DANIELA APARECIDA DE BRITO CERVILHA
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Regulatory T cells in COPD development: How the animal model resembles the human pathophysiological features(2017) ITO, Juliana Tiyaki; CERVILHA, Daniela Aparecida de Brito; SILVA, Larissa Emidio de Franca; LOURENCO, Juliana Dias; MOREIRA, Alyne Riani; KOHLER, Julia Benini; NEGRI, Elnara Marcia; MACCHIONE, Mariangela; MAUAD, Thais; MARTINS, Milton Arruda; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos
- A murine model of elastase and cigarette smoke induced emphysema(2017) RODRIGUES, Rubia; OLIVO, Clarice Rosa; LOURENCO, Juliana Dias; RIANE, Alyne; CERVILHA, Daniela Aparecida de Brito; LOPES, Fernanda Degobbi Tenrio Quirino dos SantosObjective: To describe a murine model of emphysema induced by a combination of exposure to cigarette smoke (CS) and instillation of porcine pancreatic elastase (PPE). Methods: A total of 38 C57BL/6 mice were randomly divided into four groups: control (one intranasal instillation of 0.9% saline solution); PPE (two intranasal instillations of PPE); CS (CS exposure for 60 days); and CS + PPE (two intranasal instillations of PPE + CS exposure for 60 days). At the end of the experimental protocol, all animals were anesthetized and tracheostomized for calculation of respiratory mechanics parameters. Subsequently, all animals were euthanized and their lungs were removed for measurement of the mean linear intercept (Lm) and determination of the numbers of cells that were immunoreactive to macrophage (MAC)-2 antigen, matrix metalloproteinase (MMP)-12, and glycosylated 91-kDa glycoprotein (gp91phox) in the distal lung parenchyma and peribronchial region. Results: Although there were no differences among the four groups regarding the respiratory mechanics parameters assessed, there was an increase in the Lm in the CS + PPE group. The numbers of MAC-2-positive cells in the peribronchial region and distal lung parenchyma were higher in the CS + PPE group than in the other groups, as were the numbers of cells that were positive for MMP-12 and gp91phox, although only in the distal lung parenchyma. Conclusions: Our model of emphysema induced by a combination of PPE instillation and CS exposure results in a significant degree of parenchymal destruction in a shorter time frame than that employed in other models of CS-induced emphysema, reinforcing the importance of protease-antiprotease imbalance and oxidant-antioxidant imbalance in the pathogenesis of emphysema.
- Regulatory T-Cell Distribution within Lung Compartments in COPD(2017) SALES, Davi S.; ITO, Juliana T.; ZANCHETTA, Ivy A.; ANNONI, Raquel; AUN, Marcelo V.; FERRAZ, Luiz Fernando S.; CERVILHA, Daniela A. B.; NEGRI, Elnara; MAUAD, Thais; MARTINS, Milton A.; LOPES, Fernanda D. T. Q. S.The importance of the adaptive immune response, specifically the role of regulatory T (Treg) cells in controlling the obstruction progression in smokers, has been highlighted. To quantify the adaptive immune cells in different lung compartments, we used lung tissues from 21 never-smokers without lung disease, 22 current and/or ex-smokers without lung disease (NOS) and 13 current and/or ex-smokers with chronic obstructive pulmonary disease (COPD) for histological analysis. We observed increased T, B, IL-17 and BAFF(+) cells in small and large airways of COPD individuals; however, in the NOS, we only observed increase in T and IL-17(+) cells only in small airways. A decrease in the density of Treg(+), TGF-beta(+) and IL-10(+) in small and large airways was observed only in COPD individuals. In the lymphoid tissues, Treg, T,B-cells and BAFF(+) cells were also increased in COPD; however, changes in Treg inhibitory associated cytokineswere not observed in this compartment. Therefore, our results suggest that difference in Treg(+) cell distributions in lung compartments and the decrease in TGF-beta(+) and IL-10(+) cells in the airways may lead to the obstruction in smokers.
- Different microenvironment stimuli results in distinct patterns of macrophages polarization comparing elastase- and cigarette smoke - induced models of emphysema(2017) KOHLER, Julia Benini; CERVILHA, Daniela A. B.; ITO, Juliana T.; MOREIRA, Alyne R.; SANTANA, Fernanda R.; FARIAS, Talita M.; ALONSO-VALE, Maria Isabel C.; PRADO, Carla M.; MARTINS, Milton Arruda; LOPES, Fernanda D. T. Q. S.; FRANCA, Larissa E.