CLAUDIA GOLDENSTEIN SCHAINBERG
Projetos de Pesquisa
Unidades Organizacionais
LIM/17 - Laboratório de Investigação em Reumatologia, Hospital das Clínicas, Faculdade de Medicina
13 resultados
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bookPart Lúpus eritematoso sistêmico juvenil(2021) AIKAWA, Nádia Emi; ASSAD, Ana Paula Luppino; SCHAINBERG, Cláudia Goldenstein; PEREIRA, Rosa Maria Rodrigues- Identification of Autoimmunity to Peptides of Collagen V alpha 1 Chain as Newly Biomarkers of Early Stage of Systemic Sclerosis(2021) VELOSA, Ana Paula Pereira; BRITO, Lais; QUEIROZ, Zelita Aparecida de Jesus; CARRASCO, Solange; MIRANDA, Jurandir Tomaz de; FARHAT, Cecilia; GOLDENSTEIN-SCHAINBERG, Claudia; PARRA, Edwin Roger; ANDRADE, Danieli Castro Oliveira de; SILVA, Pedro Leme; CAPELOZZI, Vera Luiza; TEODORO, Walcy RosoliaPatients with Systemic sclerosis (SSc) presents immune dysregulation, vasculopathy, and fibrosis of the skin and various internal organs. Pulmonary fibrosis leads to SSc-associated interstitial lung disease (ILD), which is the main cause of morbidity and mortality in SSc. Recently autoimmunity to type V collagen (Col V) has been characterized in idiopathic pulmonary fibrosis and show promise to be related to the development in SSc. Our aim was to evaluate autoimmunity to Col V alpha 1(V) and alpha 2(V) chains and to the antigenic peptides of these Col V chains in early-SSc sera employing lung tissue of SSc-ILD, as antigen source. We found that sera samples from patients with early-SSc were reactive to Col V (41.18%) and presented immunoreactivity for Col5A1(1.049) and Col5A1(1.439) peptides. The IgG isolated from early-SSc patients-anti-Col V positive sera (anti-ColV IgG) was adsorbed with alpha 1(V) chain (anti-ColV IgG/ads-alpha 1(V)) and alpha 2(V) chain (anti-ColV IgG/ads-alpha 2(V)) and biotinylated to evaluate the spectrum of reactivity in SSc-ILD patients lung biopsies by immunofluorescence. The SSc-ILD lung tissue samples immunostained with anti-ColV IgG showed increased green fluorescence in the vascular basement membrane, bronchiolar smooth muscle, and adventitial layer, contrasting with the tenue immunostaining in control lungs. Col V protein expression in these pulmonary compartments immunostained with early-SSc anti-ColV IgG was confirmed by immune colocalization assays with commercial anti-human Col V antibodies. In addition, SSc-ILD lung tissues immunostained with anti-ColV IgG/ads-alpha 1(V) (sample in which Col V alpha 1 chain-specific antibodies were removed) showed decreased green fluorescence compared to anti-ColV IgG and anti-ColV IgG/ads-alpha 2(V). Our data show that autoimmunity to Col V in early-SSc was related to peptides of the alpha 1(V) chain, suggesting that these antibodies could be biomarkers of SSc stages and potential target of immunotherapy with Col V immunogenic peptides.
- Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage(2021) CRUZ, Isabele Camargo Brindo da; VELOSA, Ana Paula Pereira; CARRASCO, Solange; SANTOS FILHO, Antonio dos; MIRANDA, Jurandir Tomaz de; POMPEU, Eduardo; FERNANDES, Tiago Lazzaretti; BUENO, Daniela Franco; FANELLI, Camila; GOLDENSTEIN-SCHAINBERG, Claudia; FABRO, Alexandre Todorovic; FULLER, Ricardo; SILVA, Pedro Leme; CAPELOZZI, Vera Luiza; TEODORO, Walcy RosoliaCollagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment.
