had higher STT (p = 0.0001), exCO (p < 0.0001) and COHb (p < 0.0001) levels compared with light smokers (51 +/- 15 years) and non-smokers (50 +/- 11 years). A positive correlation was observed between STT and exCO (r = 0.4; p < 0.0001), STT and cigarettes/day (r = 0.3, p = 0.02) and exCO and cigarettes/day (r = 0.3, p < 0.01). Conclusion: Smoking impairs mucociliary clearance and is associated with cigarette smoking intensity.