DANIEL ROMERO MUñOZ
Projetos de Pesquisa
Unidades Organizacionais
Departamento de MedicinaLegal, Ética Médica e Medicina Social e do Trabalho, Faculdade de Medicina - Docente
LIM/40 - Laboratório de Imunohematologia e Hematologia Forense, Hospital das Clínicas, Faculdade de Medicina
LIM/40 - Laboratório de Imunohematologia e Hematologia Forense, Hospital das Clínicas, Faculdade de Medicina
24 resultados
Resultados de Busca
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- Determination of antidepressants in whole blood using hollow-fiber liquid-phase microextraction and gas chromatography-mass spectrometry(2014) SANTOS, Marcelo Filonzi dos; FERRI, Caio Caleiras; SEULIN, Saskia Carolina; LEYTON, Vilma; PASQUALUCCI, Carlos Augusto Goncalves; MUNOZ, Daniel Romero; YONAMINE, MauricioA hollow-fiber liquid-phase microextraction (HF-LPME), used in three-phase mode, and combined with gas chromatography-mass spectrometry (GC-MS), was developed to quantify antidepressants and their major metabolites (amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, desmethylclomipramine, fluoxetine, and norfluoxetine) in whole blood samples, using their deuterated analogs as internal standards. The HF-LPME system comprised a disposable 8-cm polypropylene porous hollow fiber, 4.0 ml of sample solution (0.5 ml of blood added to 3.5 ml of 0.1 M NaOH: donor phase), dodecane (organic phase), and 0.1 M formic acid (acceptor phase) for extraction. After stirring the system, the acceptor phase was evaporated under a nitrogen stream and resuspended in 30 mu l of methanol. Derivatization was not required. A 2.0-mu l aliquot of this solution was injected into a GC-MS system. The method was validated after the optimization of several parameters that may influence the extraction efficiency. The limits of quantification for all antidepressants were below the therapeutic levels (20.0 ng/ml). The average intraday and interday precisions were within 9.7 and 9.8 %, respectively, for all analytes. The calibration curves were linear in the concentration range of 20-1,200 ng/ml. The developed method was applied to seven actual postmortem samples. Tricyclic antidepressants were detected in all of the analyzed cases. To our knowledge, this is the first demonstration of usefulness of HF-LPME for analysis of antidepressants in postmortem forensic cases.
- A continuidade do uso de anfetaminas por motoristas de caminhão no Estado de São Paulo, Brasil, a despeito da proibição de sua produção, prescrição e uso(2013) OLIVEIRA, Lucio Garcia de; ENDO, Ligia Goes; SINAGAWA, Daniele Mayumi; YONAMINE, Mauricio; MUNOZ, Daniel Romero; LEYTON, VilmaAmphetamine use by truck drivers for occupational purposes is widely known. The production and consumption of amphetamines was banned by the Brazilian National Health Surveillance Agency (ANVISA) in October 2011. This study analyzes persistent amphetamine use by truck drivers since the ban was implemented. A convenience sample of 427 truck drivers was taken along highways in Sao Paulo State in 2012. Participants were asked to answer a structured questionnaire and provide a urine sample to screen for recent amphetamine consumption through toxicological analysis. Among the interviewed drivers, 7% had used some illicit drug recently and 2.7% had used amphetamines. Amphetamines are still consumed by truck drivers despite the risks and the recent ban. The authorities should thus monitor the possession and use of amphetamines by drivers in order to effectively enforce the ban.
- Alcohol use among fatally injured victims in SAo Paulo, Brazil: bridging the gap between research and health services in developing countries(2017) ANDREUCCETTI, Gabriel; LEYTON, Vilma; LEMOS, Nikolas P.; MIZIARA, Ivan Dieb; YE, Yu; TAKITANE, Juliana; MUNOZ, Daniel Romero; REINGOLD, Arthur L.; CHERPITEL, Cheryl J.; CARVALHO, Heraclito Barbosa deBackground and aimsMost studies reporting alcohol use among fatally injured victims are subject to bias, particularly those related to sample selection and to absence of injury context data. We developed a research method to estimate the prevalence of alcohol consumption and test correlates of alcohol use prior to fatal injuries. Design, Setting and ParticipantsCross-sectional study based on a probability sample of fatally injured adult victims (n=365) autopsied in SAo Paulo, Brazil. Victims were sampled within systematically selected 8-hour sampling blocks, generating a representative sample of fatal injuries occurring during all hours of the day for each day of the week between June 2014 and December 2015. MeasurementsThe presence of alcohol and blood alcohol concentration (BAC) were the primary outcomes evaluated according to victims' socio-demographic, injury context data (type, day, time and injury place) and criminal history characteristics. FindingsAlcohol was detected in 30.1% [95% confidence interval (CI)=25.6-35.1)] of the victims, with a mean blood alcohol level (BAC) level of 0.11%w/v (95% CI=0.09-0.13) among alcohol-positive cases. Black and mixed race victims presented a higher mean BAC than white victims (P=0.03). Fewer than one in every six suicides tested positive for alcohol, while almost half of traffic-related casualties were alcohol-positive. Having suffered traffic-related injuries, particularly those involving vehicle crashes, and injuries occurring during weekends and at night were associated significantly with alcohol use before injury (P<0.05). ConclusionsNearly one-third of fatal injuries in SAo Paulo between June 2014 and December 2015 were alcohol-related, with traffic accidents showing a greater association with alcohol use than other injuries. The sampling methodology tested here, including the possibility of adding injury context data to improve population-based estimates of alcohol use before fatal injury, appears to be a reliable and lower-cost strategy for avoiding biases common in death investigations.
