TERESA YAE TAKAGAKI
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
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conferenceObject Immune Profiling Data and Mutational Status Improves Prediction of Risk of Death in Non-Small Cell Lung Carcinoma(2019) PARRA, E.; JANG, M.; MACHADO-RUGOLO, J.; FARHAT, C.; NAGAI, M.; TAKAGAKI, T.; TERRA, R.; FABRO, A.; CAPELOZZI, V.conferenceObject Hyaluronan and its impact in the screening and diagnosis of lung cancer patients(2012) RANGEL, M. P.; SA, V. K. de; MARTINS, J. R. Maciel; PARRA, E. R.; TAKAGAKI, T.; LONGATTO FILHO, A.; REIS, R.; CARRARO, D. M.; CAPELOZZI, V. L.Introduction: Hyaluronic Acid (HA) concentration is elevated in several cancers, but there is no data regarding its concentration related to lung cancer. In this study, we examined the HA concentrations in the tissue and in the sputum of lung cancer patients and its impact on the screening and diagnosis of the disease. Materials and Methods: HA was examined in tissue samples of 45 patients and sputum samples of 90 lung cancer patients. The controls were 25 COPD patients and 15 healthy controls. All the patients and controls underwent a sputum induction. Tissue and sputum samples were incubated with a proteolytic enzyme. The levels of HA were measured by a noncompetitive ELISA-like fluorometric assay. Results: A significant different concentration pattern of HA in the tissue was found between tumoral and non-tumoral samples (P < 0.001). Equally significant was the difference found in the sputum among lung cancer, COPD and healthy individuals (P < 0.001). ROC curve between lung cancer and healthy volunteers furnished an area of 0.821. Assuming a cut-off value of 31.44 ng/mg, the specificity was 100% and the sensitivity was 51%. ROC curve to distinguish COPD patients from lung cancer patients showed an area of 0.698 and the cut-off value of 48.3 ng/mg presented 100% specificity and 33% sensitivity. Conclusion: The results presented suggest a possible role of HA on lung cancer development as well as a promising role as a novel screening and diagnostic marker in the sputum for differentiating normal from lung cancer patients.conferenceObject Assessment of PDL1 and Immunoprofiling Using Multiplex Quantitative Immunofluorescence in Lung Cancer: Clinical Implications(2017) PRIETO, T.; FAHRAT, C.; TAKAGAKI, T.; RODRIGUEZ-CANALES, J.; WISTUBA, I.; CAPELOZZI, V.; CUENTAS, E. ParraconferenceObject DLL-3 and ASCL-1 Expression Emerge as Promising Therapeutic Targets in High-Grade Neuroendocrine Lung Tumors: A Preliminary Study(2021) PRIETO, T.; BALDAVIRA, C. Machado; ABSABER, A.; TAKAGAKI, T.; CAPELOZZI, V.conferenceObject Mutation profile and protein expression for predictive testing in lung adenocarcinoma: A study of 200 patients from Brazil(2016) MACHADO, J. R.; FABRO, A. T.; ASCHERI, D.; LEAO, P. d. Santos; SANTOS, A. L. dos; SA, V. K. de; RAINHO, C. A.; CUENTAS, E. R. Para; TAKAGAKI, T.; CAPELOZZI, V. L.conferenceObject Clinical Relevant Oncogenic Drivers in Advanced Adenocarcinoma Discloses New Therapeutic Targets in Negative EGFR/ALK/KRAS Patients(2017) MACHADO, Juliana; ASCHERI, Daniel; LEAO, Patricia; MILSONI, Priscila; OLIVEIRA, Rogerio; PARRA, Edwin; SA, Vanessa; FARHAT, Cecilia; RAINHO, Claudia; AB'SABER, Alexandre; NAGAI, Maria; TAKAGAKI, Teresa; FABRO, Alexandre; CAPELOZZI, VeraconferenceObject Hyaluronan: A novel sputum marker for the screening and diagnosis of lung cancer patients(2012) RANGEL, Maristela Peres; SA, Vanessa Karen de; MARTINS, Joao Roberto Maciel; CUENTAS, Edwin Roger Parra; REIS, Rui Vieira; LONGATTO FILHO, Adhemar; TAKAGAKI, Tereza; NADER, Helena; CAPELOZZI, Vera LuizaconferenceObject Protein and Molecular Alterations in EMT Pathways of Lung Cancer: A Comparative Analysis between NSCLCs(2017) MACHADO, Juliana; ASCHERI, Daniel; LEAO, Patricia; MILSONI, Priscila; OLIVEIRA, Rogerio; PARRA, Edwin; SA, Vanessa; FARHAT, Cecilia; AB'SABER, Alexandre; NAGAI, Maria; TAKAGAKI, Teresa; FABRO, Alexandre; CAPELOZZIL, VeraconferenceObject SPUTUM HYALURONAN ANALYSIS AS A NEW STRATEGY FOR THE SCREENING AND DIAGNOSIS OF LUNG CANCER(2012) RANGEL, Maristela P.; SA, Vanessa K. De; MARTINS, Joao Roberto M.; CUENTAS, Edwin Roger P.; TAKAGAKI, Tereza; FILHO, Adhemar L.; REIS, Rui; NADER, Helena; CAPELOZZI, Vera L.Background: Hyaluronic Acid (HA) concentration is elevated in several cancers, but there is no data regarding its concentration in the sputum of lung cancer patients.In this study, we examined the HA concentrations in the tissue and in the sputum of lung cancer patients and its impact on the screening and diagnosis of the disease. Methods: HA was examined in 45 paired tissues samples and sputum samples were collected from 90 lung cancer patients. Twenty five COPD patients were selected to compose a high risk group and 15 healthy volunteers were analysed as controls. All the patients and controls underwent a sputum induction. Sputum samples were incubated with urea 7M and tissue samples were dehydrated with acetone prior to analysis. Afterwards both were incubated with a proteolytic enzyme. The levels of HA were measured by a noncompetitive ELISA-like fluorometric assay. Results and Conclusion: A significant different concentration pattern of HA in the tissues was found between tumoral and non-tumoral samples (p<0.001). Equally significant was the difference found in the sputum among lung cancer, COPD and healthy individuals (p<0.001 Fig1A). When comparing the groups separately, a difference was found between sputum HA from LC vs.healthy volunteers (p<0.001) and LC vs.COPD patients (p=0.002). ROC curve between LC and healthy volunteers furnished an area of 0.821. Assuming a cut off value of 31,44ng/mg, the specificity was 100% and the sensitivity was 51% (Fig1B). ROC curve to distinguish COPD patients from LC patients showed an area of 0.698 and the cut off value of 48.3ng/mg presented 100% of specificity and 33% of sensitivity.