THAYSE REGINA BRUGGEMANN
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
conferenceObject Bordetella Pertussis Whole-Cell Vaccine Inhibits Specific IgE, Inflammation and Airway Remodeling in a Murine Model of Asthma(2015) AUN, Marcelo Vivolo; ARANTES-COSTA, Fernanda; SARAIVA-ROMANHOLO, Beatriz Mangueira; ALMEIDA, Francine Maria; REGINA-BRUEGGERMANN, Thayse; MARTINS, Milton Arruda; KALIL, Jorge; GIAVINA-BIANCHI, Pedro- Effects of Swimming on the Inflammatory and Redox Response in a Model of Allergic Asthma(2015) BRUEGGEMANN, T. R.; AVILA, L. C. M.; FORTKAMP, B.; GREIFFO, F. R.; BOBINSKI, F.; MAZZARDO-MARTINS, L.; MARTINS, D. F.; DUARTE, M. M. M. F.; DAFRE, A.; SANTOS, A. R. S.; SILVA, M. D.; SOUZA, L. F.; VIEIRA, R. P.; HIZUME-KUNZLER, D. C.In this study we hypothesized that swimming during sensitization phase could result in a preventive effect in mice with allergic asthma. Swiss mice were divided into 4 groups: Control and Swimming (non-sensitized), OVA and OVA+Swimming (sensitized). The allergic inflammation was induced by 2 intraperitoneal injections and 4 aerosol challenges using ovalbumin. Swimming sessions were performed at high intensity over 3 weeks. 48h after the last challenge mice were euthanized. Swimming decreased OVA-increased total IgE, IL-1, IL-4, IL-5 and IL-6 levels, as well as the number of total cells, lymphocytes and eosinophils in bronchoalveolar lavage fluid, (p<0.05). Simultaneously, swimming also increased IL-10 and glutathione levels in the Swimming and OVA+Swimming groups (p<0.05). The levels of glutathione peroxidase and catalase were increased only in the Swimming group when compared to all groups (p<0.05). 21 days of swimming resulted in an attenuation of pulmonary allergic inflammation followed by an increase of glutathione levels in the OVA group. Swimming only increased the levels of glutathione peroxidase and catalase in non-sensitized mice (p<0.05). These data suggest that the pulmonary anti-inflammatory effects produced by 3 weeks of high-intensity swimming in this model of OVA-induced asthma may be, at least partly, modulated by reduced oxidative stress and increased IL-10 production.
- Effects of High-Intensity Swimming on Lung Inflammation and Oxidative Stress in a Murine Model of DEP-Induced Injury(2015) AVILA, Leonardo C. M.; BRUGGEMANN, Thayse R.; BOBINSKI, Franciane; SILVA, Morgana Duarte da; OLIVEIRA, Regiane Carvalho; MARTINS, Daniel Fernandes; MAZZARDO-MARTINS, Leidiane; DUARTE, Marta Maria Medeiros Frescura; SOUZA, Luiz Felipe de; DAFRE, Alcir; VIEIRA, Rodolfo de Paula; SANTOS, Adair Roberto Soares; BONORINO, Kelly Cattelan; KUNZLER, Deborah de C. HizumeStudies have reported that exposure to diesel exhaust particles (DEPs) induces lung inflammation and increases oxidative stress, and both effects are susceptible to changes via regular aerobic exercise in rehabilitation programs. However, the effects of exercise on lungs exposed to DEP after the cessation of exercise are not clear. Therefore, the aim of this study was to evaluate the effects of high-intensity swimming on lung inflammation and oxidative stress in mice exposed to DEP concomitantly and after exercise cessation. Male Swiss mice were divided into 4 groups: Control (n = 12), Swimming (30 min/day) (n = 8), DEP (3 mg/mL-10 mu L/mouse) (n = 9) and DEP+Swimming (n = 8). The high-intensity swimming was characterized by an increase in blood lactate levels greater than 1 mmoL/L between 10th and 30th minutes of exercise. Twenty-four hours after the final exposure to DEP, the anesthetized mice were euthanized, and we counted the number of total and differential inflammatory cells in the bronchoalveolar fluid (BALF), measured the lung homogenate levels of IL-1 beta, TNF-alpha, IL-6, INF-(sic), IL-10, and IL-1ra using ELISA, and measured the levels of glutathione, non-protein thiols (GSH-t and NPSH) and the antioxidant enzymes catalase and glutathione peroxidase (GPx) in the lung. Swimming sessions decreased the number of total cells (p<0.001), neutrophils and lymphocytes (p<0.001; p<0.05) in the BALF, as well as lung levels of IL-1 beta (p = 0.002), TNF-alpha (p = 0.003), IL-6 (p = 0.0001) and IFN-(sic) (p = 0.0001). However, the levels of IL-10 (p = 0.01) and IL-1ra (p = 0.0002) increased in the swimming groups compared with the control groups, as did the CAT lung levels (p = 0.0001). Simultaneously, swimming resulted in an increase in the GSH-t and NPSH lung levels in the DEP group (p = 0.0001 and p<0.002). We concluded that in this experimental model, the high-intensity swimming sessions decreased the lung inflammation and oxidative stress status during DEP-induced lung inflammation in mice.
- Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode(2015) AUN, Marcelo Vivolo; SARAIVA-ROMANHOLO, Beatriz Mangueira; ALMEIDA, Francine Maria de; BRüGGEMANN, Thayse Regina; KALIL, Jorge; MARTINS, Milton de Arruda; ARANTES-COSTA, Fernanda Magalhães; GIAVINA-BIANCHI, PedroABSTRACT Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.
- BmTI-A, a serine protease inhibitor, reduces the chronic allergic lung inflammation in a mice model(2015) FLORENCIO, Ariana; ARANTES-COSTA, Fernanda Magalhaes; ALMEIDA, Robson Santos de; BRUGGEMANN, Thayse Regina; SASAKI, Sergio D.; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.; LOPES, Fernanda D. T. Q. S.; LEICK, Edna A.
- Aerobic exercise reduces lung inflammation and fibrosis mediated by serotoninergic/Akt signaling in a model of pulmonary fibrosis(2015) VIEIRA, Rodolfo P.; ALMEIDA-OLIVEIRA, Ana Roberta; PEREIRA, Paulo R.; MATOS, Yves; OLIVEIRA-JUNIOR, Manoel C.; GREIFFO, Flavia R.; RIGONATO-OLIVEIRA, Nicole C.; ANDRADE-SOUSA, Adilson S.; BRUGEMMAN, Thayse R.; DELLE, Humberto; ALBERTINI, Regiane; OLIVEIRA, Ana Paula L. De; DAMACENO-RODRIGUES, Nilsa R.; FERNANDEZ, Isis E.; DOLHNIKOFF, Marisa; CASTRO-FARIA-NETO, Hugo C.; IDZKO, Marco; EICKELBERG, Oliver