MARISA DOLHNIKOFF
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
15 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 15
- Anacardic Acids from Cashew Nuts Ameliorate Lung Damage Induced by Exposure to Diesel Exhaust Particles in Mice(2013) CARVALHO, Ana Laura Nicoletti; ANNONI, Raquel; TORRES, Larissa Helena Lobo; DURAO, Ana Carolina Cardoso Santos; SHIMADA, Ana Lucia Borges; ALMEIDA, Francine Maria; HEBEDA, Cristina Bichels; LOPES, Fernanda Degobbi Tenorio Quirino Santos; DOLHNIKOFF, Marisa; MARTINS, Milton Arruda; SILVA, Luiz Fernando Ferraz; FARSKY, Sandra Helena Poliselli; SALDIVA, Paulo Hilario Nascimento; ULRICH, Cornelia M.; OWEN, Robert W.; MARCOURAKIS, Tania; TREVISAN, Maria Teresa Salles; MAUAD, ThaisAnacardic acids from cashew nut shell liquid, a Brazilian natural substance, have antimicrobial and antioxidant activities and modulate immune responses and angiogenesis. As inflammatory lung diseases have been correlated to environmental pollutants exposure and no reports addressing the effects of dietary supplementation with anacardic acids on lung inflammation in vivo have been evidenced, we investigated the effects of supplementation with anacardic acids in a model of diesel exhaust particle-(DEP-) induced lung inflammation. BALB/c mice received an intranasal instillation of 50 mu g of DEP for 20 days. Ten days prior to DEP instillation, animals were pretreated orally with 50, 150, or 250mg/kg of anacardic acids or vehicle (100 mu L of cashew nut oil) for 30 days. The biomarkers of inflammatory and antioxidant responses in the alveolar parenchyma, bronchoalveolar lavage fluid (BALF), and pulmonary vessels were investigated. All doses of anacardic acids ameliorated antioxidant enzyme activities and decreased vascular adhesion molecule in vessels. Animals that received 50mg/kg of anacardic acids showed decreased levels of neutrophils and tumor necrosis factor in the lungs and BALF, respectively. In summary, we demonstrated that AAs supplementation has a potential protective role on oxidative and inflammatory mechanisms in the lungs.
conferenceObject Cd23+(fc epsilon rii - Low Affinity Ige Receptor) Cells In Large Airways And Bronchopulmonary Lymph Nodes Of Fatal Asthma(2013) CAGNONI, E. F.; FERREIRA, D. S.; SILVA, L. F. Ferraz da; CARVALHO, A. N.; SANTOS, A. B. G.; MEDEIROS, M. R.; DOLHNIKOFF, M.; RABE, K. F.; MAUAD, T.conferenceObject Elastic fibers and fibronectin expression in severe asthmatics(2013) FERREIRA, Diogenes; ANNONI, Raquel; CARVALHO-PINTO, Regina Maria; RABE, Klaus; CUKIER, Alberto; STELMACH, Rafael; DOLHNIKOFF, Marisa; MAUAD, Thais- Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats(2013) SAAD, Karen Ruggeri; SAAD, Paulo Fernandes; DANTAS FILHO, Luiz; BRITO, Jose Mara de; KOIKE, Marcia Kiyomi; ZANONI, Fernando Luiz; DOLHNIKOFF, Marisa; MONTERO, Edna Frasson de SouzaBackground: Experimental hemorrhagic shock (HS) is based on controlling bleeding and the treatment of fluid resuscitation to restore tissue oxygenation and perfusion. The HS could promote ischemia/reperfusion injury, which induces a general exacerbation of the inflammatory process, initially compromising the lungs. N-acetylcysteine (NAC), an antioxidant, may attenuate ischemia/reperfusion injury. This study evaluated the effect of NAC in association with fluid resuscitation on pulmonary injury in a controlled HS model in rats. Methods: Male Wistar rats were submitted to controlled HS (mean arterial pressure of 35 mm Hg for 60 min). Two groups were constituted according to resuscitation solution administered: RLG (Ringer's lactate solution) and RLG+NAC (Ringer's lactate in association with 150 mg/kg NAC. A control group was submitted to catheterization only. After 120 min of resuscitation, bronchoalveolar lavage was performed to assess intra-alveolar cell infiltration and pulmonary tissue was collected for assessment of malondialdehyde, interleukin 6, and interleukin 10 and histopathology. Results: Compared with the RLG group, the RLG+NAC group showed lower bronchoalveolar lavage inflammatory cell numbers, lower interstitial inflammatory infiltration in pulmonary parenchyma, and lower malondialdehyde concentration. However, tissue cytokine (interleukin 6 and interleukin 10) expression levels were similar. Conclusion: N-acetylcysteine was associated with fluid resuscitationeattenuated oxidative stress and inflammatory cell infiltration in pulmonary parenchyma. N-acetylcysteine did not modify cytokine expression.
