VANESSA DOS SANTOS MORAIS
Projetos de Pesquisa
Unidades Organizacionais
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
14 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 14
- Multiple clades of Husavirus in South America revealed by next generation sequencing(2021) RAMOS, Endrya do Socorro Foro; ROSA, Ulisses Alves; RIBEIRO, Geovani de Oliveira; VILLANOVA, Fabiola; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; MORAIS, Vanessa dos Santos; ARAUJO, Emerson Luiz Lima; PANDEY, Ramendra Pati; RAJ, V. Samuel; SABINO, Ester Cerdeira; DENG, Xutao; DELWART, Eric; LUCHS, Adriana; LEAL, Elcio; COSTA, Antonio Charlys daHusavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.
- Aedes aegypti Totivirus identified in mosquitoes in the Brazilian Amazon region(2023) LEAL, Elcio; RIBEIRO, Edcelha Soares D'Athaide; MONTEIRO, Fred Julio Costa; MARQUES, Julia Pantoja; MENDES, Diuliana dos Santos; MORAIS, Vanessa S.; ARAUJO, Emerson Luiz Lima; PANDEY, Ramendra Pati; CHANG, Chung-Ming; DENG, Xutao; DELWART, Eric; COSTA, Antonio Charlys da; LIMA, KledoaldoThe totiviridae family contains viruses with double-stranded RNA genomes of 4.6-7.0 kpb, which encode a capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), and they are approximately 40 nm in diameter with icosahedral symmetry. Totiviruses were first isolated from mosquitoes collected in Shaanxi Province (China). Here, we report a new Aedes aegypti Totivirus (AaTV) identified in mosquitoes from the Amazon rainforest. Mosquitoes (Diptera: Culicidae) were collected from a forest reserve belonging to the Amazon forest in the city of Macapa, Amapa state, Northern Brazil. A viral sequence with a 5748 nucleotide length that was nearly identical to Aedes aegypti Totivirus (AaTV), here named Aedes aegypti Totivirus BR59AP, was detected. A detailed molecular analysis was performed and shows that AaTV-BR59AP is highly related to the AaTV strain from the Caribbean region. We emphasize the importance of the characterization of new viruses in mosquitoes to deepen our understanding of viral diversity in insects and their potential role in disease.
- Aedes aegyptifrom Amazon Basin Harbor High Diversity of Novel Viral Species(2020) RIBEIRO, Geovani de Oliveira; MORAIS, Vanessa S.; MONTEIRO, Fred Julio Costa; RIBEIRO, Edcelha Soares D'Athaide; REGO, Marlisson Octavio da S.; SOUTO, Raimundo Nonato Picanco; VILLANOVA, Fabiola; TAHMASEBI, Roozbeh; HEFFORD, Philip Michael; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; FERNANDES, Licia Natal; COSTA, Antonio Charlys da; LEAL, ElcioViruses are the most diverse and abundant microorganisms on earth, highly adaptive to a wide range of hosts. Viral diversity within invertebrate hosts has gained notoriety in recent years in public health as several such viruses have been of medical importance.Aedes aegyptiserves as a vector for several viruses that have caused epidemics within the last year throughout Brazil; including Dengue, Zika and Chikungunya. This study aimed to identify new viral agents withinAedes aegyptimosquito in a city of the Amazonian region, where it is highly endemic. Metagenomic investigation was performed on 60 mosquito pools and viral RNA sequences present in their microbiota were characterized using genomic and phylogenetic tools. In total, we identified five putative novel virus species related to theSobemovirusgenus,Iflavirusgenus andPermutatetraviridaefamily. These findings indicate a diverse taxonomy of viruses present in the mosquito microbiota of the Amazon, the region with the greatest invertebrate diversity in the world.
- Metagenomic of Liver Tissue Identified at Least Two Genera of Totivirus-like Viruses in Molossus molossus Bats(2024) COUTO, Roseane da Silva; RAMOS, Endrya do Socorro Foro; ABREU, Wandercleyson Uchoa; RODRIGUES, Luis Reginaldo Ribeiro; MARINHO, Luis Fernando; MORAIS, Vanessa dos Santos; VILLANOVA, Fabiola; PANDEY, Ramendra Pati; DENG, Xutao; DELWART, Eric; COSTA, Antonio Charlys da; LEAL, ElcioThe Totiviridae family of viruses has a unique genome consisting of double-stranded RNA with two open reading frames that encode the capsid protein (Cap) and the RNA-dependent RNA polymerase (RdRpol). Most virions in this family are isometric in shape, approximately 40 nm in diameter, and lack an envelope. There are five genera within this family, including Totivirus, Victorivirus, Giardiavirus, Leishmaniavirus, and Trichomonasvirus. While Totivirus and Victorivirus primarily infect fungi, Giardiavirus, Leishmaniavirus, and Trichomonasvirus infect diverse hosts, including protists, insects, and vertebrates. Recently, new totivirus-like species have been discovered in fish and plant hosts, and through metagenomic analysis, a novel totivirus-like virus (named Tianjin totivirus) has been isolated from bat guano. Interestingly, Tianjin totivirus causes cytopathic effects in insect cells but cannot grow in mammalian cells, suggesting that it infects insects consumed by insectivorous bats. In this study, we used next-generation sequencing and identified totivirus-like viruses in liver tissue from Molossus molossus bats in the Amazon region of Brazil. Comparative phylogenetic analysis based on the RNA-dependent RNA polymerase region revealed that the viruses identified in Molossus bats belong to two distinct phylogenetic clades, possibly comprising different genera within the Totiviridae family. Notably, the mean similarity between the Tianjin totivirus and the totiviruses identified in Molossus bats is less than 18%. These findings suggest that the diversity of totiviruses in bats is more extensive than previously recognized and highlight the potential for bats to serve as reservoirs for novel toti-like viruses.
- Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics(2020) TAHMASEBI, Roozbeh; LUCHS, Adriana; TARDY, Kaelan; HEFFORD, Philip Michael; TINKER, Rory J.; EILAMI, Owrang; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; MORAIS, Vanessa dos Santos; MOREIRA, Carlos Henrique Valente; BUCCHERI, Renata; ARAUJO, Emerson Luiz Lima; VILLANOVA, Fabiola; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, Elcio; COSTA, Antonio Charlys daHuman enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8% (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9% (55/251) of samples and mixed infections in 37.4% (94/251), with RVA/HAdV-F being the most frequent association (21.5%; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.
- Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016(2021) LUCHS, Adriana; TARDY, Kaelan; TAHMASEBI, Roozbeh; MORILLO, Simone Guadagnucci; MILAGRES, Flavio Augusto de Padua; MORAIS, Vanessa dos Santos; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; AZEVEDO, Lais Sampaio de; SOUZA, Ellen Viana de; MEDEIROS, Roberta Salzone; SOUZA, Yasmin Franca Viana Pires de; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; LEAL, Elcio; COSTA, Antonio Charlys daThis study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages.
- Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016)(2020) SILVA-SALES, Marcelle; LEAL, Elcio; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; MORAIS, Vanessa dos Santos; MARCATTI, Roberta; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; DENG, Xutao; SABINO, Ester Cerdeira; DELWART, Eric; LUCHS, Adriana; COSTA, Antonio Charlys daSurveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination followup (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%). as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G21 3 [4] (5.6%) and G8P[41 (2.8%) strains, including the mixed G2/G81 3 141 infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-11E, G8-I and G12-111, and P[4]-V and P[8]-Ill of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area.
- Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil(2021) TINKER, Rory J.; COSTA, Antonio Charlys da; TAHMASEBI, Roozbeh; MILAGRES, Flavio Augusto de Padua; MORAIS, Vanessa dos Santos; PANDEY, Ramendra Pati; JOSE-ABREGO, Alexis; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; CUNHA, Mariana Sequetin; ARAUJO, Emerson Luiz Lima; GOMEZ, Mariela Martinez; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; LEAL, Elcio; LUCHS, AdrianaFrom 2010-2016, a total of 251 stool samples were screened for norovirus using next-generation sequencing (NGS) followed by phylogenetic analysis to investigate the genotypic diversity of noroviruses in rural and low-income urban areas in northern Brazil. Norovirus infection was detected in 19.9% (50/251) of the samples. Eight different genotypes were identified: GII.4_Sydney[P31] (64%, 32/50), GII.6[P7] (14%, 7/50), GII.17[P17] (6%, 3/50), GII.1[P33] (6%, 3/50), GII.3[P16] (4%, 2/50), GII.2[P16] (2%, 1/50), GII.2[P2] (2%, 1/50), and GII.4_New Orleans[P4] (2%, 1/50). Distinct GII.6[P7] variants were recognized, indicating the presence of different co-circulating strains. Elucidating norovirus genetic diversity will improve our understanding of their potential health burden, in particular for the GII.4_Sydney[P31] variant.
- Guapiacu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil(2021) RIBEIRO, Geovani de Oliveira; COSTA, Antonio Charlys da; GILL, Danielle Elise; RIBEIRO, Edcelha Soares D'Athaide; REGO, Marlisson Octavio da S.; MONTEIRO, Fred Julio Costa; VILLANOVA, Fabiola; NOGUEIRA, Juliana Silva; MAEDA, Adriana Yurika; SOUZA, Renato Pereira de; TAHMASEBI, Roozbeh; MORAIS, Vanessa S.; PANDEY, Ramendra Pati; RAJ, V. Samuel; SCANDAR, Sirle Abdo Salloum; VASAMI, Fernanda Gisele da Silva; D'AGOSTINO, Leandro Guaraglia; MAIORKA, Paulo Cesar; DENG, Xutao; NOGUEIRA, Mauricio Lacerda; SABINO, Ester Cerdeira; DELWART, Eric; LEAL, Elcio; CUNHA, Mariana SequetinClassical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative dISFV, termed Guapiacu virus (GUAPV). Distinct strains of GUAPV were isolated from pools of Aedes terrens and Aedes scapularis mosquitoes. Additionally, we also detected viral GUAPV RNA in a plasma sample of an individual febrile from the Amazon region (North of Brazil). Although GUAPV did not replicate in tested mammalian cells, 3 ' UTR secondary structures duplication and codon usage index were similar to pathogenic flavivirus.
- High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis(2021) RAMOS, Endrya do Socorro Foro; ROSA, Ulisses Alves; RIBEIRO, Geovani de Oliveira; VILLANOVA, Fabiola; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; MORAIS, Vanessa dos Santos; BERTANHE, Mayara; MARCATTI, Roberta; ARAUJO, Emerson Luiz Lima; WITKIN, Steven S.; DELWART, Eric; LUCHS, Adriana; COSTA, Antonio Charlys da; LEAL, ElcioEchoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.