KATIA RAMOS MOREIRA LEITE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cirurgia, Faculdade de Medicina - Docente
LIM/55 - Laboratório de Urologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/55 - Laboratório de Urologia, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/26 - Laboratório de Pesquisa em Cirurgia Experimental, Hospital das Clínicas, Faculdade de Medicina
15 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 15
- Why do we keep reporting high-grade prostatic intraepithelial neoplasia (HGPIN)?(2016) LEITE, Katia R. M.
conferenceObject THE PHENOMENON OF EPITHELIAL-MESENCHYMAL TRANSITION IS ASSOCIATED WITH THE PROGRESSION OF PROSTATE CANCER(2016) PONTES JR., Jose; COELHO, Rafael; CORDEIRO, Mauricio; BETONI, Giuliano; OLIVEIRA, Luiz; REIS, Sabrina; LEITE, Katia; SROUGI, Miguel; NAHAS, William- Role of Genetic Polymorphisms in the Development and Prognosis of Sporadic and Familial Prostate Cancer(2016) REIS, Sabrina T.; VIANA, Nayara I.; LEITE, Katia R. M.; DIOGENES, Erico; ANTUNES, Alberto A.; ISCAIFE, Alexandre; NESRALLAH, Adriano J.; PASSEROTTI, Carlo C.; SROUGI, Victor; PONTES-JUNIOR, Jose; SALLES, Mary Ellen; NAHAS, William C.; SROUGI, MiguelBackgrounds Our aim was to evaluate the role of 20 genetic polymorphisms in the development and prognosis of sporadic and familial PC. A case-control study of 185 patients who underwent radical prostatectomy from 1997 to 2011. These patients were divided into two groups based on their family history. Gleason grade, PSA value and pathological TNM 2002 stage were used as prognostic factors. Blood samples from 70 men without PC were used as controls. The SNPs were genotyped using a TaqMan SNP Genotyping Assay Kit. Results Considering susceptibility, the polymorphic allele in the SNP rs2660753 on chromosome 3 was significantly more prevalent in controls (p = 0.01). For familial clustering, the polymorphic homozygote genotype of the SNP rs7931342 was five times more frequent in patients with familial PC compared to sporadic PC (p = 0.01). Regarding the SNP 1447295, the polymorphic homozygote genotype was more prevalent in patients with organ-confined PC (p = 0.05), and most importantly, the polymorphic allele occurred more frequently in patients without biochemical recurrence (p = 0.01). Kaplan-Meier analysis showed a median biochemical recurrence free survival of 124.2 compared to 85.6 months for patients with the wild-type allele (p = 0.007). Conclusion Our findings provide the evidence for the association of 20 recently highlighted SNPs and their susceptibility, familial clustering, staging, Gleason score and biochemical recurrence of PC. We believe that the association between these SNPs and PC may contribute to the development of alternative tools that can facilitate the early detection and prognosis of this disease.
conferenceObject DIAGNOSTIC ACCURACY OF URINARY beta 2-MICROGLOBULIN, CA19-9, NGAL AND KIM-1 IN URETEROPELVIC JUNCTION OBSTRUCTION IN ADULTS(2016) MIRANDA, Eduardo; BESSA JR., Jose de; LOPES, Roberto; SOUGI, Victor; ANDRADE, Hiury; BANDEIRA, Rodolfo; ARAP, Marco; MITTRE, Anuar; VIANA, Nayara; REIS, Sabrina dos; LEITE, Katia; SOUGI, Miguel; DUARTE, Ricardo- Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection(2016) ROSSI, Ana Flavia Teixeira; CADAMURO, Aline Cristina Targa; BISELLI-PERICO, Joice Matos; LEITE, Katia Ramos Moreira; SEVERINO, Fabio Eduardo; REIS, Patricia P.; CORDEIRO, Jose Antonio; SILVA, Ana ElizabeteHelicobacter pylori cause chronic inflammation favouring gastric carcinogenesis, and its eradication may prevent malignant transformation. We evaluated whether H. pylori infection and its eradication modify the expression of inflammatory mediators in patients with chronic gastritis. Furthermore, we assessed whether microRNAs modulate inflammatory pathways induced by H. pylori and identified miRNA-gene interaction networks. mRNA and protein expression of TNFA, IL6, IL1B, IL12A, IL2 and TGFBRII and miRNAs miR-103a-3p, miR-181c-5p, miR-370-3p, miR-375 and miR-223-3p were evaluated in tissue samples from 20 patients with chronic gastritis H. pylori negative (Hp-) and 31 H. pylori positive (Hp+), before and three months after bacterium eradication therapy, in comparison with a pool of Hp- normal gastric mucosa. Our results showed that H. pylori infection leads to up-regulation of TNFA, IL6, IL12A and IL2 and down-regulation of miRNAs. Bacterium eradication reduces the expression of TNFA and IL6 and up-regulates TGFBRII and all investigated miRNAs, except miR-223-3p. Moreover, transcriptional profiles of inflammatory mediators and miRNAs after eradication are different from the non-infected group. Deregulated miRNA-mRNA interaction networks were observed in the Hp+ group before and after eradication. Therefore, miRNAs modulated cytokine expression in the presence of H. pylori and after its eradication, suggesting that miRNAs participate in the pathological process triggered by H. pylori in the gastric mucosa.
