VALERIA SUTTI NUNES
Projetos de Pesquisa
Unidades Organizacionais
LIM/10 - Laboratório de Lípides, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
conferenceObject Palmitic Interesterified Fat Induces Atherosclerosis and Inflammatory Cytokine Secretion in LDL Receptor Knockout Mice(2014) AFONSO, Milessa S.; LAVRADOR, Maria Silvia F.; KOIKE, Marcia; BOMBO, Renata P.; NUNES, Valeria S.; CATANOZI, Sergio; CASTILHO, Gabriela; PASSARELLI, Marisa; NAKANDAKARE, Edna R.; LOTTENBERG, Ana Maria- HDL Size is More Accurate than HDL Cholesterol to Predict Carotid Subclinical Atherosclerosis in Individuals Classified as Low Cardiovascular Risk(2014) PARRA, Eliane Soler; PANZOLDO, Natalia Baratella; ZAGO, Vanessa Helena de Souza; SCHERRER, Daniel Zanetti; ALEXANDRE, Fernanda; BAKKARAT, Jamal; NUNES, Valeria Sutti; NAKANDAKARE, Edna Regina; QUINTAO, Eder Carlos Rocha; NADRUZ- JR., Wilson; FARIA, Eliana Cotta de; SPOSITO, Andrei C.Background: Misclassification of patients as low cardiovascular risk (LCR) remains a major concern and challenges the efficacy of traditional risk markers. Due to its strong association with cholesterol acceptor capacity, high-density lipoprotein (HDL) size has been appointed as a potential risk marker. Hence, we investigate whether HDL size improves the predictive value of HDL-cholesterol in the identification of carotid atherosclerotic burden in individuals stratified to be at LCR. Methods and Findings: 284 individuals (40-75 years) classified as LCR by the current US guidelines were selected in a three-step procedure from primary care centers of the cities of Campinas and Americana, SP, Brazil. Apolipoprotein B-containing lipoproteins were precipitated by polyethylene glycol and HDL size was measured by dynamic light scattering (DLS) technique. Participants were classified in tertiles of HDL size (<7.57; 7.57-8.22; >8.22 nm). Carotid intima-media thickness (cIMT) <0.90 mm (80th percentile) was determined by high resolution ultrasonography and multivariate ordinal regression models were used to assess the association between cIMT across HDL size and levels of lipid parameters. HDLcholesterol was not associated with cIMT. In contrast, HDL size >8.22 nm was independently associated with low cIMT in either unadjusted and adjusted models for age, gender and Homeostasis Model Assessment 2 index for insulin sensitivity, ethnicity and body mass index (Odds ratio 0.23; 95% confidence interval 0.07-0.74, p=0.013). Conclusion: The mean HDL size estimated with DLS constitutes a better predictor for subclinical carotid atherosclerosis than the conventional measurements of plasma HDL-cholesterol in individuals classified as LCR.
conferenceObject A DIETARY TRANS FAT SUBSTITUTE ALSO INDUCES ATHEROSCLEROSIS DEVELOPMENT IN LDLR-KO MICE(2014) AFONSO, M. S.; LAVRADOR, M. S. F.; KOIKE, M. K.; BOMBO, R. P.; NUNES, V. S.; CATANOZI, S.; NAKANDAKARE, E. R.; LOTTENBERG, A. M.- Increased 27-hydroxycholesterol plasma level in men with low high density lipoprotein-cholesterol may circumvent their reduced cell cholesterol efflux rate(2014) NUNES, Valeria S.; PANZOLDO, Natalia B.; LEANCA, Camila C.; PARRA, Eliane S.; ZAGO, Vanessa S.; SILVA, Eliton J. da; CAZITA, Patricia M.; NAKANDAKARE, Edna R.; FARIA, Eliana C. de; QUINTAO, Eder C. R.Background: HDL is considered the most important mechanism for the excretion of intracellular cholesterol. The liver is the only organ capable to metabolize cholesterol into bile acid. The enzymatic conversion of cholesterol to bile acid is dependent on the cytochrome P450 microsomal system which is also responsible for the generation of oxysterols. The latter's plasma concentrations may reflect the metabolic processes of specific tissues where they are generated. The objective of this study was to investigate in healthy individuals who differ according to their HDL levels the concentration of oxysterols and relate it to the HDL-dependent cell cholesterol efflux rate. Methods: 24-Hydroxycholesterol, 25-hydroxycholesterol, 27-hydroxycholesterol were determined in plasma by GLC/mass spectrometry in 107 healthy subjects with low HDL (HDL-C < 1.03 mmol/l) and high HDL cholesterol (HDL-C > 1.55 mmol/l). HDL-dependent in vitro cell cholesterol efflux rate was measured in 29 cases. Results: No differences were found in plasma oxysterol concentrations between the Low HDL and High HDL groups. There was a significant negative correlation between HDL-C and 27-hydroxycholesterol. Plasma oxysterol concentrations were significantly lower in female than in male subjects. The Low HDL male group had higher 27-hydroxycholesterol than the High HDL male group. Cell cholesterol efflux rate was lower in Low HDL than in High HDL and related inversely with 27-hydroxycholesterol. Conclusion: As compared to High HDL, Low HDL men have increased 27-hydroxycholesterol plasma level that may circumvent their reduced cell cholesterol efflux rate.
conferenceObject EVIDENCE OF A PROTECTIVE LIPID PROFILE IN THE PRESENCE OF C.+83C > T SNP IN APOLIPOPROTEIN A-I GENE IN A HEALTHY BRAZILIAN POPULATION(2014) PARRA, E. Soler; ZAGO, V. H. Souza; SCHERRER, D. Zanetti; ALEXANDRE, F.; PANZOLDO, N. Baratella; NUNES, V. Sutti; NAKANDAKARE, E. R.; QUINTAO, E. C. R.; FARIA, E. Cotta deconferenceObject Interesterified Fats Induce Nonalcoholic Steatohepatitis or Enhance the Adipose Tissue and Adipocyte Hypertrophy(2014) LAVRADOR, Maria Silvia F.; AFONSO, Milessa S.; KOIKE, Marcia; BOMBO, Renata P.; CINTRA, Dennys E.; NUNES, Valeria S.; CATANOZI, Sergio; NAKANDAKARE, Edna R.; LOTTENBERG, Ana MariaconferenceObject Interesterified Fat Rich in Stearic Acid Increases Liver Neutrophil and M1 Macrophage Infiltration and Induces Nonalcoholic Steatohepatitis(2014) LAVRADOR, Maria Silvia F.; AFONSO, Milessa S.; KOIKE, Marcia; CINTRA, Dennys E.; BOMBO, Renata P.; NUNES, Valeria S.; CATANOZI, Sergio; NAKANDAKARE, Edna R.; LOTTENBERG, Ana Maria