conferenceObject Influenza A (H1N1) Virus Infection Is Associated With Increased Number Of Cd8t+Lymphocytes And Cd83+Dendritic Cells In Lung Tissue(2013) BUTTIGNOL, M.; PIRES-NETO, R. C.; BALLARIN, M.; RODIO, G.; DOLHNIKOFF, M.; MAUAD, T.- Airway Dimensions in Fatal Asthma and Fatal COPD: Overlap in Older Patients(2013) SENHORINI, Aletea; FERREIRA, Diogenes S.; SHIANG, Christina; SILVA, Luiz F. F.; DOLHNIKOFF, Marisa; GELB, Arthur F.; MAUAD, ThaisIn some patients with chronic asthma clinical and physiological similarities with COPD may exist, such as partial reversibility to bronchodilators and persistent expiratory airflow obstruction. However, pathological data comparing both diseases in patients of similar age and disease severity are scarce. We compared large and small airway dimensions in 12 younger (mean age 32 yrs) and 15 older (mean age 65 yrs) non-smoker adult fatal asthma patients with 14 chronic smokers with severe, fatal COPD (mean age 71 yrs). Using H&E, Movat pentachrome staining and image analysis, we quantified large airway basement membrane (BM) thickness (mu m), submucosal gland area and large and small airway inner wall, smooth muscle and outer wall areas. Areas were normalized by BM perimeter (mu m(2)/mu m). Younger adult fatal asthma patients had thicker BM, smooth muscle, and outer wall areas in both small and large airways when compared to COPD patients. In older asthmatics there was an overlap in BM thickness and airway structure in small airways. Inner wall layer in large and small airway level and submucosal gland areas were similar among groups. In conclusion, there are airway histological structural similarities between fatal asthma and fatal COPD. Older fatal asthmatics present overlapping airway structural features with younger adult fatal asthmatics and severe COPD patients. Our data contributes to a better understanding of asthma pathology in the elderly.
- Minimally Invasive Adenocarcinoma of the Lung in a Young Patient Treated for Osteosarcoma(2013) BERNARDI, Fabiola Del Carlo; GARCIA, Julia Lopes; ALMEIDA, Maria Tereza Assis de; ZAMPERLINI, Gustavo; SOARES, Ibere Cauduro; ODONE FILHO, Vicente; DOLHNIKOFF, Marisa; MAUAD, ThaisAlthough children with osteosarcoma have a higher incidence of a 2nd malignancy than the general population, its development in the lung is rare. The few reported cases belong to examples of carcinomas. Here we present the case of a 13-year-old boy with a primary pulmonary adenocarcinoma diagnosed 3 years after the osteosarcoma diagnosis and present a review of the literature.
conferenceObject Granzyme A Expression In Fatal Asthma(2013) SILVA, L. F.; SANTOS, F. E.; ANNONI, R.; NUSSBAUMER-OCHSNER, Y.; DOLHNIKOFF, M.; RABE, K. F.; MAUAD, T.; HIEMSTRA, P.conferenceObject Collagens And Airway Function In Severe Asthma(2013) FERREIRA, D. S.; PINTO, R. M. C.; OLIVEIRA, B. L.; FRARI, H. S. Del; RABE, K. F.; CUKIER, A.; STELMACH, R.; DOLHNIKOFF, M.; MAUAD, T.conferenceObject Expression of CD1a cells and VCAM in large airways and thoracic lymph nodes of fatal asthma(2013) CAGNONI, Erika; FERREIRA, Diogenes; SILVA, Luiz Fernando; CARVALHO, Ana Laura; SANTOS, Angela; MEDEIROS, Maria Cristina; DOLHNIKOFF, Marisa; RABE, Klauss; MAUAD, Thais