ALYNE FRANCA MARQUES
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Methotrexate associated to lipid core nanoparticles improves cardiac allograft vasculopathy and the inflammatory profile in a rabbit heart graft model(2017) FIORELLI, A. I.; LOURENCO-FILHO, D. D.; TAVARES, E. R.; CARVALHO, P. O.; MARQUES, A. F.; GUTIERREZ, P. S.; MARANHAO, R. C.; STOLF, N. A. G.Coronary allograft vasculopathy is an inflammatory-proliferative process that compromises the long-term success of heart transplantation and has no effective treatment. A lipid nanoemulsion (LDE) can carry chemotherapeutic agents in the circulation and concentrates them in the heart graft. The aim of the study was to investigate the effects of methotrexate (MTX) associated to LDE. Rabbits fed a 0.5% cholesterol diet and submitted to heterotopic heart transplantation were treated with cyclosporine A (10 mg.kg(-1).day(-1) orally) and allocated to treatment with intravenous LDE-MTX (4 mg/kg, weekly, n=10) or with weekly intravenous saline solution (control group, n=10), beginning on the day of surgery. Animals were euthanized 6 weeks later. Compared to controls, grafts of LDE-MTX treated rabbits showed 20% reduction of coronary stenosis, with a four-fold increase in vessel lumen and 80% reduction of macrophage staining in grafts. Necrosis was attenuated by LDE-MTX. Native hearts of both LDE-MTX and Control groups were apparently normal. Gene expression of lipoprotein receptors was significantly greater in grafts compared to native hearts. In LDE-MTX group, gene expression of the pro-inflammatory factors tumor necrosis factor-alpha, monocyte chemoattractant protein-1, interleukin-18, vascular cell adhesion molecule-1, and matrix metalloproteinase-12 was strongly diminished whereas expression of anti-inflammatory interleukin-10 increased. LDE-MTX promoted improvement of the cardiac allograft vasculopathy and diminished inflammation in heart grafts.
conferenceObject Effects of treatment with methotrexate associated to lipid nanoparticles on diabetic cardiomyopathy in rats(2016) MARQUES, A. F.; GUIDO, M. C.; TAVARES, E. R.; BISPO, D. L.; MELLO, M. D.; SALEMI, V. M.; MARANHAO, R. C.conferenceObject Methotrexate carried in lipid nanoparticles pronouncedly improves the functional and inflammatory status of the heart following acute myocardium infarction induced in rats(2016) GUIDO, M. C.; MARQUES, A. F.; TAVARES, E. R.; BISPO, D. L.; LIMA, A. D.; MELO, M. D.; KALIL-FILHO, R.; NICOLAU, J. C.; SALEMI, V. M.; MARANHAO, R. C.conferenceObject Methotrexate reduces TNF-alpha and beta adrenergic receptor expression and improves left ventricle function in induced Takotsubo myocardiopathy in rats(2016) BISPO, D. L.; GUIDO, M. C.; MARQUES, A. F.; TAVARES, E. R.; MELO, M. D.; SALEMI, V. M.; MARANHAO, R. C.