NATHALIA MONTOURO PINHEIRO MENEGASSO
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- Alpha-7 nicotinic receptor stimulation reduces airway inflammation in a murine model of asthma(2016) SANTANA, Fernanda Paula Roncon; MIRANDA, Claudia Jeane Claudino de Pontes; PINHEIRO, Nathalia Montouro; PERINI, Adenir; CAPERUTO, Luciana Chagas; TIBERIO, Iolanda de Fatima Lopes Calvo; PRADO, Marco Antonio Maximo; MARTINS, Milton de Arruda; PRADO, Vania Ferreira; PRADO, Carla Maximo
- Effects of VAChT reduction on the time course of lung inflammation in mice with acute lung injury(2016) PINHEIRO, Nathalia; SANTANA, Fernanda; CAPERUTO, Luciana; TIBERIO, Iolanda; PRADO, Vania; PRADO, Marco Antonio; MARTINS, Milton; PRADO, Carla
- The role of monoterpenes derived from essential oils in lung alteration in a model of emphysema(2016) PEREIRA, Ellen; GUERREIRO, Marina; SANTANA, Fernanda; PINHEIRO, Nathalia; CAPELLO, Tabata; LOPES, Fernanda; OLIVO, Clarice; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, Carla
- Evidences of Herbal Medicine-Derived Natural Products Effects in Inflammatory Lung Diseases(2016) SANTANA, Fernanda Paula R.; PINHEIRO, Nathalia M.; MERNAK, Marcia Isabel B.; RIGHETTI, Renato F.; MARTINS, Milton A.; LAGO, Joao H. G.; LOPES, Fernanda D. T. Q. dos Santos; TIBERIO, Iolanda F. L. C.; PRADO, Carla M.Pulmonary inflammation is a hallmark of many respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and acute respiratory syndrome distress (ARDS). Most of these diseases are treated with anti-inflammatory therapy in order to prevent or to reduce the pulmonary inflammation. Herbal medicine-derived natural products have been used in folk medicine and scientific studies to evaluate the value of these compounds have grown in recent years. Many substances derived from plants have the biological effects in vitro and in vivo, such as flavonoids, alkaloids, and terpenoids. Among the biological activities of natural products derived from plants can be pointed out the anti-inflammatory, antiviral, antiplatelet, antitumor anti-allergic activities, and antioxidant. Although many reports have evaluated the effects of these compounds in experimental models, studies evaluating clinical trials are scarce in the literature. This review aims to emphasize the effects of these different natural products in pulmonary diseases in experimental models and in humans and pointing out some possible mechanisms of action.
- Structurally Related Monoterpenes p-Cymene, Carvacrol and Thymol Isolated from Essential Oil from Leaves of Lippia sidoides Cham. (Verbenaceae) Protect Mice against Elastase-Induced Emphysema(2016) GAMES, Ellen; GUERREIRO, Marina; SANTANA, Fernanda R.; PINHEIRO, Nathalia M.; OLIVEIRA, Emerson A. de; LOPES, Fernanda D. T. Q. S.; OLIVO, Clarice R.; TIBERIO, Iolanda F. L. C.; MARTINS, Milton A.; LAGO, Joao Henrique G.; PRADO, Carla M.Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and inflammation. Natural products, such as monoterpenes, displayed anti-inflammatory and anti-oxidant activities and can be used as a source of new compounds to COPD treatment. Our aim was to evaluate, in an elastase-induced pulmonary emphysema in mice, the effects of and underlying mechanisms of three related natural monoterpenes (p-cymene, carvacrol and thymol) isolated from essential oil from leaves Lippia sidoides Cham. (Verbenaceae). Methods: Mices received porcine pancreatic elastase (PPE) and were treated with p-cymene, carvacrol, thymol or vehicle 30 min later and again on 7th, 14th and 28th days. Lung inflammatory profile and histological sections were evaluated. Results: In the elastase-instilled animals, the tested monoterpenes reduced alveolar enlargement, macrophages and the levels of IL-1 beta, IL-6, IL-8 and IL-17 in bronchoalveolar lavage fluid (BALF), and collagen fibers, MMP-9 and p-65-NF-kappa B-positive cells in lung parenchyma (p < 0.05). All treatments attenuated levels of 8-iso-PGF2 alpha but only thymol was able to reduced exhaled nitric oxide (p < 0.05). Conclusion: Monoterpenes p-cymene, carvacrol and thymol reduced lung emphysema and inflammation in mice. No significant differences among the three monoterpenes treatments were found, suggesting that the presence of hydroxyl group in the molecular structure of thymol and carvacrol do not play a central role in the anti-inflammatory effects.
