MARISA DOLHNIKOFF
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
8 resultados
Resultados de Busca
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conferenceObject IMPACTS OF RECREATIONAL USE OF CANNABIS SATIVA ON GESTATIONAL AND DEVELOPMENTAL OUTCOMES IN MICE: PRELIMINARY RESULTS(2015) BENEVENUTO, S. G. M.; BELOTTI, L.; COSTA, N. S. X.; DOLHNIKOFF, M.; RIBEIRO JUNIOR, G.; LOURENCO, D. T.; VERAS, M. M.- Linfadenomegalia e febre em chefe de cozinha durante viagem à Europa(2015) KAWANO-DOURADO, Leticia; PEIRERA, Daniel Antunes Silva; KAWASSAKI, Alexandre de Melo; DOLHNIKOFF, Marisa; SILVA, Marcos Vinicius da; KAIRALLA, Ronaldo AdibThis case illustrates a rare presentation (as lymphadenopathy and fever) of one of the most common zoonotic diseases worldwide brucellosis in a 22-year-old Brazilian male (a chef) who had recently returned to Brazil after having lived in and traveled around Europe for one year. The histopathology, clinical history, and response to treatment were all consistent with a diagnosis of brucellosis, which was confirmed by PCR in a urine sample. We also review some aspects of brucellosis, such as the clinical features, diagnosis, and management.
- T and B lymphocytes, NK-cells, dendritic cells, mast cells and foxp3+cells in lung tissue of severe Influenza A (H1N1) pdm infection(2015) BUTTIGNOL, Monique; PIRES-NETO, Ruy; BALLARIN, Marina; DOLHNIKOFF, Marisa; MAUAD, Thais
- Chemical composition modulates the adverse effects of particles on the mucociliary epithelium(2015) CARVALHO-OLIVEIRA, Regiani; PIRES-NETO, Ruy Camargo; BUSTILLOS, Jose Oscar Viega; MACCHIONE, Mariangela; DOLHNIKOFF, Marisa; SALDIVA, Paulo H. Nascimento; GARCIA, Maria Lucia BuenoOBJECTIVE: We compared the adverse effects of two types of real ambient particles; i.e., total suspended particles from an electrostatic precipitator of a steel mill and fine air particles from an urban ambient particulate matter of 2.5 mu m, on mucociliary clearance. METHOD: Mucociliary function was quantified by mucociliary transport, ciliary beating frequency and the amount of acid and neutral mucous in epithelial cells through morphometry of frog palate preparations. The palates were immersed in one of the following solutions: total suspended particles (0.1 mg/mL), particulate matter 2.5 mm 0.1 mg/mL (PM0.1) or 3.0 mg/mL (PM3.0) and amphibian Ringer's solution (control). Particle chemical compositions were determined by X-ray fluorescence and gas chromatography/mass spectrometry. RESULTS: Exposure to total suspended particles and PM3.0 decreased mucociliary transport. Ciliary beating frequency was diminished by total suspended particles at all times during exposure, while particulate matter of 2.5 mm did not elicit changes. Particulate matter of 2.5 mm reduced epithelial mucous and epithelium thickness, while total suspended particles behaved similarly to the control group. Total suspended particles exhibited a predominance of Fe and no organic compounds, while the particulate matter 2.5 mm contained predominant amounts of S, Fe, Si and, to a lesser extent, Cu, Ni, V, Zn and organic compounds. CONCLUSION: Our results showed that different compositions of particles induced different airway epithelial responses, emphasizing that knowledge of their individual characteristics may help to establish policies aimed at controlling air pollution.
