VERA LUCIA TEIXEIRA DE FREITAS
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Moléstias Infecciosas e Parasitárias, Faculdade de Medicina
LIM/48 - Laboratório de Imunologia, Hospital das Clínicas, Faculdade de Medicina
LIM/48 - Laboratório de Imunologia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
conferenceObject Interferon c and interleukin-12p40 genetic polymorphisms in brazilian patients with paracoccidioidomycosis(2012) HOLANDA, F. M. C.; SATO, P. K.; FREITAS, V. L. T.; YASUDA, M. A. ShikanaiParacoccidioidomycosis, caused by the thermal dimorphic fungus Paracoccidioides brasiliensis, is a chronic systemic granulomatous disease, endemic in South America, mainly in Brazil, being the eighth leading cause of death among infectious and parasitic diseases. Paracoccidioidomycosis infection is characterized by a Th1 response with secretion of IFN-c and basal levels of IL-10 and IL-4. In the acute form or juvenile a Th2 profile is predominant, with production of IL-10 and IL-4 and low levels of IFN-c. In chronic or adult, a mixed Th1 and Th2 profile is observed. The IFN-c can be induced by another cytokine, IL-12, and both are important in cellular immune response. In experimental models, IFN-c confers resistance to infection caused by Paracoccidioides brasiliensis and deficiency of IL-12p40 can cause susceptibility to this mycosis. The differential production of these cytokines may be associated with genetic polymorphisms. Objective: To investigate the association between the single nucleotide polymorphisms (SNP) IL-12B 3¢ UTR +1188 A/C and IFNG +874 T/A and the clinical forms of paracoccidioidomycosis. Materials and methods: DNA was obtained from peripheral blood samples from patients with paracoccidioidomycosis and healthy subjects. SNP IL-12B 3¢ UTR +1188 A/C was investigated with PCR-RFLP (PCR-Restriction Fragment Length Polymorphism) and SNP IFNG +874 T/A was investigated with PCR-ARMS (Polymerase Chain Reaction - Amplification Refractory Mutational System). Amplified products were visualized with electrophoresis on agarose gel. Results: In the SNP IFNG +874 T/A, the AA genotype was more frequent in the chronic multifocal and TA genotype in the unifocal. Considering the SNP IL-12B 3¢ UTR +1188 A/C there was a tendency for predominance of AC genotype in patients with chronic form, while the AA genotype was more frequent in acute. Despite this apparent difference among the groups, no statistically significant difference was observed in genotype distribution and alleles A, T and C. Conclusions: In this study there was no association between genetic polymorphisms of IL-12B 3¢ UTR +1188 A/C or IFNG +874 T/A and paracoccidioidomycosis, as well as with different clinical forms.conferenceObject Candida tropicalis bloodstream infection in two teaching hospitals in Sao Paulo State, Brazil(2012) MAGRI, M. M. C.; DUARTE, R. D.; FREITAS, V. L. T.; COSTA, S. F.; REIS, F. A.; MORETTI, M. L.; YASUDA, M. A. ShikanaiCandidemia is considered one of the most frequent causes of bloodstream infections around the world, causing considerable morbimortality and increasing the length of hospitalization, and overall cost of medical expenses. Aims: To compare molecular methods for characterization of Candida tropicalis isolates of two teaching hospitals; to study the antifungal resistance profile Materials and methods: In this study 61 Candida tropicalis isolates (43 patients) from several unities of Hospital das Clı ́nicas da Faculdade de Medicina da USP and Hospital das Clínicas da UNICAMP have been analyzed by antifungigram and molecular typing. Antifungigram was studied through two methods: CLSI and EUCAST. For molecular characterization, RAPD (Randomic Arbitrary Polymorphic DNA) and Pulsed Field Electrophoresis (PFGE) with restriction enzymes (SfiI, SmaI, BssHII e NaeI) were analyzed. Results: BssHII presented best performance for discrimination of the isolates. PFGE was able to show differences among multiple isolates of the same patient. ‘‘Trailing’’ was observed by CLSI and EUCAST in 30 – 70% of the samples tested with azoles. Resistance to fluconazole was observed in 5% of the isolates. Four patients presented with positive blood culture during antifungal therapy: only one was resistant to fluconazole (CLSI and EUCAST). Conclusions: No correlation was found between genetic variability and resistance profile to antifungal drugs. In this study, specific clones have not been observed in each teaching hospital.