MARIA IRMA SEIXAS DUARTE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses, Hospital das Clínicas, Faculdade de Medicina - Líder
5 resultados
Resultados de Busca
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- Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock(2019) DUARTE-NETO, Amaro Nunes; CRODA, Julio; PAGLIARI, Carla; SORIANO, Francisco Garcia; NICODEMO, Antonio Carlos; DUARTE, Maria Irma SeixasObjectives: To compare microscopic and immunologic features in the spleens of patients who died of pulmonary hemorrhage and shock caused by leptospirosis (11 cases) or Gram-positive/-negative bacterial septic shock (10 cases) to those from control spleens (12 cases from splenectomy). Methodology: Histological features in the red pulp and white pulp were analyzed using archived samples by a semi quantitative score. Immunohistochemistry was used for the recognition of immune cell markers, cytokines, caspase-3 and Leptospira antigens. Results: The control group differed significantly from the leptospirosis and septic shock patients which demonstrate strong similarities: diffuse congestion in the red pulp with a moderate to intense infiltration of plasma cells and polymorphonuclear cells; follicles with marked atrophy; high density of CD20(+) cells; low density of NK, TCD4(+) and active caspase-3 positive cells and strong expression of IL-10; leptospirosis patients had higher S100 and TNF-alpha positive cells in the spleen than the other groups. Conclusion: The results suggest that an immunosuppressive state develops at the terminal stage of severe leptospirosis with pulmonary hemorrhage and shock similar to that of patients with septic shock, with diffuse endothelial activation in the spleen, splenitis, and signs of disturbance in the innate and adaptive immunity in the spleen. The presence of leptospiral antigens in 73% of the spleens of the leptospirosis patients suggests the etiological agent contributes directly to the pathogenesis of the lesions. Our results support therapeutic approaches involving antibiotic and immunomodulatory treatments for leptospirosis patients and suggest that leptospirosis patients, which are usually young men with no co-morbidities, form a good group for studying sepsis and septic shock.
- Yellow fever and orthotopic liver transplantation: new insights from the autopsy room for an old but re-emerging disease(2019) DUARTE-NETO, Amaro N.; CUNHA, Marielton dos P.; MARCILIO, Izabel; SONG, Alice T. W.; MARTINO, Rodrigo B. de; HO, Yeh-Li; POUR, Shahab Z.; DOLHNIKOFF, Marisa; SALDIVA, Paulo H. N.; DUARTE, Maria I. S.; TAKAKURA, Cleusa F.; LIMA, Fabiana R.; TANIGAWA, Ryan Y.; IGLEZIAS, Silvia D'A; KANAMURA, Cristina T.; SANTOS, Angela B. G. dos; PERONDI, Beatriz; ZANOTTO, Paolo M. de A.; D'ALBUQUERQUE, Luiz A. C.; ALVES, Venancio A. F.Aims The clinical spectrum of yellow fever (YF) ranges from asymptomatic to fulminant hepatitis. During the sylvatic YF epidemic in the metropolitan area of Sao Paulo, Brazil in 2018, seven orthotopic liver transplantations (OLTs) were performed in our institution to treat fulminant YF hepatitis. Three patients recovered, while four patients died following OLT. The autopsy findings of all these cases are presented herein as the first description of YF in transplanted patients. Methods and results All patients were men, aged 16-40 years, without vaccination to YF virus (YFV). All organs were examined, with tissue sampling for histopathological analysis. Detection of YF virus antigens (YFV Ag) was performed with two primary antibodies (mouse polyclonal anti-YFV antibody directed to wild strain and a goat anti-YF virus antibody), and RT-PCR assays were utilised to detect YFV-RNA. All the cases depicted typical findings of YF hepatitis in the engrafted liver. The main extrahepatic findings were cerebral oedema, pulmonary haemorrhage, pneumonia, acute tubular necrosis and ischaemic/reperfusion pancreatitis. Of the four cases, the YVF Ag was detected in the heart in one case, liver and testis in three cases, and the kidney and spleen in all four cases. All four cases had YF virus RNA detected by RT-PCR in the liver and in other organs. Conclusions Infection of the engrafted liver and other organs by YFV, possibly combined with major ischaemic systemic lesions, may have led to the death of four of the seven patients undergoing OLT.
- Human immunodeficiency virus infection of the liver(2018) DUARTE, M. I. Seixas; DUARTE NETO, A. N.
bookPart Doenças tropicais: Leishmaniose visceral(2022) DUARTE, Maria Irma Seixas; DUARTE NETO, Amaro NunesbookPart Infecção pelo HIV e AIDS(2022) MILITO, Cristiane Bedran; MORAIS, José Carlos; BARROSO, Paulo Feijó; CHIMELLI, Leila; DUARTE, Maria Irma Seixas; CUZZI, Tullia; PANNAIN, Vera Lucia Nunes; PITTELLA, José Eymard Homem; DUARTE NETO, Amaro Nunes