RICARDO APARECIDO BAPTISTA NUCCI
Projetos de Pesquisa
Unidades Organizacionais
LIM/66, Hospital das Clínicas, Faculdade de Medicina
5 resultados
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- Ultrastructural effects of diabetes in the right atrium cardiomyocytes of elderly Wistar rats(2020) ANDRADE, Natalie Souza de; CHUCATA, Kemily Loren Barros; MAGALHAES, Walkyria Villegas; NUCCI, Ricardo Aparecido Baptista; COSTA-SANTOS, Nicolas Da; DIAS, Igor Roberto; LIMA, Hunter Douglas de Souza; MAIFRINO, Laura Beatriz Mesiano; SOUZA, Romeu Rodrigues deThe present study aimed to evaluate the effects of diabetes on quantitative parameters of right atrial cardiomyocytes of elderly rats. Wistar rats (14 months of age) were divided into two groups: streptozotocin-diabetic rats (DG) and control rats (CG). The groups were sacrificed at 16 months. Ultrafine sections of the right atrium were analyzed by electron microscopy. In elderly diabetic animals, histograms of the frequency distribution of natriuretic peptides according to their size showed increased number of small and medium peptides in relation to large peptides, which increased its numerical density leading to a decrease in the mean diameter of both natriuretic peptides. However, elderly diabetic animals remained normotensive. No significant difference was observed between the groups for the volume density of mitochondria, endoplasmic reticulum, and Golgi apparatus. In conclusion, elderly diabetic rats showed increased functional activity of atrial cardiomyocytes with greater production of natriuretic peptides in association with a quantitative maintenance of cytoplasmic components.
- Age-Related Effects on Right Femoral Bone of Male Wistar Rats: A Morphometric and Biomechanical Study(2022) PUELKER, Sheila Martins; CASTRO, Sonia Regina Ribeiro de; SOUZA, Romeu Rodrigues de; MAIFRINO, Laura Beatriz Mesiano; NUCCI, Ricardo Aparecido Baptista; SITTA, Maria do CarmoIntroduction Study of the variations of bone characteristics with age in different animal models is important to design musculoskeletal studies. Thus, this study aimed to evaluate the bone mass, dimensions, and biomechanical parameters of the femur in young, middle-aged, and aged Wistar rats. Materials and Methods Thirty male rats ( Rattus norvegicus ) were divided in three groups ( n = 10 per group)-3-month-old young rats, 12-month-old middle-aged rats, and 18-months-old aged rats. The right femurs were subjected sequentially to morphometric study (bone weight, cortical thickness) and biomechanical tests (maximum resistance strength and bone stiffness). Results We observed a significant increase in femur histological (cortical thickness) and biomechanical (maximum strength and bone stiffness) parameters with aging when compared with young animals. Conclusions With the advancing age, the right femoral bone of middle-aged and old animals had greater variations when compared with young animals. However, further studies with the aid of a comparison between right and left femur and other long bones in both male and female rats are needed to corroborate with our findings.
- Diaphragm muscle structure in the elderly: Findings from an autopsy study(2020) NUCCI, Ricardo Aparecido Baptista; SOUZA, Romeu Rodrigues de; SUEMOTO, Claudia Kimie; BUSSE, Alexandre Leopold; MAIFRINO, Laura Beatriz Mesiano; ANARUMA, Carlos Alberto; PASQUALUCCI, Carlos Augusto; JACOB-FILHO, WilsonSarcopenia is characterized as aging-related weakness and atrophy, which decreases the diaphragm force generating capacity leading to a decrease of expulsive non-ventilatory motor behaviors, which are critical for airway clearance. Thus, this study aimed to analyze the histopathology of the diaphragm in postmortem samples. Thirty individuals were included. Diaphragm samples were stained with hematoxylin and eosin for histopathological analysis. Picrosirius stain was used to highlight the collagen fibers. We observed a positive association between advancing age and histopathological findings in the diaphragm structure. We suggest that age is a key factor in increasing diaphragm muscle histopathology. However, further clinicopathological studies are needed to confirm our findings.
- Smoking Intensity Increases Diaphragm Muscle Injury: A Clinicopathologic Study(2023) NUCCI, Ricardo Aparecido Baptista; BUSSE, Alexandre Leopold; SOUZA, Romeu Rodrigues de; MAIFRINO, Laura Beatriz Mesiano; PASQUALUCCI, Carlos Augusto; ANARUMA, Carlos Alberto; LEITE, Renata Elaine Paraizo; RODRIGUEZ, Roberta Diehl; SUEMOTO, Claudia Kimie; JACOB-FILHO, WilsonBackground: Studying the effects of smoking intensity is important to evaluate the risk of tobacco use on a range of illnesses, such as as sarcopenia among the elderly. Thus, this study aimed to analyze the effects of pack-years of cigarette smoking on the diaphragm muscle (DIAm) histopathology of postmortem samples. Methods: Subjects were divided into three groups: never-smoker (n = 46); less than 30 pack-years of smoking (n = 12); and more than 30 pack-years of smoking (n = 30). Diaphragm samples were stained with Picrosirius red and hematoxylin and eosin stain for general structure. Results: Participants with more than 30 pack-years of cigarette smoking had a significant increase in adipocytes, blood vessels and collagen deposit, as well as an increase in histopathological alterations. Conclusions: Pack-years of smoking was associated with DIAm injury. However, further clinicopathological studies are needed to confirm our findings.
- Color Deconvolution as a Simple and Rapid Tool in Quantitative Biomedical Research(2024) NUCCI, Ricardo Aparecido Baptista; JACOB-FILHO, Wilson; SOUZA, Romeu Rodrigues de; MAIFRINO, Laura Beatriz Mesiano