ANA CAROLINA PRADO RIBEIRO E SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
5 resultados
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- The impact of head and neck radiotherapy on the dentine- enamel junction: a systematic review(2020) FONSECA, Jessica Montenegro; TROCONIS, Cristhian Camilo Madrid; PALMIER, Natalia Rangel; GOMES-SILVA, Wagner; PAGLIONI, Mariana de Pauli; ARAUJO, Anna Luiza Damaceno; ARBO-LEDA, Lady Paola Aristizabal; VECHIATO FILHO, Aljomar Jose; GONZALEZ-ARRIAGADA, Wilfredo Alejandro; GOES, Mario Fernando de; LOPES, Marcio Ajudarte; BRANDAO, Thais Bianca; VARGAS, Pablo Agustin; RIBEIRO, Ana Carolina Prado; SANTOS-SILVA, Alan RogerBackground: Radiotherapy is widely used in contemporary head and neck cancer treatment protocols. The ability of head and neck radiotherapy (HNRT) to cause direct radiogenic destruction to the teeth is one of the most controversial topics in the field of oral oncology. Therefore, this systematic review aimed to investigate ionising radiation as an independent factor for physical and chemical changes on the dentine-enamel junction (DEJ), a pivotal dental topography for the onset and progression of radiation-related caries (RRC) and enamel delamination. Material and Methods: Systematic searches were conducted on three databases: Scopus, MEDLINE (Via PubMed) and Embase (Elsevier). Laboratory studies evaluating the effects of simulated or in vivo HNRT on the DEJ were included. The GRADE tool adapted for in vitro studies was used to assess the methodological quality. Results: Of the 154 initially selected studies, eight met the inclusion criteria, from which five studies were graded as high quality of evidence, two studies were graded as moderate quality and one as low quality. Two studies did not demonstrate DEJ alterations following HNRT while the other six articles described several organic and inorganic changes in the DEJ of irradiated teeth samples. These radiogenic events were mostly detected through micro and nanoindentation, Raman micro-spectroscopy, confocal microscopy, Western blotting and optical coherence tomography. Conclusions: HNRT may have a negative impact on the physical and chemical aspects of the DEJ, predisposing cancer patients to RRC and enamel delamination.
- Different methods of cell quantification can lead to different results: a comparison of digital methods using a pilot study of dendritic cells in HIV-positive patients(2020) FERNANDES, Diego Tetzner; HEERDEN, Willie F. P. van; RIBEIRO, Ana Carolina Prado; BRANDAO, Thais Bianca; MELLO, Evandro Sobroza de; RIVERA, Cesar; HEERDEN, Marlene B. van; GONDAK, Rogerio; SANTOS-SILVA, Alan Roger; VARGAS, Pablo Agustin; LOPES, Marcio AjudarteBackground: Although new digital pathology tools have improved the positive cell quantification, there is a heterogeneity of the quantification methods in the literature. The aim of this study was to evaluate and propose a novel dendritic cells quantification method in squamous cell carcinoma comparing it with a conventional quantification method. Material and Methods: Twenty-six squamous cell carcinomas HIV-positive cases affecting the oropharynx, lips and oral cavity were selected. Immunohistochemistry for CD1a, CD83, and CD207 was performed. The immunohistochemical stains were evaluated by automated examination using a positive pixel count algorithm. A conventional quantification method (unspecific area method; UA) and a novel method (specific area method; SA) were performed obtaining the corresponding density of positive dendritic cells for the intratumoral and peritumoral regions. The Mann-Whitney U test was used to verify the influence of the quantification methods on the positive cell counting according to the evaluated regions. Data were subjected to the ANOVA and Student's t-test to verify the influence of the tumour location, stage, histological grade, and amount of inflammation on the dendritic cells density counting. Results: The cell quantification method affected the dendritic cells counting independently of the evaluated region (P-value < 0.05). Significant differences between methods were also observed according to the tumour features evaluations. Conclusions: The positive cell quantification method influences the dendritic cells density results. Unlike the conventional method (UA method), the novel SA method avoids non-target areas included in the hotspots improving the reliability and reproducibility of the density cell quantification.
