BRUNO FORTALEZA DE AQUINO FERREIRA

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Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/33 - Laboratório de Oftalmologia, Hospital das Clínicas, Faculdade de Medicina

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Agora exibindo 1 - 6 de 6
  • article 10 Citação(ões) na Scopus
    Bactec (TM) blood culture bottles allied to MALDI-TOF mass spectrometry: rapid etiologic diagnosis of bacterial endophthalmitis
    (2017) TANAKA, Tatiana; OLIVEIRA, Luiza Manhezi de Freitas; FERREIRA, Bruno Fortaleza de Aquino; KATO, Juliana Mika; ROSSI, Flavia; CORREA, Karoline de Lemes Giuntini; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce Hisae; ALMEIDA JUNIOR, Joao Nobrega
    Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been used for direct identification of pathogens from blood-inoculated blood culture bottles (BCBs). We showed that MALDI-TOF MS is an useful technique for rapid identification of the causative agents of endophthalmitis from vitreous humor-inoculated BCBs with a simple protocol.
  • conferenceObject
    Explanted Scleral Buckle, a retrospective study
    (2015) OLIVEIRA, Luiza Manhezi de Freitas; GIOIA, Thais Sabota Romano Di; ROSA, Valeria Teixeira Alves; ALMEIDA, Joao Nobrega de Nobrega de; ROSSI, Flavia; KATO, Juliana Mika; FERREIRA, Bruno Fortaleza de Aquino; TANAKA, Tatiana; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti
  • conferenceObject
    The use of pediatric blood culture bottles in the diagnosis of acute postoperative endophthalmitis
    (2015) TANAKA, Tatiana; ALMEIDA, Joao Nobrega de; GIOIA, Thais Sabota Romano Di; ROSSI, Flavia; KATO, Juliana Mika; FERREIRA, Bruno Fortaleza de Aquino; RUPPERT, Aline Domingos Pinto; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce H.
  • conferenceObject
    Bactec (TM) blood culture bottles allied to MALDI-TOF mass spectrometry: Rapid etiologic diagnosis of bacterial endophthalmitis
    (2017) TANAKA, Tatiana; OLIVEIRA, Luiza Manhezi de Freitas; FERREIRA, Bruno Fortaleza de Aquino; KATO, Juliana Mika; ROSSI, Flavia; CORREA, Karoline de Lemes Giuntini; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce H.; ALMEIDA JUNIOR, Joao Nobrega de
  • article 4 Citação(ões) na Scopus
    Diagnostic value of pediatric blood culture bottles for acute postoperative endophthalmitis
    (2019) TANAKA, Tatiana; FERREIRA, Bruno Fortaleza de Aquino; OLIVEIRA, Luiza Manhezi Shin de; KATO, Juliana Mika; GIOIA, Thais Sabato Romano Di; ROSSI, Flavia; NAKASHIMA, Yoshitaka; PIMENTEL, Sergio Luis Gianotti; YAMAMOTO, Joyce Hisae; ALMEIDA JUNIOR, Joao Nobrega de
    OBJECTIVE: To report our experience using conventional culture methods (CM) and pediatric blood culture bottles (PBCBs) for vitreous sample culture of acute postoperative endophthalmitis. METHODS: A retrospective study was conducted at the Department of Ophthalmology, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, BR, from January 2010 to December 2015, and it included 54 patients with clinically suspected acute postoperative endophthalmitis. Vitreous samples were obtained by vitreous tap or vitrectomy. Samples from January 2010 to December 2011 were cultivated in CM, whereas samples from January 2012 to December 2015 were inoculated in PBCBs. The measured outcome was the yield of positive cultures. RESULTS: Twenty cases were included in the CM group, and 34 cases were included in the PBCB group. The yield of positive cultures in PBCBs (64.7%) was significantly higher than that in conventional CM (35%, p=0.034). Staphylococcus epidermidis and Streptococcus viridans were the two most commonly found agents. CONCLUSION: PBCBs can be used successfully in clinically suspected endophthalmitis. The method showed a higher yield of positive cultures than the conventional method. This technique appears to have several advantages over the traditional method: it saves time, as only one medium needs to be inoculated; transportation to a laboratory is easier than in the traditional method, and there is no need to maintain a supply of fresh agar media. The use of PBCBs may be recommended as the primary method for microbiological diagnosis and is especially suitable for office settings and remote clinics.
  • bookPart
    Exame Oftalmológico para o Clínico Geral
    (2016) ALMEIDA, Augusto Cesar Costa de; FERREIRA, Bruno Fortaleza de Aquino; GABRIEL, Gustavo Mori; VIANNA, Jayme Augusto Rocha; TANAKA, Tatiana