- Prevalence of drugs in oral fluid from truck drivers in Brazilian highways(2017) BOMBANA, Henrique Silva; GJERDE, Hallvard; SANTOS, Marcelo Filonzi dos; JAMT, Ragnhild Elen Gjulem; YONAMINE, Mauricio; ROHLFS, Waldo Jose Caram; MUNOZ, Daniel Romero; LEYTON, VilmaTraffic accidents are responsible for 1.25 million deaths worldwide and are the most common cause of death among those aged 15-29 years. In Brazil, traffic accidents caused more than 44,000 deaths in 2014. The use of psychoactive drugs is an important risk factor for being involved in traffic accidents. Previous studies have found that psychoactive substances are commonly used by truck drivers in Brazil to maintain their extensive work schedule and stay awake while driving during nighttime hours. The state of Sao Paulo is one of the most important states regarding goods transportation. Important highways cross through Sao Paulo to other regions from Brazil and to other countries in Latin America. This study aims to determine the prevalence of illicit drug use by truck drivers in the state of Sao Paulo through toxicological analyses of oral fluid. Truck drivers were randomly stopped by police officers on federal roads during morning hours. Oral fluid samples were collected using the Quantisal (TM) device. In addition, a questionnaire concerning sociodemographic characteristics and health information was administered. Oral fluid samples were screened for amphetamine, cocaine, and tetrahydrocannabinol (Delta 9-THC) by ELISA and the confirmation was performed using ultra performance liquid chromatography with tandem mass spectrometry detection (UPLC-MS/MS). Of the 764 drivers stopped, 762 agreed to participate. The participants were driving an average of 614 km and 9.4 h a day. Of the total samples, 5.2% (n = 40) tested positive for drugs. Cocaine was the most frequently found drug (n = 21), followed by amphetamine (n = 16) and D9-THC (n = 8). All drivers were men with an average age of 42.5 years. With these results we were able to verify that many truck drivers were still consuming psychoactive drugs while driving, and cocaine was the most prevalent one. This reinforces the need for preventive measures aimed at controlling the use of illicit drugs by truck drivers in Brazil.
- Prevalence of at-risk drinking among Brazilian truck drivers and its interference on the performance of executive cognitive tasks(2016) OLIVEIRA, Lucio Garcia de; LEOPOLDO, Kae; GOUVEA, Marcela Julio Cesar; BARROSO, Lucia Pereira; GOUVEI, Paula Adriana Rodrigues; MUNOZ, Daniel Romero; LEYTON, VilmaBackground: Binge drinking (BD) has been associated with an increase in the risk of alcohol-related injuries. Alcohol continues to be the main substance consumed by truck drivers, a population of special concern, since they are often involved in traffic accidents. The aim of this study was to estimate the prevalence of BD and its interference in the executive functioning among truck drivers in Sao Paulo, Brazil. Methods: A non-probabilistic sample of 684 truck drivers was requested to answer a structured research instrument on their demographic data and alcohol use. They performed cognitive tests to assess their executive functioning and inventories about confounding variables. The participants were then divided according to their involvement in BD. Results: 17.5% of the interviewees have reported being engaged in BD. Binge drinkers showed a better performance on one test, despite having done so at the expense of more mistakes and lower accuracy. More interestingly, binge drinkers took three seconds longer than non-binge drinkers to inhibit an inadequate response, which is worrisome in the context of traffic. Overall, the deleterious effect of BD on performance remained after controlling for the effects of confounding variables in regression logistic models. Conclusions: As the use of alcohol among truck drivers may be as a way to get by with their work conditions, we believe that a negotiation between their work organization and public authorities would reduce such use, preventing negative interferences on truck drivers' cognitive functioning, which by its turn may also prevent traffic accidents.
