RICARDO APARECIDO BAPTISTA NUCCI
Projetos de Pesquisa
Unidades Organizacionais
LIM/66, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Remodeling of the soleus muscle of ovariectomized old female rats submitted to resistance training and different diet intake(2020) BRAGGION, Glaucia Figueiredo; ORNELAS, Elisabete de Marco; CURY, Jurema Carmona Sattin; SOUSA, Jessica Pedroso de; NUCCI, Ricardo Aparecido Baptista; FONSECA, Fernando Luiz Affonso; MAIFRINO, Laura Beatriz MesianoSarcopenia is a common condition that is associated mainly with hormonal factors, nutritional status, physical activity, leading to a lower quality of life. Thus, this study aimed to evaluate the effects of diets with vegetable or animal proteins (AP) associated with resistance training on the structure of the soleus muscle in aged Wistar rats. The histochemical technique was used for the typing of muscle fibers, the cross-sectional area of myocytes, and volume densities of myocytes and interstitium. Picrosirius stain was used to quantify the collagen density. Diet intake, mainly animal protein, associated with resistance training leaded to muscle remodeling, and increased deposit of collagen fibers. We observed hypertrophy in animal groups that consumed animal protein diet, even the sedentary group, although more evident in those trained.
- Effects of resistance training on kidney morphology of aged ovariectomized rats(2020) MARCELINO, Marcelo Cristiano da Silva; MAGALHAES, Walkyria Villegas; FONSECA, Fernando Luiz Afonso; NUCCI, Ricardo Aparecido Baptista; MAIFRINO, Laura Beatriz MesianoWith the advancing age, there is an increase of the incidence of chronic renal diseases. However, it is suggested that exercise, as resistance training, is a key factor in positively modulate the kidney structure and function. Thus, this study aimed to analyze the effects of estrogen deprivation and a moderate resistance protocol on the right kidney structure of rats. A total of 32 animals were randomly divided in four groups (n = 8/per group): control group (CG); resistance trained animals (RT); control ovariectomized animals (OvxC); and trained ovariectomized animals (OvxT). Sections of the right kidney were stained with hematoxylin and eosin for morphometry and blood was collected for biochemical analysis. Our results showed that the group submitted to both ovariectomy and resistance training had significant impact on renal corpuscle components (Bowman's space, renal corpuscle area, and visceral layer), and proximal and distal tubules. Additionally, resistance training, with or without estrogen deprivation, increases creatine phosphokinase and decreases ultrasensitive C-reactive protein. In summary, we suggested that moderate resistance training may be a key factor to maintain the kidney structure and function across age. However, future studies are need to corroborate with our findings.
- Age-related changes in the articular cartilage of the mandible of rats(2020) CALDERON, M. F.; NUCCI, R. A. B.; SOUZA, R. R. deThis study investigated the effects of aging on the articular cartilage of the mandible. Wistar rats were divided in two groups (n = 10/per group): 3-months-old group (young group); and 13-months-old group (aged group). After euthanasia, the head of the mandible was collected and stained with hematoxylin and eosin (HE) to evaluate the thickness of the articular layer and cartilage. Sections stained with Picrosirius red and Safranin O were used to evaluate the collagen and proteoglycans deposition, respectively. First, aging has decreased the articular layer thickness. Second, the results suggest a decrease of chondrocytes followed by an increase of the matrix to maintain the mandible homeostasis. Finally, both collagen and proteoglycans increased with aging. Aging displayed important effects to the mandible of aged rats.
- Diaphragm muscle structure in the elderly: Findings from an autopsy study(2020) NUCCI, Ricardo Aparecido Baptista; SOUZA, Romeu Rodrigues de; SUEMOTO, Claudia Kimie; BUSSE, Alexandre Leopold; MAIFRINO, Laura Beatriz Mesiano; ANARUMA, Carlos Alberto; PASQUALUCCI, Carlos Augusto; JACOB-FILHO, WilsonSarcopenia is characterized as aging-related weakness and atrophy, which decreases the diaphragm force generating capacity leading to a decrease of expulsive non-ventilatory motor behaviors, which are critical for airway clearance. Thus, this study aimed to analyze the histopathology of the diaphragm in postmortem samples. Thirty individuals were included. Diaphragm samples were stained with hematoxylin and eosin for histopathological analysis. Picrosirius stain was used to highlight the collagen fibers. We observed a positive association between advancing age and histopathological findings in the diaphragm structure. We suggest that age is a key factor in increasing diaphragm muscle histopathology. However, further clinicopathological studies are needed to confirm our findings.
- Effects of moderate aerobic exercise on thoracic aortic remodeling of female LDL-receptor knockout ovariectomized mice(2020) LIMA, Nathalia Edviges Alves de; BRIANEZI, Ledimar; NUCCI, Ricardo Aparecido Baptista; CARDOSO, Clever Gomes; MAGALHAES, Walkyria Villegas; CHUCATA, Kemily Loren Barros; FONSECA, Fernando Luiz Afonso; MAIFRINO, Laura Beatriz MesianoMenopause is a major factor involved in dyslipidemia increasing the risk of atherosclerosis which may be reversed by a routine of aerobic physical activity. Thus, this study aimed to analyze the effects of aerobic training on the thoracic aorta of female LDL-receptor knockout mice submitted to estrogen deprivation. Fifteen genetically modified female mice, knockout for the low-density lipoprotein receptor (LDL-Knockout group) were used as experimental groups and fifteen wild female mice (C57BL/6 J) were used as control groups. Animals were divided as (n = 5/per group): sedentary control (SC); sedentary control ovariectomized (SCO); trained control ovariectomized (TCO); LDL-Knockout sedentary (KS); LDL-Knockout sedentary ovariectomized (KOS); and LDL-Knockout trained ovariectomized (KOT). Immunohistochemical techniques for TIMP-1 and metalloproteinases 2 and 9 were used to evaluate thoracic aorta remodeling. Picrosirius stain was used to highlight the collagen fibers. Verhoff-Van Gienson was used for the quantitative analyses of elastic lamellae. Our results demonstrate a positive remodeling promoted by physical exercise in ovariectomized and dyslipidemic animals. However, further studies are needed including the evaluation of inflammatory markers present in dyslipidemia.