bookPart Artropatias Microcristalinas(2021) FULLER, Ricardo; SILVA, Henrique Carriço da; SCHAINBERG, Cláudia Goldenstein- Brazilian Society of Rheumatology 2020 guidelines for psoriatic arthritis(2021) CARNEIRO, Sueli; PALOMINOS, Penelope Esther; ANTI, Sonia Maria Alvarenga; ASSAD, Rodrigo Luppino; GONCALVES, Rafaela Silva Guimaraes; CHIEREGHIN, Adriano; LYRIO, Andre Marun; XIMENES, Antonio Carlos; SAAD, Carla Goncalves; GONCALVES, Celio Roberto; KOHEM, Charles Lubianca; MARQUES, Claudia Diniz Lopes; SCHAINBERG, Claudia Goldenstein; MEIRELLES, Eduardo de Souza; RESENDE, Gustavo Gomes; PIERUCCETTI, Lenise Brandao; KEISERMAN, Mauro Waldemar; YAZBEK, Michel Alexandre; SAMPAIO-BARROS, Percival Degrava; LAGE, Ricardo da Cruz; BONFIGLIOLI, Rubens; OLIVEIRA, Thauana Luiza; AZEVEDO, Valderilio Feijo; BIANCHI, Washington Alves; BERNARDO, Wanderley Marques; SIMOES, Ricardo dos Santos; PINHEIRO, Marcelo de Medeiros; CAMPANHOLO, Cristiano BarbosaPsoriatic arthritis (PsA) is a chronic and systemic immune disease characterized by inflammation of peripheral and/or axial joints and entheses in patients with psoriasis (PsO). Extra-articular and extracutaneous manifestations and numerous comorbidities can also be present. These recommendations replace the previous version published in May 2013. A systematic review of the literature retrieved 191 articles that were used to formulate 12 recommendations in response to 12 clinical questions, divided into 4 sections: diagnosis, non-pharmacological treatment, conventional drug therapy and biologic therapy. These guidelines provide evidence-based information on the clinical management for PsA patients. For each recommendation, the level of evidence (highest available), degree of strength (Oxford) and degree of expert agreement (interrater reliability) are reported.
bookPart Esclerose sistêmica juvenil e escleodermia localizada juvenil(2021) AIKAWA, Nádia Emi; ASSAD, Ana Paula Luppino; SCHAINBERG, Cláudia Goldenstein; PEREIRA, Rosa Maria RodriguesconferenceObject PREDICTORS OF BIOLOGIC THERAPY AND SYNDESMOPHYTE FORMATION IN PATIENTS WITH NON-RADIOGRAPHIC AXIAL SPONDYLOARTHRITIS(2021) BARRETO-NETO, N.; ARAUJO-BRANDAO, L.; RIBEIRO, A. C. M.; MORAES, J. C. B.; GOLDENSTEIN-SCHAINBERG, C.; SHIMABUCO, A. Y.; GONCALVES, C. R.; SAMPAIO-BARROS, P. D.; SAAD, C. G. S.- Brazilian recommendations for the use of nonsteroidal anti-inflammatory drugs in patients with axial spondyloarthritis(2021) LAGE, Ricardo da Cruz; MARQUES, Claudia Diniz Lopes; OLIVEIRA, Thauana Luiza; RESENDE, Gustavo Gomes; KOHEM, Charles Lubianca; SAAD, Carla Goncalves; XIMENES, Antonio Carlos; GONCALVES, Celio Roberto; BIANCHI, Washington Alves; MEIRELLES, Eduardo de Souza; KEISERMAN, Mauro Waldemar; CHIEREGHIN, Adriano; CAMPANHOLO, Cristiano Barbosa; LYRIO, Andre Marun; SCHAINBERG, Claudia Goldenstein; PIERUCCETTI, Lenise Brandao; YAZBEK, Michel Alexandre; PALOMINOS, Penelope Esther; GONCALVES, Rafaela Silva Guimaraes; ASSAD, Rodrigo Luppino; BONFIGLIOLI, Rubens; LIMA, Sonia Maria Alvarenga Anti Loduca; CARNEIRO, Sueli; AZEVEDO, Valderilio Feijo; ALBUQUERQUE, Cleandro Pires; BERNARDO, Wanderley Marques; SAMPAIO-BARROS, Percival Degrava; PINHEIRO, Marcelo de MedeirosSpondyloarthritis (SpA) is a group of chronic inflammatory systemic diseases characterized by axial and/or peripheral joints inflammation, as well as extra-articular manifestations. Over some decades, nonsteroidal anti-inflammatory drugs (NSAIDs) have been the basis for the pharmacological treatment of patients with axial spondyloarthritis (axSpA). However, the emergence of the immunobiologic agents brought up the discussion about the role of NSAIDs in the management of these patients. The objective of this guideline is to provide recommendations for the use of NSAIDs for the treatment of axSpA. A panel of experts from the Brazilian Society of Rheumatology conducted a systematic review and meta-analysis of randomized clinical trials for 15 predefined questions. The Grading of Recommendations, Assessment, Development and Evaluation methodology to assess the quality of evidence and formulate recommendations were used, and at least 70% agreement of the voting panel was needed. Fourteen recommendations for the use of NSAIDs in the treatment of patients with axSpA were elaborated. The purpose of these recommendations is to support clinicians' decision making, without taking out his/her autonomy when prescribing for an individual patient.
bookPart Febre reumática(2021) ASSAD, Ana Paula Luppino; AIKAWA, Nádia Emi; SCHAINBERG, Cláudia Goldenstein; PEREIRA, Rosa Maria RodriguesbookPart Artrite idiopática juvenil(2021) SCHAINBERG, Cláudia Goldenstein; ASSAD, Ana Paula Luppino; AIKAWA, Nádia Emi; PEREIRA, Rosa Maria Rodrigues