conferenceObject GENETIC ALTERATIONS RELATED TO CYSTINURIA - A GENOME WIDE ASSOCIATION ANALYSIS(2016) REIS, Sabrina; VIANA, Nayara; LEITE, Katia; MAGIONI, Felipe; TORRICELLI, Fabio; MARCHINNI, Giovanni; SROUGI, Miguel; MAZZUCCHI, Eduardo- MMP9 overexpression is associated with good surgical outcome in children with UPJO: Preliminary results(2016) REIS, Sabrina Thalita; LEITE, Katia R. M.; VIANA, Nayara Izabel; LOPES, Roberto Iglesias; MOURA, Caio Martins; IVANOVIC, Renato F.; MACHADO, Marcos; DENES, Francisco Tibor; GIRON, Amilcar; NAHAS, William Carlos; SROUGI, Miguel; PASSEROTTI, Carlo C.Background: Ureteropelvic junction obstruction (UPJO) diagnosed prenatally occurs in 1: 150 -1: 1200 pregnancies. Although many studies investigating the molecular changes of this obstructed segment have been performed, the underlying mechanisms are still unclear. The role of extracellular matrix (ECM) components remains controversial, and the investigations in the field of ECM changes, might help the better understanding of the pathogenesis of this common condition. The aim of the present study was to investigate for the first time in the literature whether MMP9 and its specific inhibitors, TIMP1 and RECK, are expressed in a reproducible, specific pattern in UPJ. Methods: UPJO specimens were obtained from 16 children at the time of dismembered pyeloplasty due to intrinsic UPJ stenosis. Expression levels of the three genes (MMP9, TIMP1 and RECK) were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Then correlated the expression levels of the genes according to grade study population that was divided in 2 categories according to Society of Fetal Urology classification, grade 3 (moderate) and 4 (severe). For DTPA we subdivided the childrens in 2 groups, obstructive (T 1/2 more than 20 min) and partial obstructive (T 1/2 between 10 and 20 min) and success in a surgery was defined as decrease in T 1/ 2 to less than 20 min, absence of symptoms, improving renal function and decreasing dilatation on successive exams. Results: MMP9 was underexpressed and TIMP1 and RECK were overexpressed in children with obstructive DTPA but the differences were not statistically significant. Overexpression of MMP9 was higher among patients with severe grade of UPJ compared to those with moderate grade. Surprisingly expression levels of MMP-9 was three times higher in children who were successfully treated by surgery (n = 10) (p = 0.072), so those who were followed for at least 1 year after surgery and remained with improvement in renal function and decreasing dilation on intravenous urogram and TIMP-1 was underexpressed in 100 % of this cases (p = 0.00). Conclusions: We showed an increase in expression of MMP9 and a decrease in expression of TIMP1 in children who improving renal function and decreasing dilation after surgery. We believe that the higher expression of MMP9 in these cases can reflect an increase in degradation and remodeling process that could be used as a marker for surgical outcome.
- International Society of Urological Pathology (ISUP) Grading of Prostate Cancer(2016) EGEVAD, Lars; DELAHUNT, Brett; EVANS, Andrew J.; GRIGNON, David J.; KENCH, James G.; KRISTIANSEN, Glen; LEITE, Katia R.; SAMARATUNGA, Hemamali; SRIGLEY, John R.
conferenceObject GSTM1, GSTT1, GSTP1 Polymorphisms, Quality of Life, Habits, Environmental Exposure and Predisposition to Bladder Cancer(2016) PALOPPI, Isis; PIMENTA, Ruan C. A.; REIS, Sabrina T.; NAHAS, Willian C.; SROUGI, Miguel; CURY, Jose; SAIKI, Mitiko; LEITE, Katia R. M.conferenceObject GSTM1, GSTT1, GSTP1 Polymorphisms, Quality of Life, Habits, Environmental Exposure and Predisposition to Bladder Cancer(2016) PALOPPI, Isis; PIMENTA, Ruan C. A.; REIS, Sabrina T.; NAHAS, Willian C.; SROUGI, Miguel; CURY, Jose; SAIKI, Mitiko; LEITE, Katia R. M.