- Uso de anfetaminas por motoristas de caminhão em rodovias do Estado de São Paulo: um risco à ocorrência de acidentes de trânsito?(2013) TAKITANE, Juliana; OLIVEIRA, Lucio Garcia de; ENDO, Ligia Goes; OLIVEIRA, Keziah Cristina Barbosa Gruber de; MUNOZ, Daniel Romero; YONAMINE, Mauricio; LEYTON, VilmaThe use of amphetamines in Brazil is common among truck drivers, which may be an important factor in the occurrence of traffic accidents. This article seeks to estimate the prevalence of amphetamine use among truck drivers. Drivers (N = 134) were stopped on two different highways in Sao Paulo state and they were asked to answer a questionnaire and provide a urine sample for toxicological analysis. All data were analyzed on Stata 8.0. All participants were males with low levels of schooling, whose mean age was 40.8 years. The presence of amphetamines was detected in 10.8% of all urine samples collected, being commonly justified in order to make truck drivers able to maintain their state of awareness. Amphetamine use was detected among truck drivers on Sao Paulo highways. The problem is that when the stimulant effects wear off, sleepiness due to sleep deprivation reduces concentration and good driver performance, making drivers vulnerable to traffic accidents and the related effects.
- Determination of Opiates in Whole Blood and Vitreous Humor: A Study of the Matrix Effect and an Experimental Design to Optimize Conditions for the Enzymatic Hydrolysis of Glucuronides(2012) SANCHES, Livia Rentas; SEULIN, Saskia Carolina; LEYTON, Vilma; PARANHOS, Beatriz Aparecida Passos Bismara; PASQUALUCCI, Carlos Augusto; MUNOZ, Daniel Romero; OSSELTON, Michael David; YONAMINE, Mauricio
- Blood alcohol levels in suicide by hanging cases in the state of Sao Paulo, Brazil(2012) ZERBINI, Talita; PONCE, Julio de Carvalho; SINAGAWA, Daniele Mayumi; CINTRA, Raquel Barbosa; MUNOZ, Daniel Romero; LEYTON, VilmaSuicide is one of the main causes of violent death worldwide, and has become a public health issue. Since alcohol consumption is associated with the increase in the number of suicides and hanging is one of the main methods used worldwide, the present study consists of an epidemiological analysis of BACs in victims of suicide by hanging autopsied in the State of Sao Paulo, Brazil. The objective of the present work was to establish an epidemiological profile and evaluate blood alcohol concentrations in victims of suicide by hanging in the State of Sao Paulo, Brazil, in the year of 2007. A cross-sectional retrospective study was conducted by collection of secondary data from autopsy reports of victims of hanging. According to the present study, positive results for alcohol were higher in male victims, but the mean BAC was higher in women.
- Acidentes de trânsito envolvendo motoristas de caminhão no Estado de São Paulo: prevalência e preditores(2016) OLIVEIRA, Lucio Garcia de; ALMEIDA, Carlos Vinícius Dias de; BARROSO, Lucia Pereira; GOUVEA, Marcela Julio Cesar; MUñOZ, Daniel Romero; LEYTON, VilmaAbstract Abstract The mortality rate of traffic accidents (TA) is high in Brazil. Trucks are the second category of motor vehicles most often involved in TA. However, few studies have addressed the issue of TA among these professionals. The study aimed to estimate the prevalence of TA and their predictors in a sample of 684 truck drivers recruited in the state of São Paulo during 2012 and 2013. We requested participants to answer a research instrument on their personal and occupational data and their involvement in TA and traffic violations. A logistic regression model was developed to identify TA predictors. Almost 11% of the respondents suffered at least one TA in that timeframe. We identified the following TA predictors: having few years of experience as professional drivers (OR = 1.86; CI 95% = 1.05-3.38; p = 0.036); receiving some traffic tickets (OR = 1.91; CI 95% = 1.04-3.66; p = 0.043) and working more than 12 hours daily (OR = 1.84; CI 95% = 1.04-3.24; p = 0.034). Given those results, we suggest the development of a joint action among all the involved social stakeholders in order to negotiate truck drivers’ work organization aiming at reducing behaviors that may lead to traffic accidents.
- Introducing a new research method for evaluating alcohol and other drug use among fatally injured victims in Latin America(2017) ANDREUCCETTI, Gabriel; LEYTON, Vilma; MIZIARA, Ivan Dieb; LEMOS, Nikolas P.; MUNOZ, Daniel Romero; CHERPITEL, Cheryl J.; CARVALHO, Heraclito Barbosa
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