- Aerobic exercise reduces lung inflammation and fibrosis mediated by serotoninergic/Akt signaling in a model of pulmonary fibrosis(2015) VIEIRA, Rodolfo P.; ALMEIDA-OLIVEIRA, Ana Roberta; PEREIRA, Paulo R.; MATOS, Yves; OLIVEIRA-JUNIOR, Manoel C.; GREIFFO, Flavia R.; RIGONATO-OLIVEIRA, Nicole C.; ANDRADE-SOUSA, Adilson S.; BRUGEMMAN, Thayse R.; DELLE, Humberto; ALBERTINI, Regiane; OLIVEIRA, Ana Paula L. De; DAMACENO-RODRIGUES, Nilsa R.; FERNANDEZ, Isis E.; DOLHNIKOFF, Marisa; CASTRO-FARIA-NETO, Hugo C.; IDZKO, Marco; EICKELBERG, Oliver
conferenceObject Eosinophilic Bronchiolitis And Asthmatic Granulomatosis: A Case Report Showing A Spectrum Of Presentation(2015) ARIMURA, F. E.; FERREIRA, B. C. P.; FELISBINO, M. B.; ATHANAZIO, R. A.; RACHED, S. Z.; BALDI, B. G.; NASCIMENTO, E. C. T. D.; MAUAD, T.; CARVALHO-PINTO, R. M. D.; DOLHNIKOFF, M.; STELMACH, R.; CUKIER, A.- Bronchopulmonary lymph nodes and large airway cell trafficking in patients with fatal asthma(2015) CAGNONI, Erika Feltrini; FERREIRA, Diogenes Seraphim; SILVA, Luiz Fernando Ferraz da; PETRY, Ana Laura Nicoletti Carvalho; SANTOS, Angela Batista Gomes dos; MEDEIROS, Maria Cristina Rodrigues; DOLHNIKOFF, Marisa; RABE, Klaus F.; MAUAD, ThaisBackground: Immune responses in asthmatic patients involve coordinated cellular responses in the airways and lymph nodes (LNs). However, no studies have described the composition of different cell populations in the bronchopulmonary LNs of asthmatic patients. Objective: We sought to investigate the expression of dendritic cells (DCs) and costimulatory molecules, B cells, T cells, T(H)2-related cytokines, eosinophils, and vascular cell adhesion molecule in the bronchopulmonary LNs and large airways of asthmatic patients. Methods: Using histochemistry, immunohistochemistry, and image analysis, we investigated the expression of Factor XIIIa(+), CD1a(+), CD83(+), and CD207(+) DCs; CD4(+) and CD8(+) T cells; CD20(+) B cells; CD23(+) (Fc epsilon RII) cells; IL-4; IL-5; eosinophils, and vascular cell adhesion molecule 1 in the large airways and bronchopulmonary LNs of 11 nonsmokers who died from an asthma exacerbation (fatal asthma [FA]) in comparison with 8 nonasthmatic control subjects. In selected cases of FA, we analyzed the coexpression of HLA-DR, CD40, and CD80 in lung and LN eosinophils. Results: The LNs of asthmatic patients exhibited increased density of eosinophils. No other cells were expressed differently in the LNs of patients with FA. The large airways of patients with FA had increased expression of eosinophils in all layers and increased expression of Factor XIIIa(+) cells, CD4(+) and CD8(+) T cells, CD20(+) B cells, and CD23(+) cells in the outer layer. There was colocalization of HLA-DR, CD40, and CD80 in the eosinophils at both sites. Conclusions: FA is associated with the increased presence of eosinophils in the LNs and large airways, which express HLA-DR and costimulatory molecules. The expression of Factor XIIIa(+) monocyte-derived DCs, CD4(+) and CD8(+) T cells, CD20(+) B cells, and CD23(+) cells was increased in the large airways without a corresponding increase in the expression of these cells in the bronchopulmonary LNs. These findings support the concept that eosinophils might act as antigen-presenting cells in patients with FA.
- Versican and collagen-III expression in bronchial and pulmonary muscular arteries in COPD patients(2015) ANNONI, Raquel; COUCEIRO, Ligia; BARBOSA, Silvio; LANCAS, Tatiana; BATTAGLIA, Salvatore; DOLHNIKOFF, Marisa; HIEMSTRA, Pieter; STERK, Peter; RABE, Klaus; MAUAD, Thais