- HIV-related non-Hodgkin lymphomas affecting the oral cavity: a clinicopathologic study of 11 cases(2022) UCHOA, Daniel Cavallero Colares; PONTES, Flavia Sirotheau Correa; SOUZA, Lucas Lacerda de; SANTOS, Gabriela Sepeda dos; PRADO-RIBEIRO, Ana Carolina; BRANDAO, Thais Bianca; OLIVEIRA, Leticia Rodrigues de; GOMES, Carolina Cavalieri; SANTOS-SILVA, Alan Roger; FONSECA, Felipe Paiva; ALMEIDA, Oslei Paes de; PONTES, Helder Antonio RebeloBackground: HIV-related non-Hodgkin lymphomas of the oral cavity are rare lesions with aggressive clinical behaviour. The aim of this study is to describe the clinicopathological features of a series of HIV-related oral non Hodgkin lymphomas. Material and Methods: Eleven cases of oral lymphomas affecting HIV-positive patients were retrieved from 2012 to 2019. Clinicopathological features regarding age, sex, tumour location, clinical presentation, laboratory findings, disease stage and follow-up were obtained. Histologic, immunohistochemical and in situ hybridization for EBV detection were done for diagnosis confirmation. Overall survival was estimated by Kaplan-Meier curve. Results: Males predominated, with a mean age of 40.3 years-old. Maxilla and mandible were the mostly affected. Plasmablastic lymphoma and diffuse large B-cell lymphoma not otherwise specified (NOS) were the main histological types. Lesions presented as reddish ulcerated swellings, representing the first sign of AIDS in six cases. Stage IV were common (7 cases) and the mean HIV viral load was 10,557 copies/mL, with a mean of 266 CD4+ cells/mm3, 1,278 CD8+ cells/mm3 and a CD4+/CD8+ ratio of 0.26. Eight patients died of the disease (72.7%). Overall survival revealed that 78.2% of the patients died after 21 months of follow-up. Conclusions: HIV-related oral lymphomas present a poor prognosis usually diagnosed in advanced stages and in our series plasmablastic lymphoma was the most common subtype.
- Oral Kaposi sarcoma development is associated with HIV viral load, CD4+count and CD4+/CD8+ratio(2021) SOUSA, Rosa Hiolanda Abreu de; SOUZA, Lucas Lacerda de; GUEDES, Pablyanne Tereza Louzada; PRADO-RIBEIRO, Ana Carolina; RODRIGUES-OLIVEIRA, Leticia; BRANDAO, Thais Bianca; ALVES, Barbara Waleria Goncalves; LOPES, Marcio Ajudarte; SANTOS-SILVA, Alan; MONTEIRO, Julius Caesar Mendes Soares; GONCALVES, Thais Tapajos; ALMEIDA, Oslei Paes de; PONTES, Flavia Sirotheau Correa; PONTES, Helder Antonio RebeloBackground: Kaposi's sarcoma (KS) is an uncommon, multifocal and angioproliferative lesion, which demonstrates a poor prognosis. The aim of the present research was to explore the association of HIV viral load, CD4+ and CD8+ counts and the CD4+/CD8+ ratio on the risk of oral Kaposi's sarcoma (KS) development. Material and Methods: A total of 62 patients were retrieved from March 2008 to October 2020 from the files of two oral pathology centres. Clinical, laboratory and follow-up data were retrieved from their medical files. Poisson regression was used to explore the role of history of immunosuppression and its association with oral KS development. A P-value <0.05 was considered significant. Results: Sixty-two patients were included in the present study (32 with oral KS and 30 with no presentation of lesions anywhere on the body). Patients with oral KS presented a mean age of 32.6 years, and male patients were more affected. The hard palate (15 cases; 46.8%) was the main anatomical site affected. The lesions were mostly presented as swellings (13 cases; 40.6%) and nodules (12 cases; 37.5%). Systemic manifestations were also observed, including candidiasis (4 cases; 12.5%), bacterial infection (3 cases; 9.3%), tuberculosis (3 cases; 9.3%), herpes simplex (3 cases; 9.3%) and pneumonia (3 cases; 9.3%). A significant correlation was observed between HIV viral load, CD4+ count and the CD4+/CD8+ ratio with oral KS development Conclusions: HIV viral load, CD4+ count and the CD4+/CD8+ ratio are associated with oral KS development.
- Radiation-related superficial oral mucoceles: An under-recognized acute toxicity in head and neck cancer patients(2018) PRADO-RIBEIRO, Ana-Carolina; SANTOS-SILVA, Alan-Roger; FARIA, Karina-Morais; SILVA, Wagner-Gomes; SIMONATO, Luciana-Estevam; MOUTINHO, Karina; BRANDAO, Thais-BiancaBackground: Acute toxicity is usually defined as adverse changes occurring immediately or a short time after the start of oncological treatment. Material an Methods: Cross-sectional retrospective study performed with head and neck cancer patients who underwent radiotherapy from 2013 to 2016. Results: Ten (1.2%) patients developed SOMs during radiotherapy, most (80%) of which were men with a mean age of 59.5 years at diagnosis. SOMs mainly affected the floor of the mouth (60%) between the fourth and the sixth weeks of radiation therapy. All lesions were asymptomatic and spontaneously ruptured approximately 9 days after diagnosis. Conclusions: Although rare, SOMs may be regarded as an acute oral toxicity of head and neck radiotherapy.