EMMANUEL DIAS NETO
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LIM/27 - Laboratório de Neurociências, Hospital das Clínicas, Faculdade de Medicina
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- Da arte de fazer ciência e de comer jabuticabas – Uma homenagem ao Prof. Dr. Ricardo Renzo Brentani(2012) DIAS-NETO, Emmanuel; GATTAZ, Wagner E.
- Lithium activates brain phospholipase A2 and improves memory in rats: implications for Alzheimer's disease(2016) MURY, Fabio B.; SILVA, Weber C. da; BARBOSA, Nadia R.; MENDES, Camila T.; BONINI, Juliana S.; SARKIS, Jorge Eduardo Souza; CAMMAROTA, Martin; IZQUIERDO, Ivan; GATTAZ, Wagner F.; DIAS-NETO, EmmanuelPhospholipase A2 (Pla2) is required for memory retrieval, and its inhibition in the hippocampus has been reported to impair memory acquisition in rats. Moreover, cognitive decline and memory deficits showed to be reduced in animal models after lithium treatment, prompting us to evaluate possible links between Pla2, lithium and memory. Here, we evaluated the possible modulation of Pla2 activity by a long-term treatment of rats with low doses of lithium and its impact in memory. Wistar rats were trained for the inhibitory avoidance task, treated with lithium for 100 days and tested for perdurability of long-term memory. Hippocampal samples were used for quantifying the expression of 19 brain-expressed Pla2 genes and for evaluating the enzymatic activity of Pla2 using group-specific radio-enzymatic assays. Our data pointed to a significant perdurability of long-term memory, which correlated with increased transcriptional and enzymatic activities of certain members of the Pla2 family (iPla2 and sPla2) after the chronic lithium treatment. Our data suggest new possible targets of lithium, add more information on its pharmacological activity and reinforce the possible use of low doses of lithium for the treatment of neurodegenerative conditions such as the Alzheimer's disease.
- Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia (vol 43, pg 978, 2009)(2011) MARTINS-DE-SOUZA, Daniel; GATTAZ, Wagner F.; SCHMITT, Andrea; MACCARRONE, Giuseppina; HUNYADI-GULYAS, Eva; EBERLIN, Marcos N.; SOUZA, Gustavo H. M. F.; MARANGONI, Sergio; NOVELLO, Jose C.; TURCK, Christoph W.; DIAS-NETO, Emmanuel
conferenceObject PROTEOME ANALYSES OF POST-MORTEMBRAIN TISSUE FROM PATIENTS WITH SCHIZOPHRENIA SUGGEST DYSFUNCTION OF OLIGODENDROCYTES AND ASTROCYTES AND POTENTIAL BIOMARKER CANDIDATES?(2012) MARTINS-DE-SOUZA, Daniel; MACCARRONE, Giuseppina; SCHMITT, Andrea; FALKAI, Peter; DIAS-NETO, Emmanuel; GATTAZ, Wagner F.; TURCK, Chris W.- Conjugated linoleic acid-enriched butter improved memory and up-regulated phospholipase A(2) encoding-genes in rat brain tissue(2015) GAMA, Marco A. S.; RAPOSO, Nadia R. B.; MURY, Fabio B.; LOPES, Fernando C. F.; DIAS-NETO, Emmanuel; TALIB, Leda L.; GATTAZ, Wagner F.Reduced phospholipase A(2) (PLA(2)) activity has been reported in blood cells and in postmortem brains of patients with Alzheimer disease (AD), and there is evidence that conjugated linoleic acid (CLA) modulates the activity of PLA(2) groups in non-brain tissues. As CLA isomers were shown to be actively incorporated and metabolized in the brains of rats, we hypothesized that feeding a diet naturally enriched in CLA would affect the activity and expression of Pla(2)-encoding genes in rat brain tissue, with possible implications for memory. To test this hypothesis, Wistar rats were trained for the inhibitory avoidance task and fed a commercial diet (control) or experimental diets containing either low CLA- or CLA-enriched butter for 4 weeks. After this period, the rats were tested for memory retrieval and killed for tissue collection. Hippocampal expression of 19 Pla(2) genes was evaluated by qPCR, and activities of PLA(2) groups (cPLA(2), iPLA(2), and sPLA(2)) were determined by radioenzymatic assay. Rats fed the high CLA diet had increased hippocampal mRNA levels for specific PLA(2) isoforms (iPla(2)g6 gamma; cPla(2)g4a, sPla(2)g3, sPla(2)g1b, and sPla(2)g12a) and higher enzymatic activity of all PLA(2) groups as compared to those fed the control and the low CLA diet. The increment in PLA(2) activities correlated significantly with memory enhancement, as assessed by increased latency in the step-down inhibitory avoidance task after 4 weeks of treatment (r(s) = 0.69 for iPLA(2), P < 0.001; r(s) = 0.81 for cPLA(2), P < 0.001; and r(s) = 0.69 for sPLA(2), P < 0.001). In face of the previous reports showing reduced PLA(2) activity in AD brains, the present findings suggest that dairy products enriched in cis-9, trans-11 CLA may be useful in the treatment of this disease.