- Low level laser therapy reduces acute lung inflammation without impairing lung function(2016) CURY, Vivian; LIMA, Thais Martins de; PRADO, Carla Maximo; PINHEIRO, Nathalia; ARIGA, Suely K. K.; BARBEIRO, Denise F.; MORETTI, Ana I.; SOUZA, Heraldo P.Acute lung injury is a condition characterized by exacerbate inflammatory reaction in distal airways and lung dysfunction. Here we investigate the treatment of acute lung injury (ALI) by low level laser therapy (LLLT), an effective therapy used for the treatment of patients with inflammatory disorders or traumatic injuries, due to its ability to reduce inflammation and promote tissue regeneration. However, studies in internal viscera remains unclear. C57BL/6 mice were treated with intratracheal lipopolysaccharide (LPS) (5 mg/kg) or phosphate buffer saline (PBS). Six hours after instillation, two groups were irradiated with laser at 660 nm and radiant exposure of 10 J/cm(2). Intratracheal LPS inoculation induced a marked increase in the number of inflammatory cells in perivascular and alveolar spaces. There was also an increase in the expression and secretion of cytokines (TNF-alpha, IL-1 beta, IL-6,) and chemokine (MCP-1). The LLLT application induced a significant decrease in both inflammatory cells influx [GRAPHICS] and inflammatory mediators secretion. These effects did not affect lung mechanical properties, since no change was observed in tissue resistance or elastance. In conclusion LLLT is able to reduce inflammatory reaction in lungs exposed to LPS without affecting the pulmonary function and recovery.
- Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation(2016) SAKODA, Camila Pivari Pedroso; TOLEDO, Alessandra Choqueta de; PERINI, Adenir; PINHEIRO, Nathalia Montouro; HIYANE, Meire Ioshie; GRECCO, Simone dos Santos; TIBERIO, Iolanda de Fatima Lopes Calvo; CAMARA, Niels Olsen Saraiva; MARTINS, Milton de Arruda; LAGO, Joao Henrique Ghilardi; RIGHETTI, Renato Fraga; PRADO, Carla MaximoBackground and purpose: Asthma is a disease of high prevalence and morbidity that generates high costs in hospitalization and treatment. Although the airway is involved in the physiopathology of asthma, there is also evidence of the importance of vascular and lung parenchyma inflammation and remodeling, which can contribute to the functional pulmonary alterations observed in asthmatic patients. Our aim was to evaluate treatment using sakuranetin, a flavone isolated from the twigs of Baccharis retusa (Asteraceae), on vascular and lung parenchyma alterations in an experimental murine model of asthma. Methods: Male BALB/c mice were subjected to a sensitization protocol with ovalbumin for 30 days and were treated with or without sakuranetin (20 mg/kg/mice) or dexamethasone (5 mg/kg/mice); then, the lungs were collected for histopathological analysis. We evaluated extracellular matrix remodeling (collagen and elastic fibers), inflammation (eosinophils and NF-kB) and oxidative stress (8-isoprostane) in the pulmonary vessels and lung parenchyma. The thickness of the vascular wall was quantified, as well as the vascular endothelial growth factor (VEGF) levels. Results: We demonstrated that sakuranetin reduced the number of eosinophils and elastic fibers in both the pulmonary vessels and the lung parenchyma, probably due to a reduction of oxidative stress and of the transcription factor NF-kB and VEGF levels in the lung. In addition, it reduced the thickness of the pulmonary vascular wall. The treatment had no effect on the collagen fibers. In most of the parameters, the effect of sakuranetin was similar to the dexamethasone effect. Conclusions and implications: Sakuranetin had anti-inflammatory and antioxidant effects, preventing vascular and distal parenchyma changes in this experimental model of asthma.
- Sakuranetin treatment from baccharis retusa reduces acid mucus in a murine model of asthma(2016) SANTANA, Fernanda Paula Roncon; RAMOS, Thais Lourenco; TAVARES, Anna Carolina Morais; PINHEIROS, Nathalia Montouro; SADOKA, Camila Pivari Pedroso; GRECCO, Simone Dos Santos; MACCHIONE, Mariangela; YOSHIZAKI, Kelly; TIBERIO, Iolanda De Fatima Lopes Calvo; MARTINS, Milton de Arruda; LAGO, Joao Henrique Ghilardi; TOLEDO, Alessandra Choqueta; PRADO, Carla Maximo