ANTONIO CHARLYS DA COSTA
Projetos de Pesquisa
Unidades Organizacionais
LIM/52 - Laboratório de Virologia, Hospital das Clínicas, Faculdade de Medicina
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- A new gyrovirus in human feces(2015) PHAN, Tung Gia; COSTA, Antonio Charlys da; ZHANG, Wen; POTHIER, Pierre; AMBERT-BALAY, Katia; DENG, Xutao; DELWART, EricA novel gyrovirus genome found in the feces of an adult with diarrhea is described. The genome shows the three expected main ORFs encoding a structural protein (VP1), nonstructural protein (VP2), and Apoptin protein (VP3), which shared identities of 41, 42, and 38 % with those of the most closely related gyrovirus proteins, respectively. Given the high divergence in its genome, this gyrovirus may be considered the prototype for a new viral species (GyV9) in the Gyrovirus genus. Because the closest relatives of this gyrovirus infect chicken, a possible dietary origin for the presence of this virus in human feces is discussed.
- Wuhan large pig roundworm virus identified in human feces in Brazil(2018) LUCHS, Adriana; LEAL, Elcio; KOMNINAKIS, Shirley Vasconcelos; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; GILL, Danielle Elise; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; COSTA, Antonio Charlys daWe report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.
- Aedes aegypti Totivirus identified in mosquitoes in the Brazilian Amazon region(2023) LEAL, Elcio; RIBEIRO, Edcelha Soares D'Athaide; MONTEIRO, Fred Julio Costa; MARQUES, Julia Pantoja; MENDES, Diuliana dos Santos; MORAIS, Vanessa S.; ARAUJO, Emerson Luiz Lima; PANDEY, Ramendra Pati; CHANG, Chung-Ming; DENG, Xutao; DELWART, Eric; COSTA, Antonio Charlys da; LIMA, KledoaldoThe totiviridae family contains viruses with double-stranded RNA genomes of 4.6-7.0 kpb, which encode a capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), and they are approximately 40 nm in diameter with icosahedral symmetry. Totiviruses were first isolated from mosquitoes collected in Shaanxi Province (China). Here, we report a new Aedes aegypti Totivirus (AaTV) identified in mosquitoes from the Amazon rainforest. Mosquitoes (Diptera: Culicidae) were collected from a forest reserve belonging to the Amazon forest in the city of Macapa, Amapa state, Northern Brazil. A viral sequence with a 5748 nucleotide length that was nearly identical to Aedes aegypti Totivirus (AaTV), here named Aedes aegypti Totivirus BR59AP, was detected. A detailed molecular analysis was performed and shows that AaTV-BR59AP is highly related to the AaTV strain from the Caribbean region. We emphasize the importance of the characterization of new viruses in mosquitoes to deepen our understanding of viral diversity in insects and their potential role in disease.
- Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil(2019) COSTA, Antonio Charlys da; LEAL, Elcio; GILL, Danielle; MILAGRES, Flavio Augusto de Padua; KOMNINAKIS, Shirley Vasconcelos; BRUSTULIN, Rafael; TELES, Maria da Aparecida Rodrigues; LOBATO, Marcia Cristina Alves Brito Sayao; CHAGAS, Rogerio Togisaki das; ABRAO, Maria de Fatima Neves dos Santos; SOARES, Cassia Vitoria de Deus Alves; DENG, Xutao; DELWART, Eric; SABINO, Ester Cerdeira; LUCHS, AdrianaThe nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Metagenomic analyses offered an opportunity to identify for the first time in Brazil a newly described endornavirus species.
- Characterization of Phietavirus Henu 2 in the virome of individuals with acute gastroenteritis(2023) RAMOS, Endrya do Socorro Foro; BAHIA, Santana Lobato; RIBEIRO, Geovani de Oliveira; VILLANOVA, Fabiola; MILAGRES, Flavio Augusto de Padua; BRUSTULIN, Rafael; PANDEY, Ramendra Pati; DENG, Xutao; DELWART, Eric; COSTA, Antonio Charlys da; LEAL, ElcioThere is a growing interest in phages as potential biotechnological tools in human health owing to the antibacterial activity of these viruses. In this study, we characterized a new member (named PhiV_005_BRA/2016) of the recently identified phage species Phietavirus Henu 2. PhiV_005_BRA/2016 was detected through metagenomic analysis of stool samples of individuals with acute gastroenteritis. PhiV_005_BRA/2016 contains double-stranded linear DNA (dsDNA), it has a genome of 43,513 base pairs (bp), with a high identity score (99%) with phage of the genus Phietavirus, species of Phietavirus Henu 2. Life style prediction indicated that PhiV_005_BRA/2016 is a lysogenic phage whose the main host is methicillin-resistant Staphylococcus aureus (MRSA). Indeed, we found PhiV_005_BRA/2016 partially integrated in the genome of distinct MRSA strains. Our findings highlights the importance of large-scale screening of bacteriophages to better understand the emergence of multi-drug resistant bacterial.